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Efficacy of Brazilian Propolis against Herpes Simplex Virus Type 1 Infection in Mice and Their Modes of Antiherpetic Efficacies.

Shimizu T, Takeshita Y, Takamori Y, Kai H, Sawamura R, Yoshida H, Watanabe W, Tsutsumi A, Park YK, Yasukawa K, Matsuno K, Shiraki K, Kurokawa M - Evid Based Complement Alternat Med (2011)

Bottom Line: AF-07 and 08 significantly reduced virus titers in brain and/or skin on day 4 without toxicity, but AF-08 had no anti-HSV-1 activity in vitro.In addition, AF-06 and 07 possibly contain anti-HSV-1 components contributing to their efficacies.Such biological activities of Brazilian propolis may be useful to analyze its pharmacological actions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki 882-8508, Japan.

ABSTRACT
Ethanol extracts (AF-06, 07, and 08, 10 mg/kg) of Brazilian propolis were administered orally to cutaneously herpes simplex virus type 1 (HSV-1)-infected mice three times daily on days 0 to 6 after infection to evaluate their efficacies against HSV-1 infection and significantly limited development of herpetic skin lesions. AF-07 and 08 significantly reduced virus titers in brain and/or skin on day 4 without toxicity, but AF-08 had no anti-HSV-1 activity in vitro. AF-06 and 08 significantly enhanced delayed-type hypersensitivity (DTH) to inactivated HSV-1 antigen in infected mice. Oral AF-08-administration significantly augmented interferon (IFN)-γ production by HSV-1 antigen from splenocytes of HSV-1-infected mice, while direct exposure of splenocytes of infected mice to AF-06 significantly elevated IFN-γ production in vitro. Thus, AF-08 might have components that are active in vivo even after oral administration and those of AF-06 might be active only in vitro. Because DTH is a major host defense for intradermal HSV-1 infection, augmentation of DTH response by AF-06 or 08, directly or indirectly, respectively, may contribute to their efficacies against HSV-1 infection. In addition, AF-06 and 07 possibly contain anti-HSV-1 components contributing to their efficacies. Such biological activities of Brazilian propolis may be useful to analyze its pharmacological actions.

No MeSH data available.


Related in: MedlinePlus

Time course of skin reactions to HSV-1 antigen in mice infected intradermally with HSV-1.  Seven HSV-1-infected mice in a group were administered water (closed columns), AF-06 (opened columns), AF-07 (lightly dotted columns), or AF-08 (densely dotted columns), and the mean percent swelling was determined at 24, 36, and 48 h after injection of HSV antigen into footpads as described in text.  *P < .05 and **P < .01 versus control by Student's t-test.  Bars indicate SEM.
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fig3: Time course of skin reactions to HSV-1 antigen in mice infected intradermally with HSV-1. Seven HSV-1-infected mice in a group were administered water (closed columns), AF-06 (opened columns), AF-07 (lightly dotted columns), or AF-08 (densely dotted columns), and the mean percent swelling was determined at 24, 36, and 48 h after injection of HSV antigen into footpads as described in text. *P < .05 and **P < .01 versus control by Student's t-test. Bars indicate SEM.

Mentions: To evaluate the immunological effect of the three propolis extracts in mice infected with HSV-1, effects of the extracts on DTH reaction, which is one of the most important defense systems in intradermal HSV-1 infection, were examined [21–23]. As shown in Figure 3, AF-06 and 08 augmented the DTH skin reaction, a major immune defense response to intradermal HSV infection, at 36 h and/or 48 h after challenge of HSV-1 antigen but AF-07 did not.


Efficacy of Brazilian Propolis against Herpes Simplex Virus Type 1 Infection in Mice and Their Modes of Antiherpetic Efficacies.

Shimizu T, Takeshita Y, Takamori Y, Kai H, Sawamura R, Yoshida H, Watanabe W, Tsutsumi A, Park YK, Yasukawa K, Matsuno K, Shiraki K, Kurokawa M - Evid Based Complement Alternat Med (2011)

Time course of skin reactions to HSV-1 antigen in mice infected intradermally with HSV-1.  Seven HSV-1-infected mice in a group were administered water (closed columns), AF-06 (opened columns), AF-07 (lightly dotted columns), or AF-08 (densely dotted columns), and the mean percent swelling was determined at 24, 36, and 48 h after injection of HSV antigen into footpads as described in text.  *P < .05 and **P < .01 versus control by Student's t-test.  Bars indicate SEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3118910&req=5

fig3: Time course of skin reactions to HSV-1 antigen in mice infected intradermally with HSV-1. Seven HSV-1-infected mice in a group were administered water (closed columns), AF-06 (opened columns), AF-07 (lightly dotted columns), or AF-08 (densely dotted columns), and the mean percent swelling was determined at 24, 36, and 48 h after injection of HSV antigen into footpads as described in text. *P < .05 and **P < .01 versus control by Student's t-test. Bars indicate SEM.
Mentions: To evaluate the immunological effect of the three propolis extracts in mice infected with HSV-1, effects of the extracts on DTH reaction, which is one of the most important defense systems in intradermal HSV-1 infection, were examined [21–23]. As shown in Figure 3, AF-06 and 08 augmented the DTH skin reaction, a major immune defense response to intradermal HSV infection, at 36 h and/or 48 h after challenge of HSV-1 antigen but AF-07 did not.

Bottom Line: AF-07 and 08 significantly reduced virus titers in brain and/or skin on day 4 without toxicity, but AF-08 had no anti-HSV-1 activity in vitro.In addition, AF-06 and 07 possibly contain anti-HSV-1 components contributing to their efficacies.Such biological activities of Brazilian propolis may be useful to analyze its pharmacological actions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki 882-8508, Japan.

ABSTRACT
Ethanol extracts (AF-06, 07, and 08, 10 mg/kg) of Brazilian propolis were administered orally to cutaneously herpes simplex virus type 1 (HSV-1)-infected mice three times daily on days 0 to 6 after infection to evaluate their efficacies against HSV-1 infection and significantly limited development of herpetic skin lesions. AF-07 and 08 significantly reduced virus titers in brain and/or skin on day 4 without toxicity, but AF-08 had no anti-HSV-1 activity in vitro. AF-06 and 08 significantly enhanced delayed-type hypersensitivity (DTH) to inactivated HSV-1 antigen in infected mice. Oral AF-08-administration significantly augmented interferon (IFN)-γ production by HSV-1 antigen from splenocytes of HSV-1-infected mice, while direct exposure of splenocytes of infected mice to AF-06 significantly elevated IFN-γ production in vitro. Thus, AF-08 might have components that are active in vivo even after oral administration and those of AF-06 might be active only in vitro. Because DTH is a major host defense for intradermal HSV-1 infection, augmentation of DTH response by AF-06 or 08, directly or indirectly, respectively, may contribute to their efficacies against HSV-1 infection. In addition, AF-06 and 07 possibly contain anti-HSV-1 components contributing to their efficacies. Such biological activities of Brazilian propolis may be useful to analyze its pharmacological actions.

No MeSH data available.


Related in: MedlinePlus