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In Vitro and In Vivo Activities of Essential Oil from the Seed of Anethum graveolens L. against Candida spp.

Zeng H, Tian J, Zheng Y, Ban X, Zeng J, Mao Y, Wang Y - Evid Based Complement Alternat Med (2011)

Bottom Line: The essential oil produced from the seed of Anethum graveolens L. (Umbelliferae) was tested in vitro and in vivo anti-Candida activity.The anti-Candida activity was analyzed by microbiological and histological techniques and was compared with that of fluconazole (FCZ).The experimental results revealed that the tested essential oil is effective against vulvovaginal candidiasis in immunosuppressed mice.

View Article: PubMed Central - PubMed

Affiliation: The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT
The essential oil produced from the seed of Anethum graveolens L. (Umbelliferae) was tested in vitro and in vivo anti-Candida activity. The microbroth dilution method was used in the minimal inhibitory concentration (MIC), according to M27-A3 of the guidelines of the Clinical and Laboratory Standard Institute (CLSI). And then, efficacy evaluation of essential oil in the prophylaxis and treatment of experimental vaginal candidiasis was performed in immunosuppressed mice. The anti-Candida activity was analyzed by microbiological and histological techniques and was compared with that of fluconazole (FCZ). The results showed essential oil was active in vitro against all tested strains, with MICs ranging from 0.312 μL/mL (for C. tropicalis, C. parapsilosis, and C. krusei) to 0.625 μL/mL (for 6 isolated C. albicans strains). Essential oil (2% v/v) was highly efficacious in accelerating C. albicans 09-1555 clearance from experimentally infected mice vagina by prophylaxis and therapeutic treatments. In both therapeutic efficacy and prophylaxis studies, the histological findings confirmed the microbiological results. The experimental results revealed that the tested essential oil is effective against vulvovaginal candidiasis in immunosuppressed mice.

No MeSH data available.


Related in: MedlinePlus

Microscopic observation of therapeutic efficacy of essential oil versus FCZ against vaginal candidiasis in BALB/c mice on Post-infection day 4, day 8, day 15 (periodic acid-Schiff staining, 400x). CK: neither infected nor treated animals. Group 1: infected, untreated but not immunosuppressed animals. Group 2: immunosuppressed, inoculated intravaginally with C. albicans 09-1555, received excipient. Group T1: treated groups:immunosuppressed, infected animals received FCZ (20 μL at 100 μg/mL). Groups T2, T3, T4 treated groups: immunosuppressed, infected animals received essential oils (20 μL at 2% v/v, 1% v/v, 0.0625% v/v). The bar is 10 μm.
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fig4: Microscopic observation of therapeutic efficacy of essential oil versus FCZ against vaginal candidiasis in BALB/c mice on Post-infection day 4, day 8, day 15 (periodic acid-Schiff staining, 400x). CK: neither infected nor treated animals. Group 1: infected, untreated but not immunosuppressed animals. Group 2: immunosuppressed, inoculated intravaginally with C. albicans 09-1555, received excipient. Group T1: treated groups:immunosuppressed, infected animals received FCZ (20 μL at 100 μg/mL). Groups T2, T3, T4 treated groups: immunosuppressed, infected animals received essential oils (20 μL at 2% v/v, 1% v/v, 0.0625% v/v). The bar is 10 μm.

Mentions: Therapeutic treatment: vaginal sections of all the animals were also studied by light microscopy. Infected and untreated mice demonstrated the presence of C. albicans 09-1555 at the surface of the epithelium with desquamation of superficial layers, whereas in CK (neither infected nor treated animals), no Candida was found in the vaginal lumina (Figure 4).


In Vitro and In Vivo Activities of Essential Oil from the Seed of Anethum graveolens L. against Candida spp.

Zeng H, Tian J, Zheng Y, Ban X, Zeng J, Mao Y, Wang Y - Evid Based Complement Alternat Med (2011)

Microscopic observation of therapeutic efficacy of essential oil versus FCZ against vaginal candidiasis in BALB/c mice on Post-infection day 4, day 8, day 15 (periodic acid-Schiff staining, 400x). CK: neither infected nor treated animals. Group 1: infected, untreated but not immunosuppressed animals. Group 2: immunosuppressed, inoculated intravaginally with C. albicans 09-1555, received excipient. Group T1: treated groups:immunosuppressed, infected animals received FCZ (20 μL at 100 μg/mL). Groups T2, T3, T4 treated groups: immunosuppressed, infected animals received essential oils (20 μL at 2% v/v, 1% v/v, 0.0625% v/v). The bar is 10 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3119046&req=5

fig4: Microscopic observation of therapeutic efficacy of essential oil versus FCZ against vaginal candidiasis in BALB/c mice on Post-infection day 4, day 8, day 15 (periodic acid-Schiff staining, 400x). CK: neither infected nor treated animals. Group 1: infected, untreated but not immunosuppressed animals. Group 2: immunosuppressed, inoculated intravaginally with C. albicans 09-1555, received excipient. Group T1: treated groups:immunosuppressed, infected animals received FCZ (20 μL at 100 μg/mL). Groups T2, T3, T4 treated groups: immunosuppressed, infected animals received essential oils (20 μL at 2% v/v, 1% v/v, 0.0625% v/v). The bar is 10 μm.
Mentions: Therapeutic treatment: vaginal sections of all the animals were also studied by light microscopy. Infected and untreated mice demonstrated the presence of C. albicans 09-1555 at the surface of the epithelium with desquamation of superficial layers, whereas in CK (neither infected nor treated animals), no Candida was found in the vaginal lumina (Figure 4).

Bottom Line: The essential oil produced from the seed of Anethum graveolens L. (Umbelliferae) was tested in vitro and in vivo anti-Candida activity.The anti-Candida activity was analyzed by microbiological and histological techniques and was compared with that of fluconazole (FCZ).The experimental results revealed that the tested essential oil is effective against vulvovaginal candidiasis in immunosuppressed mice.

View Article: PubMed Central - PubMed

Affiliation: The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT
The essential oil produced from the seed of Anethum graveolens L. (Umbelliferae) was tested in vitro and in vivo anti-Candida activity. The microbroth dilution method was used in the minimal inhibitory concentration (MIC), according to M27-A3 of the guidelines of the Clinical and Laboratory Standard Institute (CLSI). And then, efficacy evaluation of essential oil in the prophylaxis and treatment of experimental vaginal candidiasis was performed in immunosuppressed mice. The anti-Candida activity was analyzed by microbiological and histological techniques and was compared with that of fluconazole (FCZ). The results showed essential oil was active in vitro against all tested strains, with MICs ranging from 0.312 μL/mL (for C. tropicalis, C. parapsilosis, and C. krusei) to 0.625 μL/mL (for 6 isolated C. albicans strains). Essential oil (2% v/v) was highly efficacious in accelerating C. albicans 09-1555 clearance from experimentally infected mice vagina by prophylaxis and therapeutic treatments. In both therapeutic efficacy and prophylaxis studies, the histological findings confirmed the microbiological results. The experimental results revealed that the tested essential oil is effective against vulvovaginal candidiasis in immunosuppressed mice.

No MeSH data available.


Related in: MedlinePlus