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Development of Randomly Amplified Polymorphic DNA Based SCAR Marker for Identification of Ipomoea mauritiana Jacq (Convolvulaceae).

Devaiah K, Balasubramani SP, Venkatasubramanian P - Evid Based Complement Alternat Med (2011)

Bottom Line: Vidari is an Ayurvedic herbal drug used as aphrodisiac, galactagogue and is also used in the preparation of Chyavanaprash.DNA-based markers have been developed to distinguish I. mauritiana from the other Vidari candidates.The SCAR primers produced a specific 323-bp amplicon in authentic I. mauritiana and not in the allied species.

View Article: PubMed Central - PubMed

Affiliation: Centre for Pharmacognosy, Pharmaceutics and Pharmacology, Foundation for Revitalization of Local Health Traditions (FRLHT), via Yelhanka, Bangalore 560106, India.

ABSTRACT
Vidari is an Ayurvedic herbal drug used as aphrodisiac, galactagogue and is also used in the preparation of Chyavanaprash. Tubers of Ipomoea mauritiana Jacq. (Convolvulaceae), Pueraria tuberosa (Roxb. ex Willd.) DC (Fabaceae), Adenia hondala (Gaertn.) de Wilde (Passifloraceae) and pith of Cycas circinalis L. (Cycadaceae) are all traded in the name of Vidari, creating issues of botanical authenticity of the Ayurvedic raw drug. DNA-based markers have been developed to distinguish I. mauritiana from the other Vidari candidates. A putative 600-bp polymorphic sequence, specific to I. mauritiana was identified using randomly amplified polymorphic DNA (RAPD) technique. Furthermore, sequence characterized amplified region (SCAR) primers (IM1F and IM1R) were designed from the unique RAPD amplicon. The SCAR primers produced a specific 323-bp amplicon in authentic I. mauritiana and not in the allied species.

No MeSH data available.


Related in: MedlinePlus

Sequence of 600-bp polymorphic region of I. mauritiana, showing binding sites of RAPD (OPA 18) marker and SCAR Marker (IM1F and IM1R).
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fig2: Sequence of 600-bp polymorphic region of I. mauritiana, showing binding sites of RAPD (OPA 18) marker and SCAR Marker (IM1F and IM1R).

Mentions: Ipomoea mauritiana specific 600-bp polymorphic band was cloned into pDRIVE cloning vector and transformed into Qiagen EZ competent cells. The selected white colonies contained the required recombinant construct as was confirmed by reamplification with OPA18. The recombinant construct was sequenced using universal sequencing primers SP6 and T7. The sequencing reaction yielded a 601-bp stretch with the purified band from I. mauritiana (A_371; C_211; G_212; T_323) (Figure 2).


Development of Randomly Amplified Polymorphic DNA Based SCAR Marker for Identification of Ipomoea mauritiana Jacq (Convolvulaceae).

Devaiah K, Balasubramani SP, Venkatasubramanian P - Evid Based Complement Alternat Med (2011)

Sequence of 600-bp polymorphic region of I. mauritiana, showing binding sites of RAPD (OPA 18) marker and SCAR Marker (IM1F and IM1R).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3118933&req=5

fig2: Sequence of 600-bp polymorphic region of I. mauritiana, showing binding sites of RAPD (OPA 18) marker and SCAR Marker (IM1F and IM1R).
Mentions: Ipomoea mauritiana specific 600-bp polymorphic band was cloned into pDRIVE cloning vector and transformed into Qiagen EZ competent cells. The selected white colonies contained the required recombinant construct as was confirmed by reamplification with OPA18. The recombinant construct was sequenced using universal sequencing primers SP6 and T7. The sequencing reaction yielded a 601-bp stretch with the purified band from I. mauritiana (A_371; C_211; G_212; T_323) (Figure 2).

Bottom Line: Vidari is an Ayurvedic herbal drug used as aphrodisiac, galactagogue and is also used in the preparation of Chyavanaprash.DNA-based markers have been developed to distinguish I. mauritiana from the other Vidari candidates.The SCAR primers produced a specific 323-bp amplicon in authentic I. mauritiana and not in the allied species.

View Article: PubMed Central - PubMed

Affiliation: Centre for Pharmacognosy, Pharmaceutics and Pharmacology, Foundation for Revitalization of Local Health Traditions (FRLHT), via Yelhanka, Bangalore 560106, India.

ABSTRACT
Vidari is an Ayurvedic herbal drug used as aphrodisiac, galactagogue and is also used in the preparation of Chyavanaprash. Tubers of Ipomoea mauritiana Jacq. (Convolvulaceae), Pueraria tuberosa (Roxb. ex Willd.) DC (Fabaceae), Adenia hondala (Gaertn.) de Wilde (Passifloraceae) and pith of Cycas circinalis L. (Cycadaceae) are all traded in the name of Vidari, creating issues of botanical authenticity of the Ayurvedic raw drug. DNA-based markers have been developed to distinguish I. mauritiana from the other Vidari candidates. A putative 600-bp polymorphic sequence, specific to I. mauritiana was identified using randomly amplified polymorphic DNA (RAPD) technique. Furthermore, sequence characterized amplified region (SCAR) primers (IM1F and IM1R) were designed from the unique RAPD amplicon. The SCAR primers produced a specific 323-bp amplicon in authentic I. mauritiana and not in the allied species.

No MeSH data available.


Related in: MedlinePlus