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Intramyocellular lipid droplets increase with progression of cachexia in cancer patients.

Stephens NA, Skipworth RJ, Macdonald AJ, Greig CA, Ross JA, Fearon KC - J Cachexia Sarcopenia Muscle (2011)

Bottom Line: We hypothesised that due to the phenotype associated with cancer cachexia, there would exist an association between increasing weight loss and the number/size of intramyocellular lipid droplets.The number of intramyocellular lipid droplets and lipid droplet diameter were calculated from the TEM images.Mean lipid droplet count correlated positively with the severity of weight loss (R = 0.51, p = 0.025) and negatively with CT-derived measures of intermuscular fat (R = -0.53, p = 0.022) and visceral fat (R = -0.51, p = 0.029).

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Surgical Sciences (Surgery), School of Clinical Sciences and Community Health, University of Edinburgh, Royal Infirmary, 51 Little France Crescent, Edinburgh, EH16 4SA UK.

ABSTRACT
BACKGROUND: Intramyocellular lipids are an important source of fuel for mitochondrial fat oxidation and play an important role in intramuscular lipid homeostasis. We hypothesised that due to the phenotype associated with cancer cachexia, there would exist an association between increasing weight loss and the number/size of intramyocellular lipid droplets. METHODS: Nineteen cancer patients and 6 controls undergoing surgery were recruited. A rectus abdominis biopsy was performed and processed for transmission electron microscopy (TEM). The number of intramyocellular lipid droplets and lipid droplet diameter were calculated from the TEM images. CT scans, performed as part of patients' routine care, were analysed to determine amount of adipose (intermuscular, visceral and subcutaneous) and muscle tissue. RESULTS: Compared with controls, cancer patients had increased numbers of lipid droplets (mean (SD) 1.8 (1.9) vs. 6.4 (9.1) per ×2,650 field, respectively, p = 0.036). Mean (SD) lipid droplet diameter was also higher in cancer patients compared with controls (0.42 (0.13) vs. 0.24 (0.21) μm, p = 0.015). Mean lipid droplet count correlated positively with the severity of weight loss (R = 0.51, p = 0.025) and negatively with CT-derived measures of intermuscular fat (R = -0.53, p = 0.022) and visceral fat (R = -0.51, p = 0.029). CONCLUSIONS: This study suggests that the number and size of intramyocellular lipid droplets is increased in the presence of cancer and increases further with weight loss/loss of adipose mass in other body compartments.

No MeSH data available.


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Percentage weight loss versus lipid droplet number. There was a significant positive correlation between percentage weight loss and LD number (R = 0.51, p = 0.025; Pearson's correlation, two tailed). There was no significant difference in LD number according to tumour site (one-way ANOVA, p = 0.559). LD lipid droplet
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Fig2: Percentage weight loss versus lipid droplet number. There was a significant positive correlation between percentage weight loss and LD number (R = 0.51, p = 0.025; Pearson's correlation, two tailed). There was no significant difference in LD number according to tumour site (one-way ANOVA, p = 0.559). LD lipid droplet

Mentions: The average lipid droplet number correlated positively with percentage weight loss (R = 0.51, p = 0.025, Fig. 2), but there was no relationship between weight loss and lipid droplet diameter (R = 0.15, p = 0.535). MAMC, arm muscle CSA and CT-derived muscle mass did not correlate significantly with either lipid droplet count or lipid droplet diameter. Both weight (R = −0.61, p = 0.007) and BMI (R = −0.46, p = 0.050) correlated negatively with the number of lipid droplets. There were significantly greater numbers of lipid droplets in patients with lower CT-derived measures of intermuscular fat (R = −0.53, p = 0.022) and visceral fat (R = −0.51, p = 0.029), and there was also a trend towards an association between droplet number and subcutaneous fat (R = −0.46, p = 0.055). No relationship was demonstrated between patients' age and number of lipid droplets (R = −0.30, p = 0.209) or between the number of lipid droplets and the lipid droplet diameter (R = 0.34, p = 0.152). There were no significant associations between the number of lipid droplets and plasma levels of albumin (R = −0.28, p = 0.256), CRP (R = −0.10, p = 0.695), insulin (R = −0.26, p = 0.391), glucose (R = −0.03, p = 0.926), cortisol (R = −0.13, p = 0.633), HOMA-IR (R = −0.38, p = 0.280) or cortisol/insulin ratio (R = 0.09, p = 0.783).Fig. 2


Intramyocellular lipid droplets increase with progression of cachexia in cancer patients.

Stephens NA, Skipworth RJ, Macdonald AJ, Greig CA, Ross JA, Fearon KC - J Cachexia Sarcopenia Muscle (2011)

Percentage weight loss versus lipid droplet number. There was a significant positive correlation between percentage weight loss and LD number (R = 0.51, p = 0.025; Pearson's correlation, two tailed). There was no significant difference in LD number according to tumour site (one-way ANOVA, p = 0.559). LD lipid droplet
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Related In: Results  -  Collection

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Fig2: Percentage weight loss versus lipid droplet number. There was a significant positive correlation between percentage weight loss and LD number (R = 0.51, p = 0.025; Pearson's correlation, two tailed). There was no significant difference in LD number according to tumour site (one-way ANOVA, p = 0.559). LD lipid droplet
Mentions: The average lipid droplet number correlated positively with percentage weight loss (R = 0.51, p = 0.025, Fig. 2), but there was no relationship between weight loss and lipid droplet diameter (R = 0.15, p = 0.535). MAMC, arm muscle CSA and CT-derived muscle mass did not correlate significantly with either lipid droplet count or lipid droplet diameter. Both weight (R = −0.61, p = 0.007) and BMI (R = −0.46, p = 0.050) correlated negatively with the number of lipid droplets. There were significantly greater numbers of lipid droplets in patients with lower CT-derived measures of intermuscular fat (R = −0.53, p = 0.022) and visceral fat (R = −0.51, p = 0.029), and there was also a trend towards an association between droplet number and subcutaneous fat (R = −0.46, p = 0.055). No relationship was demonstrated between patients' age and number of lipid droplets (R = −0.30, p = 0.209) or between the number of lipid droplets and the lipid droplet diameter (R = 0.34, p = 0.152). There were no significant associations between the number of lipid droplets and plasma levels of albumin (R = −0.28, p = 0.256), CRP (R = −0.10, p = 0.695), insulin (R = −0.26, p = 0.391), glucose (R = −0.03, p = 0.926), cortisol (R = −0.13, p = 0.633), HOMA-IR (R = −0.38, p = 0.280) or cortisol/insulin ratio (R = 0.09, p = 0.783).Fig. 2

Bottom Line: We hypothesised that due to the phenotype associated with cancer cachexia, there would exist an association between increasing weight loss and the number/size of intramyocellular lipid droplets.The number of intramyocellular lipid droplets and lipid droplet diameter were calculated from the TEM images.Mean lipid droplet count correlated positively with the severity of weight loss (R = 0.51, p = 0.025) and negatively with CT-derived measures of intermuscular fat (R = -0.53, p = 0.022) and visceral fat (R = -0.51, p = 0.029).

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Surgical Sciences (Surgery), School of Clinical Sciences and Community Health, University of Edinburgh, Royal Infirmary, 51 Little France Crescent, Edinburgh, EH16 4SA UK.

ABSTRACT
BACKGROUND: Intramyocellular lipids are an important source of fuel for mitochondrial fat oxidation and play an important role in intramuscular lipid homeostasis. We hypothesised that due to the phenotype associated with cancer cachexia, there would exist an association between increasing weight loss and the number/size of intramyocellular lipid droplets. METHODS: Nineteen cancer patients and 6 controls undergoing surgery were recruited. A rectus abdominis biopsy was performed and processed for transmission electron microscopy (TEM). The number of intramyocellular lipid droplets and lipid droplet diameter were calculated from the TEM images. CT scans, performed as part of patients' routine care, were analysed to determine amount of adipose (intermuscular, visceral and subcutaneous) and muscle tissue. RESULTS: Compared with controls, cancer patients had increased numbers of lipid droplets (mean (SD) 1.8 (1.9) vs. 6.4 (9.1) per ×2,650 field, respectively, p = 0.036). Mean (SD) lipid droplet diameter was also higher in cancer patients compared with controls (0.42 (0.13) vs. 0.24 (0.21) μm, p = 0.015). Mean lipid droplet count correlated positively with the severity of weight loss (R = 0.51, p = 0.025) and negatively with CT-derived measures of intermuscular fat (R = -0.53, p = 0.022) and visceral fat (R = -0.51, p = 0.029). CONCLUSIONS: This study suggests that the number and size of intramyocellular lipid droplets is increased in the presence of cancer and increases further with weight loss/loss of adipose mass in other body compartments.

No MeSH data available.


Related in: MedlinePlus