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Efficacy of Brazilian Propolis against Herpes Simplex Virus Type 1 Infection in Mice and Their Modes of Antiherpetic Efficacies.

Shimizu T, Takeshita Y, Takamori Y, Kai H, Sawamura R, Yoshida H, Watanabe W, Tsutsumi A, Park YK, Yasukawa K, Matsuno K, Shiraki K, Kurokawa M - Evid Based Complement Alternat Med (2011)

Bottom Line: AF-07 and 08 significantly reduced virus titers in brain and/or skin on day 4 without toxicity, but AF-08 had no anti-HSV-1 activity in vitro.In addition, AF-06 and 07 possibly contain anti-HSV-1 components contributing to their efficacies.Such biological activities of Brazilian propolis may be useful to analyze its pharmacological actions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki 882-8508, Japan.

ABSTRACT
Ethanol extracts (AF-06, 07, and 08, 10 mg/kg) of Brazilian propolis were administered orally to cutaneously herpes simplex virus type 1 (HSV-1)-infected mice three times daily on days 0 to 6 after infection to evaluate their efficacies against HSV-1 infection and significantly limited development of herpetic skin lesions. AF-07 and 08 significantly reduced virus titers in brain and/or skin on day 4 without toxicity, but AF-08 had no anti-HSV-1 activity in vitro. AF-06 and 08 significantly enhanced delayed-type hypersensitivity (DTH) to inactivated HSV-1 antigen in infected mice. Oral AF-08-administration significantly augmented interferon (IFN)-γ production by HSV-1 antigen from splenocytes of HSV-1-infected mice, while direct exposure of splenocytes of infected mice to AF-06 significantly elevated IFN-γ production in vitro. Thus, AF-08 might have components that are active in vivo even after oral administration and those of AF-06 might be active only in vitro. Because DTH is a major host defense for intradermal HSV-1 infection, augmentation of DTH response by AF-06 or 08, directly or indirectly, respectively, may contribute to their efficacies against HSV-1 infection. In addition, AF-06 and 07 possibly contain anti-HSV-1 components contributing to their efficacies. Such biological activities of Brazilian propolis may be useful to analyze its pharmacological actions.

No MeSH data available.


Related in: MedlinePlus

Effects of AF-06, 07, and 08 on IFN-γ production from splenocytes by inactivated HSV antigen (Table 4).  Splenocytes were prepared from intradermally HSV-1-infected mice on day 4 and incubated in the presence of various concentrations of extracts (0, 0.1, 0.3, 1, 3, 10, and 30 μg/mL) and in the presence (closed columns) or absence (opened columns) of HSV antigen for 24 h.  IFN-γ levels in the culture supernatants were measured by ELISA as described in text.  *P < .05 versus HSV(−) by Student's t-test.  Bars indicate SEM.
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fig4: Effects of AF-06, 07, and 08 on IFN-γ production from splenocytes by inactivated HSV antigen (Table 4). Splenocytes were prepared from intradermally HSV-1-infected mice on day 4 and incubated in the presence of various concentrations of extracts (0, 0.1, 0.3, 1, 3, 10, and 30 μg/mL) and in the presence (closed columns) or absence (opened columns) of HSV antigen for 24 h. IFN-γ levels in the culture supernatants were measured by ELISA as described in text. *P < .05 versus HSV(−) by Student's t-test. Bars indicate SEM.

Mentions: On the other hand, IFN-γ production from splenocytes prepared from HSV-1-infected mice without administration of propolis extracts was examined in the presence of various concentrations of the extracts with or without inactivated HSV-1 antigen. As shown in Figure 4, AF-06 at 0.3 and 1 μg/mL significantly augmented IFN-γ production in the presence of inactivated HSV-1 antigen as compared with that in its absence. AF-06 exhibited the bell -shaped concentration dependency that is a characteristic of cytokine reaction by its direct stimulation to splenocytes. However, AF-07 and 08 failed to augment the production in the presence of the antigen at all concentrations examined.


Efficacy of Brazilian Propolis against Herpes Simplex Virus Type 1 Infection in Mice and Their Modes of Antiherpetic Efficacies.

Shimizu T, Takeshita Y, Takamori Y, Kai H, Sawamura R, Yoshida H, Watanabe W, Tsutsumi A, Park YK, Yasukawa K, Matsuno K, Shiraki K, Kurokawa M - Evid Based Complement Alternat Med (2011)

Effects of AF-06, 07, and 08 on IFN-γ production from splenocytes by inactivated HSV antigen (Table 4).  Splenocytes were prepared from intradermally HSV-1-infected mice on day 4 and incubated in the presence of various concentrations of extracts (0, 0.1, 0.3, 1, 3, 10, and 30 μg/mL) and in the presence (closed columns) or absence (opened columns) of HSV antigen for 24 h.  IFN-γ levels in the culture supernatants were measured by ELISA as described in text.  *P < .05 versus HSV(−) by Student's t-test.  Bars indicate SEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3118910&req=5

fig4: Effects of AF-06, 07, and 08 on IFN-γ production from splenocytes by inactivated HSV antigen (Table 4). Splenocytes were prepared from intradermally HSV-1-infected mice on day 4 and incubated in the presence of various concentrations of extracts (0, 0.1, 0.3, 1, 3, 10, and 30 μg/mL) and in the presence (closed columns) or absence (opened columns) of HSV antigen for 24 h. IFN-γ levels in the culture supernatants were measured by ELISA as described in text. *P < .05 versus HSV(−) by Student's t-test. Bars indicate SEM.
Mentions: On the other hand, IFN-γ production from splenocytes prepared from HSV-1-infected mice without administration of propolis extracts was examined in the presence of various concentrations of the extracts with or without inactivated HSV-1 antigen. As shown in Figure 4, AF-06 at 0.3 and 1 μg/mL significantly augmented IFN-γ production in the presence of inactivated HSV-1 antigen as compared with that in its absence. AF-06 exhibited the bell -shaped concentration dependency that is a characteristic of cytokine reaction by its direct stimulation to splenocytes. However, AF-07 and 08 failed to augment the production in the presence of the antigen at all concentrations examined.

Bottom Line: AF-07 and 08 significantly reduced virus titers in brain and/or skin on day 4 without toxicity, but AF-08 had no anti-HSV-1 activity in vitro.In addition, AF-06 and 07 possibly contain anti-HSV-1 components contributing to their efficacies.Such biological activities of Brazilian propolis may be useful to analyze its pharmacological actions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki 882-8508, Japan.

ABSTRACT
Ethanol extracts (AF-06, 07, and 08, 10 mg/kg) of Brazilian propolis were administered orally to cutaneously herpes simplex virus type 1 (HSV-1)-infected mice three times daily on days 0 to 6 after infection to evaluate their efficacies against HSV-1 infection and significantly limited development of herpetic skin lesions. AF-07 and 08 significantly reduced virus titers in brain and/or skin on day 4 without toxicity, but AF-08 had no anti-HSV-1 activity in vitro. AF-06 and 08 significantly enhanced delayed-type hypersensitivity (DTH) to inactivated HSV-1 antigen in infected mice. Oral AF-08-administration significantly augmented interferon (IFN)-γ production by HSV-1 antigen from splenocytes of HSV-1-infected mice, while direct exposure of splenocytes of infected mice to AF-06 significantly elevated IFN-γ production in vitro. Thus, AF-08 might have components that are active in vivo even after oral administration and those of AF-06 might be active only in vitro. Because DTH is a major host defense for intradermal HSV-1 infection, augmentation of DTH response by AF-06 or 08, directly or indirectly, respectively, may contribute to their efficacies against HSV-1 infection. In addition, AF-06 and 07 possibly contain anti-HSV-1 components contributing to their efficacies. Such biological activities of Brazilian propolis may be useful to analyze its pharmacological actions.

No MeSH data available.


Related in: MedlinePlus