Limits...
In Vitro and In Vivo Activities of Essential Oil from the Seed of Anethum graveolens L. against Candida spp.

Zeng H, Tian J, Zheng Y, Ban X, Zeng J, Mao Y, Wang Y - Evid Based Complement Alternat Med (2011)

Bottom Line: The essential oil produced from the seed of Anethum graveolens L. (Umbelliferae) was tested in vitro and in vivo anti-Candida activity.The anti-Candida activity was analyzed by microbiological and histological techniques and was compared with that of fluconazole (FCZ).The experimental results revealed that the tested essential oil is effective against vulvovaginal candidiasis in immunosuppressed mice.

View Article: PubMed Central - PubMed

Affiliation: The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT
The essential oil produced from the seed of Anethum graveolens L. (Umbelliferae) was tested in vitro and in vivo anti-Candida activity. The microbroth dilution method was used in the minimal inhibitory concentration (MIC), according to M27-A3 of the guidelines of the Clinical and Laboratory Standard Institute (CLSI). And then, efficacy evaluation of essential oil in the prophylaxis and treatment of experimental vaginal candidiasis was performed in immunosuppressed mice. The anti-Candida activity was analyzed by microbiological and histological techniques and was compared with that of fluconazole (FCZ). The results showed essential oil was active in vitro against all tested strains, with MICs ranging from 0.312 μL/mL (for C. tropicalis, C. parapsilosis, and C. krusei) to 0.625 μL/mL (for 6 isolated C. albicans strains). Essential oil (2% v/v) was highly efficacious in accelerating C. albicans 09-1555 clearance from experimentally infected mice vagina by prophylaxis and therapeutic treatments. In both therapeutic efficacy and prophylaxis studies, the histological findings confirmed the microbiological results. The experimental results revealed that the tested essential oil is effective against vulvovaginal candidiasis in immunosuppressed mice.

No MeSH data available.


Related in: MedlinePlus

Microscopic observation of the prophylactic efficacy of essential oil versus FCZ against vaginal candidiasis in BALB/c mice on days 2, 8, and 15 after-infection (periodic acid-Schiff staining, 400x). CK: neither infected nor treated animals. Group 1: infected, untreated but not immunosuppressed animals. Group 2: immunosuppressed and inoculated intravaginally with C. albicans 09-1555, and animals that received excipient. Group P1: treated groups: immunosuppressed, infected animals that received FCZ (20 μL at 100 μg/mL). Groups P2, P3, P4: treated groups: immunosuppressed, infected animals that received essential oils (20 μL at 2, 1, and 0.0625% v/v).The bar is 10 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3119046&req=5

fig3: Microscopic observation of the prophylactic efficacy of essential oil versus FCZ against vaginal candidiasis in BALB/c mice on days 2, 8, and 15 after-infection (periodic acid-Schiff staining, 400x). CK: neither infected nor treated animals. Group 1: infected, untreated but not immunosuppressed animals. Group 2: immunosuppressed and inoculated intravaginally with C. albicans 09-1555, and animals that received excipient. Group P1: treated groups: immunosuppressed, infected animals that received FCZ (20 μL at 100 μg/mL). Groups P2, P3, P4: treated groups: immunosuppressed, infected animals that received essential oils (20 μL at 2, 1, and 0.0625% v/v).The bar is 10 μm.

Mentions: The infected mice from Groups P1–P4 (immunosuppressed and then infected and treated with FCZ and essential oil) all showed budding yeast and pseudohyphae in the vaginal luminal, on day 2 after inoculation (Figure 3). However, those from Group 2 (immunosuppressed infected and treated with excipient) showed large amounts of yeast and pseudohyphae in the vaginal luminal (Figure 3). Other groups showed yeast and pseudohyphae in the vaginal luminal dose dependently. Samples from Groups P1, P2, P3, and P4 received FCZ (100 μg/mL) and essential oils (2, 1, 0.0625% v/v) on day 8 after inoculation, less than that for Candida in the vaginal luminal (Figure 3), compared with Group 2 (Figure 3). On the 15th day after inoculation, Group P2 showed no Candida organisms detected by histological techniques (Figure 3) and it showed the same aspect as CK (neither infected nor treated animals), which was better than Group P1 (infected groups treated with FCZ).


In Vitro and In Vivo Activities of Essential Oil from the Seed of Anethum graveolens L. against Candida spp.

Zeng H, Tian J, Zheng Y, Ban X, Zeng J, Mao Y, Wang Y - Evid Based Complement Alternat Med (2011)

Microscopic observation of the prophylactic efficacy of essential oil versus FCZ against vaginal candidiasis in BALB/c mice on days 2, 8, and 15 after-infection (periodic acid-Schiff staining, 400x). CK: neither infected nor treated animals. Group 1: infected, untreated but not immunosuppressed animals. Group 2: immunosuppressed and inoculated intravaginally with C. albicans 09-1555, and animals that received excipient. Group P1: treated groups: immunosuppressed, infected animals that received FCZ (20 μL at 100 μg/mL). Groups P2, P3, P4: treated groups: immunosuppressed, infected animals that received essential oils (20 μL at 2, 1, and 0.0625% v/v).The bar is 10 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3119046&req=5

fig3: Microscopic observation of the prophylactic efficacy of essential oil versus FCZ against vaginal candidiasis in BALB/c mice on days 2, 8, and 15 after-infection (periodic acid-Schiff staining, 400x). CK: neither infected nor treated animals. Group 1: infected, untreated but not immunosuppressed animals. Group 2: immunosuppressed and inoculated intravaginally with C. albicans 09-1555, and animals that received excipient. Group P1: treated groups: immunosuppressed, infected animals that received FCZ (20 μL at 100 μg/mL). Groups P2, P3, P4: treated groups: immunosuppressed, infected animals that received essential oils (20 μL at 2, 1, and 0.0625% v/v).The bar is 10 μm.
Mentions: The infected mice from Groups P1–P4 (immunosuppressed and then infected and treated with FCZ and essential oil) all showed budding yeast and pseudohyphae in the vaginal luminal, on day 2 after inoculation (Figure 3). However, those from Group 2 (immunosuppressed infected and treated with excipient) showed large amounts of yeast and pseudohyphae in the vaginal luminal (Figure 3). Other groups showed yeast and pseudohyphae in the vaginal luminal dose dependently. Samples from Groups P1, P2, P3, and P4 received FCZ (100 μg/mL) and essential oils (2, 1, 0.0625% v/v) on day 8 after inoculation, less than that for Candida in the vaginal luminal (Figure 3), compared with Group 2 (Figure 3). On the 15th day after inoculation, Group P2 showed no Candida organisms detected by histological techniques (Figure 3) and it showed the same aspect as CK (neither infected nor treated animals), which was better than Group P1 (infected groups treated with FCZ).

Bottom Line: The essential oil produced from the seed of Anethum graveolens L. (Umbelliferae) was tested in vitro and in vivo anti-Candida activity.The anti-Candida activity was analyzed by microbiological and histological techniques and was compared with that of fluconazole (FCZ).The experimental results revealed that the tested essential oil is effective against vulvovaginal candidiasis in immunosuppressed mice.

View Article: PubMed Central - PubMed

Affiliation: The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT
The essential oil produced from the seed of Anethum graveolens L. (Umbelliferae) was tested in vitro and in vivo anti-Candida activity. The microbroth dilution method was used in the minimal inhibitory concentration (MIC), according to M27-A3 of the guidelines of the Clinical and Laboratory Standard Institute (CLSI). And then, efficacy evaluation of essential oil in the prophylaxis and treatment of experimental vaginal candidiasis was performed in immunosuppressed mice. The anti-Candida activity was analyzed by microbiological and histological techniques and was compared with that of fluconazole (FCZ). The results showed essential oil was active in vitro against all tested strains, with MICs ranging from 0.312 μL/mL (for C. tropicalis, C. parapsilosis, and C. krusei) to 0.625 μL/mL (for 6 isolated C. albicans strains). Essential oil (2% v/v) was highly efficacious in accelerating C. albicans 09-1555 clearance from experimentally infected mice vagina by prophylaxis and therapeutic treatments. In both therapeutic efficacy and prophylaxis studies, the histological findings confirmed the microbiological results. The experimental results revealed that the tested essential oil is effective against vulvovaginal candidiasis in immunosuppressed mice.

No MeSH data available.


Related in: MedlinePlus