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Development of Randomly Amplified Polymorphic DNA Based SCAR Marker for Identification of Ipomoea mauritiana Jacq (Convolvulaceae).

Devaiah K, Balasubramani SP, Venkatasubramanian P - Evid Based Complement Alternat Med (2011)

Bottom Line: Vidari is an Ayurvedic herbal drug used as aphrodisiac, galactagogue and is also used in the preparation of Chyavanaprash.DNA-based markers have been developed to distinguish I. mauritiana from the other Vidari candidates.The SCAR primers produced a specific 323-bp amplicon in authentic I. mauritiana and not in the allied species.

View Article: PubMed Central - PubMed

Affiliation: Centre for Pharmacognosy, Pharmaceutics and Pharmacology, Foundation for Revitalization of Local Health Traditions (FRLHT), via Yelhanka, Bangalore 560106, India.

ABSTRACT
Vidari is an Ayurvedic herbal drug used as aphrodisiac, galactagogue and is also used in the preparation of Chyavanaprash. Tubers of Ipomoea mauritiana Jacq. (Convolvulaceae), Pueraria tuberosa (Roxb. ex Willd.) DC (Fabaceae), Adenia hondala (Gaertn.) de Wilde (Passifloraceae) and pith of Cycas circinalis L. (Cycadaceae) are all traded in the name of Vidari, creating issues of botanical authenticity of the Ayurvedic raw drug. DNA-based markers have been developed to distinguish I. mauritiana from the other Vidari candidates. A putative 600-bp polymorphic sequence, specific to I. mauritiana was identified using randomly amplified polymorphic DNA (RAPD) technique. Furthermore, sequence characterized amplified region (SCAR) primers (IM1F and IM1R) were designed from the unique RAPD amplicon. The SCAR primers produced a specific 323-bp amplicon in authentic I. mauritiana and not in the allied species.

No MeSH data available.


Related in: MedlinePlus

Diagrammatic representation of RAPD-SCAR marker development to authenticate I. mauritiana Jacq.
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Related In: Results  -  Collection


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fig4: Diagrammatic representation of RAPD-SCAR marker development to authenticate I. mauritiana Jacq.

Mentions: In our RAPD analysis, significant genetic polymorphism was observed among I. mauritiana and its adulterants and substitutes. The SCAR primers designed using this sequence variation was found to be specific for I. mauritiana, making the technique more stringent and specific when compared with RAPD marker. The SCAR primers can be used to authenticate the identity of I. mauritiana from other traded “Vidari” candidates like C. circinalis, A. hondala and P. tuberosa. The complete process involved in developing RAPD-based SCAR marker for authentication of I. mauritiana is summarized in Figure 4. Such efficient, precise and sensitive techniques are required in quality control of raw drugs in the herbal industry.


Development of Randomly Amplified Polymorphic DNA Based SCAR Marker for Identification of Ipomoea mauritiana Jacq (Convolvulaceae).

Devaiah K, Balasubramani SP, Venkatasubramanian P - Evid Based Complement Alternat Med (2011)

Diagrammatic representation of RAPD-SCAR marker development to authenticate I. mauritiana Jacq.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3118933&req=5

fig4: Diagrammatic representation of RAPD-SCAR marker development to authenticate I. mauritiana Jacq.
Mentions: In our RAPD analysis, significant genetic polymorphism was observed among I. mauritiana and its adulterants and substitutes. The SCAR primers designed using this sequence variation was found to be specific for I. mauritiana, making the technique more stringent and specific when compared with RAPD marker. The SCAR primers can be used to authenticate the identity of I. mauritiana from other traded “Vidari” candidates like C. circinalis, A. hondala and P. tuberosa. The complete process involved in developing RAPD-based SCAR marker for authentication of I. mauritiana is summarized in Figure 4. Such efficient, precise and sensitive techniques are required in quality control of raw drugs in the herbal industry.

Bottom Line: Vidari is an Ayurvedic herbal drug used as aphrodisiac, galactagogue and is also used in the preparation of Chyavanaprash.DNA-based markers have been developed to distinguish I. mauritiana from the other Vidari candidates.The SCAR primers produced a specific 323-bp amplicon in authentic I. mauritiana and not in the allied species.

View Article: PubMed Central - PubMed

Affiliation: Centre for Pharmacognosy, Pharmaceutics and Pharmacology, Foundation for Revitalization of Local Health Traditions (FRLHT), via Yelhanka, Bangalore 560106, India.

ABSTRACT
Vidari is an Ayurvedic herbal drug used as aphrodisiac, galactagogue and is also used in the preparation of Chyavanaprash. Tubers of Ipomoea mauritiana Jacq. (Convolvulaceae), Pueraria tuberosa (Roxb. ex Willd.) DC (Fabaceae), Adenia hondala (Gaertn.) de Wilde (Passifloraceae) and pith of Cycas circinalis L. (Cycadaceae) are all traded in the name of Vidari, creating issues of botanical authenticity of the Ayurvedic raw drug. DNA-based markers have been developed to distinguish I. mauritiana from the other Vidari candidates. A putative 600-bp polymorphic sequence, specific to I. mauritiana was identified using randomly amplified polymorphic DNA (RAPD) technique. Furthermore, sequence characterized amplified region (SCAR) primers (IM1F and IM1R) were designed from the unique RAPD amplicon. The SCAR primers produced a specific 323-bp amplicon in authentic I. mauritiana and not in the allied species.

No MeSH data available.


Related in: MedlinePlus