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Prognostic significance of IL-6 and IL-8 ascites levels in ovarian cancer patients.

Lane D, Matte I, Rancourt C, Piché A - BMC Cancer (2011)

Bottom Line: The levels of IL-6 and IL-8 in ascites were significantly lower in patients that have received prior chemotherapy before the surgery (Mann-Whitney U test, P = 0.037 for IL-6 and P = 0.008 for IL-8).Univariate analysis revealed that high IL-6 ascites levels (P = 0.021), serum CA125 levels (P = 0.04) and stage IV (P = 0.009) were significantly correlated with shorter progression-free survival.Elevated IL-6, but not IL-8, ascites level is an independent predictor of shorter progression-free survival.

View Article: PubMed Central - HTML - PubMed

Affiliation: Département de Microbiologie et Infectiologie, Faculté de Médecine, Université de Sherbrooke, 3001, 12ième Avenue Nord, Sherbrooke, J1H 5N4, Canada.

ABSTRACT

Background: The acellular fraction of epithelial ovarian cancer (EOC) ascites promotes de novo resistance of tumor cells and thus supports the idea that tumor cells may survive in the surrounding protective microenvironment contributing to disease recurrence. Levels of the pro-inflammatory cytokines IL-6 and IL-8 are elevated in EOC ascites suggesting that they could play a role in tumor progression.

Methods: We measured IL-6 and IL-8 levels in the ascites of 39 patients with newly diagnosed EOC. Commercially available enzyme-linked immunosorbent assay (ELISA) was used to determine IL-6 and IL-8 ascites levels. Ascites cytokine levels were correlated with clinicopathological parameters and progression-free survival.

Results: Mean ascites levels for IL-6 and IL-8 were 6419 pg/ml (SEM: 1409 pg/ml) and 1408 pg/ml (SEM: 437 pg/ml) respectively. The levels of IL-6 and IL-8 in ascites were significantly lower in patients that have received prior chemotherapy before the surgery (Mann-Whitney U test, P = 0.037 for IL-6 and P = 0.008 for IL-8). Univariate analysis revealed that high IL-6 ascites levels (P = 0.021), serum CA125 levels (P = 0.04) and stage IV (P = 0.009) were significantly correlated with shorter progression-free survival. Including these variables in a multivariate analysis revealed that elevated IL-6 levels (P = 0.033) was an independent predictor of shorter progression-free survival.

Conclusion: Elevated IL-6, but not IL-8, ascites level is an independent predictor of shorter progression-free survival.

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Kaplan-Meier analysis of progression-free survival in patients with or without elevated levels of IL-6 and IL-8 in ascites.
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Figure 1: Kaplan-Meier analysis of progression-free survival in patients with or without elevated levels of IL-6 and IL-8 in ascites.

Mentions: Progression-free survival analysis in the overall patient population showed a shorter progression-free survival for patients with a median IL-6 ascites levels > 2662 pg/ml as compared with those presenting with a median IL-6 levels < 2662 pg/ml (log rank test, P = 0.021) (Figure 1). Median progression-free survival was 14 months for patients with high IL-6 levels versus 24 months for those with low IL-6. A cutoff value of 2662 pg/ml was selected according to the median ascites levels in the 39 patients. Patients with high levels of IL-6 were 2.3 times (95% CI, 1.09 - 4.84) more likely to have disease progression as compared to those with low (< 2662 pg/ml) IL-6 ascites levels. High levels of IL-8 (median ≥ 301 pg/ml) were not significantly associated with increased risk of disease progression or shorter progression-free survival (Figure 1).


Prognostic significance of IL-6 and IL-8 ascites levels in ovarian cancer patients.

Lane D, Matte I, Rancourt C, Piché A - BMC Cancer (2011)

Kaplan-Meier analysis of progression-free survival in patients with or without elevated levels of IL-6 and IL-8 in ascites.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3118896&req=5

Figure 1: Kaplan-Meier analysis of progression-free survival in patients with or without elevated levels of IL-6 and IL-8 in ascites.
Mentions: Progression-free survival analysis in the overall patient population showed a shorter progression-free survival for patients with a median IL-6 ascites levels > 2662 pg/ml as compared with those presenting with a median IL-6 levels < 2662 pg/ml (log rank test, P = 0.021) (Figure 1). Median progression-free survival was 14 months for patients with high IL-6 levels versus 24 months for those with low IL-6. A cutoff value of 2662 pg/ml was selected according to the median ascites levels in the 39 patients. Patients with high levels of IL-6 were 2.3 times (95% CI, 1.09 - 4.84) more likely to have disease progression as compared to those with low (< 2662 pg/ml) IL-6 ascites levels. High levels of IL-8 (median ≥ 301 pg/ml) were not significantly associated with increased risk of disease progression or shorter progression-free survival (Figure 1).

Bottom Line: The levels of IL-6 and IL-8 in ascites were significantly lower in patients that have received prior chemotherapy before the surgery (Mann-Whitney U test, P = 0.037 for IL-6 and P = 0.008 for IL-8).Univariate analysis revealed that high IL-6 ascites levels (P = 0.021), serum CA125 levels (P = 0.04) and stage IV (P = 0.009) were significantly correlated with shorter progression-free survival.Elevated IL-6, but not IL-8, ascites level is an independent predictor of shorter progression-free survival.

View Article: PubMed Central - HTML - PubMed

Affiliation: Département de Microbiologie et Infectiologie, Faculté de Médecine, Université de Sherbrooke, 3001, 12ième Avenue Nord, Sherbrooke, J1H 5N4, Canada.

ABSTRACT

Background: The acellular fraction of epithelial ovarian cancer (EOC) ascites promotes de novo resistance of tumor cells and thus supports the idea that tumor cells may survive in the surrounding protective microenvironment contributing to disease recurrence. Levels of the pro-inflammatory cytokines IL-6 and IL-8 are elevated in EOC ascites suggesting that they could play a role in tumor progression.

Methods: We measured IL-6 and IL-8 levels in the ascites of 39 patients with newly diagnosed EOC. Commercially available enzyme-linked immunosorbent assay (ELISA) was used to determine IL-6 and IL-8 ascites levels. Ascites cytokine levels were correlated with clinicopathological parameters and progression-free survival.

Results: Mean ascites levels for IL-6 and IL-8 were 6419 pg/ml (SEM: 1409 pg/ml) and 1408 pg/ml (SEM: 437 pg/ml) respectively. The levels of IL-6 and IL-8 in ascites were significantly lower in patients that have received prior chemotherapy before the surgery (Mann-Whitney U test, P = 0.037 for IL-6 and P = 0.008 for IL-8). Univariate analysis revealed that high IL-6 ascites levels (P = 0.021), serum CA125 levels (P = 0.04) and stage IV (P = 0.009) were significantly correlated with shorter progression-free survival. Including these variables in a multivariate analysis revealed that elevated IL-6 levels (P = 0.033) was an independent predictor of shorter progression-free survival.

Conclusion: Elevated IL-6, but not IL-8, ascites level is an independent predictor of shorter progression-free survival.

Show MeSH
Related in: MedlinePlus