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Viability of bacteria in dental calculus - A microbiological study.

Moolya NN, Thakur S, Ravindra S, Setty SB, Kulkarni R, Hallikeri K - J Indian Soc Periodontol (2010)

Bottom Line: Supra and subgingival calculus were harvested from 30 subjects having clinical evidence of chronic inflammatory periodontal disease and were divided into two groups.Samples from both groups were immediately transported to the Department of Microbiology for gram staining, acridine orange staining, bacterial culture and to the Department of Oral Pathology for dark field microscopy.Bacterial culture revealed presence of a variety of aerobic organisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, Dr. D. Y. Patil Dental College, Navi Mumbai, Maharashtra, India.

ABSTRACT

Aim: The aim of this study was (1) To investigate the viability of bacteria within supragingival and subgingival calculus, (2) To examine motility of bacteria, and (3) To identify bacterial morphotypes in calculus.

Materials and methods: Supra and subgingival calculus were harvested from 30 subjects having clinical evidence of chronic inflammatory periodontal disease and were divided into two groups. Samples from both groups were immediately transported to the Department of Microbiology for gram staining, acridine orange staining, bacterial culture and to the Department of Oral Pathology for dark field microscopy.

Results: Gram staining revealed presence of bacteria within the samples.Dark field microscopic examination revealed presence of filamentous organisms, spirochetes, and motile short bacilli. Acridine orange fluorescent stain showed that the viable bacteria appeared apple green. Bacterial culture revealed presence of a variety of aerobic organisms.

Conclusion: From the results, it appeared that viable bacteria were present within calculus especially within internal channels and lacunae.

No MeSH data available.


Related in: MedlinePlus

Acridine orange staining showing apple green colored appearance of bacteria
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Figure 5: Acridine orange staining showing apple green colored appearance of bacteria

Mentions: After application of the acridine orange fluorescent stain both viable bacteria appeared apple green. Viable bacteria which appeared as small dots or areas of bright green fluorescence were detected in calculus samples. All 12 samples demonstrated presence of viable bacteria [Figure 5].


Viability of bacteria in dental calculus - A microbiological study.

Moolya NN, Thakur S, Ravindra S, Setty SB, Kulkarni R, Hallikeri K - J Indian Soc Periodontol (2010)

Acridine orange staining showing apple green colored appearance of bacteria
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3118071&req=5

Figure 5: Acridine orange staining showing apple green colored appearance of bacteria
Mentions: After application of the acridine orange fluorescent stain both viable bacteria appeared apple green. Viable bacteria which appeared as small dots or areas of bright green fluorescence were detected in calculus samples. All 12 samples demonstrated presence of viable bacteria [Figure 5].

Bottom Line: Supra and subgingival calculus were harvested from 30 subjects having clinical evidence of chronic inflammatory periodontal disease and were divided into two groups.Samples from both groups were immediately transported to the Department of Microbiology for gram staining, acridine orange staining, bacterial culture and to the Department of Oral Pathology for dark field microscopy.Bacterial culture revealed presence of a variety of aerobic organisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, Dr. D. Y. Patil Dental College, Navi Mumbai, Maharashtra, India.

ABSTRACT

Aim: The aim of this study was (1) To investigate the viability of bacteria within supragingival and subgingival calculus, (2) To examine motility of bacteria, and (3) To identify bacterial morphotypes in calculus.

Materials and methods: Supra and subgingival calculus were harvested from 30 subjects having clinical evidence of chronic inflammatory periodontal disease and were divided into two groups. Samples from both groups were immediately transported to the Department of Microbiology for gram staining, acridine orange staining, bacterial culture and to the Department of Oral Pathology for dark field microscopy.

Results: Gram staining revealed presence of bacteria within the samples.Dark field microscopic examination revealed presence of filamentous organisms, spirochetes, and motile short bacilli. Acridine orange fluorescent stain showed that the viable bacteria appeared apple green. Bacterial culture revealed presence of a variety of aerobic organisms.

Conclusion: From the results, it appeared that viable bacteria were present within calculus especially within internal channels and lacunae.

No MeSH data available.


Related in: MedlinePlus