Limits...
Epigenetic regulation of human β-defensin 2 and CC chemokine ligand 20 expression in gingival epithelial cells in response to oral bacteria.

Yin L, Chung WO - Mucosal Immunol (2011)

Bottom Line: Pretreatment with trichostatin A and sodium butyrate, which increase acetylation of chromatin histones, significantly enhanced the gene expression of antimicrobial proteins human β-defensin 2 (hBD2) and CC chemokine ligand 20 (CCL20) in response to both bacterial challenges.Furthermore, we observed a differential pattern of protein levels of H3K4me3, which has been associated with chromatin remodeling and activation of gene transcription, in response to P. gingivalis vs.F. nucleatum.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Biology, University of Washington, Seattle, Washington, USA. leiyin@u.washington.edu

ABSTRACT
Gingival epithelia utilize multiple signaling pathways to regulate innate immune responses to various oral bacteria, but little is understood about how these bacteria alter epithelial epigenetic status. In this study we report that DNA methyltransferase (DNMT1) and histone deacetylase expression were decreased in gingival epithelial cells treated with oral pathogen Porphyromonas gingivalis and nonpathogen Fusobacterium nucleatum. Pretreatment with trichostatin A and sodium butyrate, which increase acetylation of chromatin histones, significantly enhanced the gene expression of antimicrobial proteins human β-defensin 2 (hBD2) and CC chemokine ligand 20 (CCL20) in response to both bacterial challenges. Pretreatment with DNMT inhibitor 5'-azacytidine increased hBD2 and CCL20 expression in response to F. nucleatum, but not to P. gingivalis. Furthermore, we observed a differential pattern of protein levels of H3K4me3, which has been associated with chromatin remodeling and activation of gene transcription, in response to P. gingivalis vs. F. nucleatum. This study provides a new insight into the bacteria-specific innate immune responses via epigenetic regulation.

Show MeSH

Related in: MedlinePlus

Relevant gene networks based on literature co-occurrence. We submitted queried genes DEFB4 (human β-defensin 2 (hBD2)), CC chemokine ligand 20 (CCL20), interleukin-8 (IL-8), and methylated genes in Table 1 to the PubGene and identified coexpressed possibility based on the literature co-occurrence. Red color represents query genes, purple color represents the first-order literature, and black color represents the second-order literature. The color reproduction of this figure is available on the html full text version of the manuscript.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3118861&req=5

fig8: Relevant gene networks based on literature co-occurrence. We submitted queried genes DEFB4 (human β-defensin 2 (hBD2)), CC chemokine ligand 20 (CCL20), interleukin-8 (IL-8), and methylated genes in Table 1 to the PubGene and identified coexpressed possibility based on the literature co-occurrence. Red color represents query genes, purple color represents the first-order literature, and black color represents the second-order literature. The color reproduction of this figure is available on the html full text version of the manuscript.

Mentions: We have observed that hBD2, CCL20, and chemokine IL-8 are differentially modulated via epigenetic mechanism, and also found that multiple genes are differentially methylated after exposure to P. gingivalis or F. nucleatum. In order to further understand biological correlation among the genes that respond to the bacterial treatments, we used PubGene to identify biological relationship based on literature co-occurrence index (Figure 8). The queried genes DEFB4 (hBD2), CCL20, and IL-8 are strongly associated with the genes that showed hypermethylation (ELA2, TRL2, STAT5A, IL-12A, and GATA3) based on literature co-citation. This illustrates the complex coregulatory patterns of tested genes, and highlights the biological relevance for the tested genes.


Epigenetic regulation of human β-defensin 2 and CC chemokine ligand 20 expression in gingival epithelial cells in response to oral bacteria.

Yin L, Chung WO - Mucosal Immunol (2011)

Relevant gene networks based on literature co-occurrence. We submitted queried genes DEFB4 (human β-defensin 2 (hBD2)), CC chemokine ligand 20 (CCL20), interleukin-8 (IL-8), and methylated genes in Table 1 to the PubGene and identified coexpressed possibility based on the literature co-occurrence. Red color represents query genes, purple color represents the first-order literature, and black color represents the second-order literature. The color reproduction of this figure is available on the html full text version of the manuscript.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3118861&req=5

fig8: Relevant gene networks based on literature co-occurrence. We submitted queried genes DEFB4 (human β-defensin 2 (hBD2)), CC chemokine ligand 20 (CCL20), interleukin-8 (IL-8), and methylated genes in Table 1 to the PubGene and identified coexpressed possibility based on the literature co-occurrence. Red color represents query genes, purple color represents the first-order literature, and black color represents the second-order literature. The color reproduction of this figure is available on the html full text version of the manuscript.
Mentions: We have observed that hBD2, CCL20, and chemokine IL-8 are differentially modulated via epigenetic mechanism, and also found that multiple genes are differentially methylated after exposure to P. gingivalis or F. nucleatum. In order to further understand biological correlation among the genes that respond to the bacterial treatments, we used PubGene to identify biological relationship based on literature co-occurrence index (Figure 8). The queried genes DEFB4 (hBD2), CCL20, and IL-8 are strongly associated with the genes that showed hypermethylation (ELA2, TRL2, STAT5A, IL-12A, and GATA3) based on literature co-citation. This illustrates the complex coregulatory patterns of tested genes, and highlights the biological relevance for the tested genes.

Bottom Line: Pretreatment with trichostatin A and sodium butyrate, which increase acetylation of chromatin histones, significantly enhanced the gene expression of antimicrobial proteins human β-defensin 2 (hBD2) and CC chemokine ligand 20 (CCL20) in response to both bacterial challenges.Furthermore, we observed a differential pattern of protein levels of H3K4me3, which has been associated with chromatin remodeling and activation of gene transcription, in response to P. gingivalis vs.F. nucleatum.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Biology, University of Washington, Seattle, Washington, USA. leiyin@u.washington.edu

ABSTRACT
Gingival epithelia utilize multiple signaling pathways to regulate innate immune responses to various oral bacteria, but little is understood about how these bacteria alter epithelial epigenetic status. In this study we report that DNA methyltransferase (DNMT1) and histone deacetylase expression were decreased in gingival epithelial cells treated with oral pathogen Porphyromonas gingivalis and nonpathogen Fusobacterium nucleatum. Pretreatment with trichostatin A and sodium butyrate, which increase acetylation of chromatin histones, significantly enhanced the gene expression of antimicrobial proteins human β-defensin 2 (hBD2) and CC chemokine ligand 20 (CCL20) in response to both bacterial challenges. Pretreatment with DNMT inhibitor 5'-azacytidine increased hBD2 and CCL20 expression in response to F. nucleatum, but not to P. gingivalis. Furthermore, we observed a differential pattern of protein levels of H3K4me3, which has been associated with chromatin remodeling and activation of gene transcription, in response to P. gingivalis vs. F. nucleatum. This study provides a new insight into the bacteria-specific innate immune responses via epigenetic regulation.

Show MeSH
Related in: MedlinePlus