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Efficacy of a chairside diagnostic test kit for estimation of C-reactive protein levels in periodontal disease.

Nagarale G, Ravindra S, Thakur S, Setty S - J Indian Soc Periodontol (2010)

Bottom Line: Serum levels of CRP were determined by using a latex slide agglutination method with commercially available kit with lower detection limit of 6 mg/L.CRP was negative in all the 15 subjects in groups A and B at baseline, 7th and 30th day.CRP was positive only in 2 subjects in Group C at baseline and 7th day.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, S.D.M. College of Dental Sciences and Hospital, Dharwad, Karnataka, India.

ABSTRACT

Background: C-reactive protein [CRP] levels increase to hundreds of mg/mL within hours following infection. Studies have shown that serum CRP levels were elevated in periodontal disease. However, in all the previous studies, CRP levels were measured by using high-sensitivity CRP assay kits with minimal detection limits of 0.1 to 3 mg/L, which was much below the normal value of 10 mg/L. These high-sensitivity CRP assays need a proper laboratory setup, and these methods cannot be used as a routine chair-side test in the dental office.

Aim: The purpose of this study was to investigate the serum CRP levels in subjects with periodontal disease by using a rapid chair-side diagnostic test kit with a lower detection limit of 6 mg/L and to compare the CRP levels before and after periodontal therapy.

Materials and methods: A total of 45 systemically healthy subjects were selected for the study. Subjects were divided into three groups: group A: healthy controls, group B: gingivitis, group C: periodontitis. Serum levels of CRP were determined by using a latex slide agglutination method with commercially available kit with lower detection limit of 6 mg/L.

Results: CRP was negative in all the 15 subjects in groups A and B at baseline, 7th and 30th day. CRP was positive only in 2 subjects in Group C at baseline and 7th day.

Conclusion: Estimation of serum CRP by using a rapid chair-side diagnostic test kit is not of any significance in subjects with periodontitis.

No MeSH data available.


Related in: MedlinePlus

Commercially available test kit for estimation of CRP
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Figure 2: Commercially available test kit for estimation of CRP

Mentions: Venous blood samples were obtained from the antecubital vein by venipuncture using a standard 2-mL syringe from each subject in all the 3 groups at baseline; and on the 7th day and 30th day post-baseline, blood samples were obtained only from subjects in groups B and C. Samples were allowed to coagulate for 1 to 2 hours at room temperature in a serum-collecting tube [Figure 1]. Serum was then separated by using micropipette. Serum levels of CRP were determined by using a latex slide agglutination method with commercially available kit obtained from Span Diagnostic Ltd. [Figures 2 and 3], as it has the advantage of rapid performance (within 2 minutes) in comparison to other methods for detection of CRP.[6–9] Since the lower detection limit of CRP was 6 μg/mL, subjects with CRP values <6 μg/mL were regarded sero-negative. After recording baseline clinical and biochemical measurements, only oral hygiene instructions were given to subjects belonging to group A. Full-mouth scaling and root planing was carried out in subjects belonging to groups B and C. Recall visits were scheduled for all the subjects belonging to groups B and C on 7th and 30th day to record both clinical and biochemical measurements. Descriptive statistics included mean and standard deviation for each of the measured clinical parameters. Clinical parameters at baseline and reassessment were compared using t tests. Since CRP levels were negative in most of the cases and as there were no variables present, CRP values were not subjected to statistical analysis.


Efficacy of a chairside diagnostic test kit for estimation of C-reactive protein levels in periodontal disease.

Nagarale G, Ravindra S, Thakur S, Setty S - J Indian Soc Periodontol (2010)

Commercially available test kit for estimation of CRP
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3118069&req=5

Figure 2: Commercially available test kit for estimation of CRP
Mentions: Venous blood samples were obtained from the antecubital vein by venipuncture using a standard 2-mL syringe from each subject in all the 3 groups at baseline; and on the 7th day and 30th day post-baseline, blood samples were obtained only from subjects in groups B and C. Samples were allowed to coagulate for 1 to 2 hours at room temperature in a serum-collecting tube [Figure 1]. Serum was then separated by using micropipette. Serum levels of CRP were determined by using a latex slide agglutination method with commercially available kit obtained from Span Diagnostic Ltd. [Figures 2 and 3], as it has the advantage of rapid performance (within 2 minutes) in comparison to other methods for detection of CRP.[6–9] Since the lower detection limit of CRP was 6 μg/mL, subjects with CRP values <6 μg/mL were regarded sero-negative. After recording baseline clinical and biochemical measurements, only oral hygiene instructions were given to subjects belonging to group A. Full-mouth scaling and root planing was carried out in subjects belonging to groups B and C. Recall visits were scheduled for all the subjects belonging to groups B and C on 7th and 30th day to record both clinical and biochemical measurements. Descriptive statistics included mean and standard deviation for each of the measured clinical parameters. Clinical parameters at baseline and reassessment were compared using t tests. Since CRP levels were negative in most of the cases and as there were no variables present, CRP values were not subjected to statistical analysis.

Bottom Line: Serum levels of CRP were determined by using a latex slide agglutination method with commercially available kit with lower detection limit of 6 mg/L.CRP was negative in all the 15 subjects in groups A and B at baseline, 7th and 30th day.CRP was positive only in 2 subjects in Group C at baseline and 7th day.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, S.D.M. College of Dental Sciences and Hospital, Dharwad, Karnataka, India.

ABSTRACT

Background: C-reactive protein [CRP] levels increase to hundreds of mg/mL within hours following infection. Studies have shown that serum CRP levels were elevated in periodontal disease. However, in all the previous studies, CRP levels were measured by using high-sensitivity CRP assay kits with minimal detection limits of 0.1 to 3 mg/L, which was much below the normal value of 10 mg/L. These high-sensitivity CRP assays need a proper laboratory setup, and these methods cannot be used as a routine chair-side test in the dental office.

Aim: The purpose of this study was to investigate the serum CRP levels in subjects with periodontal disease by using a rapid chair-side diagnostic test kit with a lower detection limit of 6 mg/L and to compare the CRP levels before and after periodontal therapy.

Materials and methods: A total of 45 systemically healthy subjects were selected for the study. Subjects were divided into three groups: group A: healthy controls, group B: gingivitis, group C: periodontitis. Serum levels of CRP were determined by using a latex slide agglutination method with commercially available kit with lower detection limit of 6 mg/L.

Results: CRP was negative in all the 15 subjects in groups A and B at baseline, 7th and 30th day. CRP was positive only in 2 subjects in Group C at baseline and 7th day.

Conclusion: Estimation of serum CRP by using a rapid chair-side diagnostic test kit is not of any significance in subjects with periodontitis.

No MeSH data available.


Related in: MedlinePlus