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Viability of bacteria in dental calculus - A microbiological study.

Moolya NN, Thakur S, Ravindra S, Setty SB, Kulkarni R, Hallikeri K - J Indian Soc Periodontol (2010)

Bottom Line: Supra and subgingival calculus were harvested from 30 subjects having clinical evidence of chronic inflammatory periodontal disease and were divided into two groups.Samples from both groups were immediately transported to the Department of Microbiology for gram staining, acridine orange staining, bacterial culture and to the Department of Oral Pathology for dark field microscopy.Bacterial culture revealed presence of a variety of aerobic organisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, Dr. D. Y. Patil Dental College, Navi Mumbai, Maharashtra, India.

ABSTRACT

Aim: The aim of this study was (1) To investigate the viability of bacteria within supragingival and subgingival calculus, (2) To examine motility of bacteria, and (3) To identify bacterial morphotypes in calculus.

Materials and methods: Supra and subgingival calculus were harvested from 30 subjects having clinical evidence of chronic inflammatory periodontal disease and were divided into two groups. Samples from both groups were immediately transported to the Department of Microbiology for gram staining, acridine orange staining, bacterial culture and to the Department of Oral Pathology for dark field microscopy.

Results: Gram staining revealed presence of bacteria within the samples.Dark field microscopic examination revealed presence of filamentous organisms, spirochetes, and motile short bacilli. Acridine orange fluorescent stain showed that the viable bacteria appeared apple green. Bacterial culture revealed presence of a variety of aerobic organisms.

Conclusion: From the results, it appeared that viable bacteria were present within calculus especially within internal channels and lacunae.

No MeSH data available.


Related in: MedlinePlus

Dark field microscopic imaging showing presence of rods and cocci
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Figure 4: Dark field microscopic imaging showing presence of rods and cocci

Mentions: Dark field microscopic examination revealed the presence of filamentous organisms, spirochetes, and motile short bacilli. Motility of organisms was appreciated in all the 12 samples. No difference was seen between the groups [Figure 4].


Viability of bacteria in dental calculus - A microbiological study.

Moolya NN, Thakur S, Ravindra S, Setty SB, Kulkarni R, Hallikeri K - J Indian Soc Periodontol (2010)

Dark field microscopic imaging showing presence of rods and cocci
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3118071&req=5

Figure 4: Dark field microscopic imaging showing presence of rods and cocci
Mentions: Dark field microscopic examination revealed the presence of filamentous organisms, spirochetes, and motile short bacilli. Motility of organisms was appreciated in all the 12 samples. No difference was seen between the groups [Figure 4].

Bottom Line: Supra and subgingival calculus were harvested from 30 subjects having clinical evidence of chronic inflammatory periodontal disease and were divided into two groups.Samples from both groups were immediately transported to the Department of Microbiology for gram staining, acridine orange staining, bacterial culture and to the Department of Oral Pathology for dark field microscopy.Bacterial culture revealed presence of a variety of aerobic organisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, Dr. D. Y. Patil Dental College, Navi Mumbai, Maharashtra, India.

ABSTRACT

Aim: The aim of this study was (1) To investigate the viability of bacteria within supragingival and subgingival calculus, (2) To examine motility of bacteria, and (3) To identify bacterial morphotypes in calculus.

Materials and methods: Supra and subgingival calculus were harvested from 30 subjects having clinical evidence of chronic inflammatory periodontal disease and were divided into two groups. Samples from both groups were immediately transported to the Department of Microbiology for gram staining, acridine orange staining, bacterial culture and to the Department of Oral Pathology for dark field microscopy.

Results: Gram staining revealed presence of bacteria within the samples.Dark field microscopic examination revealed presence of filamentous organisms, spirochetes, and motile short bacilli. Acridine orange fluorescent stain showed that the viable bacteria appeared apple green. Bacterial culture revealed presence of a variety of aerobic organisms.

Conclusion: From the results, it appeared that viable bacteria were present within calculus especially within internal channels and lacunae.

No MeSH data available.


Related in: MedlinePlus