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Time course of collagen peak in bile duct-ligated rats.

Tarcin O, Basaranoglu M, Tahan V, Tahan G, Sücüllü I, Yilmaz N, Sood G, Snyder N, Hilman G, Celikel C, Tözün N - BMC Gastroenterol (2011)

Bottom Line: Bile duct ligation (BDL) had been performed in the rest.The peak level of collagen was observed biochemically and histomorphometricly at the end of third week (P < 0.001 and P < 0.05).Suprisingly, collagen levels had decreased with the course of time such as at the end of fourth week (P < 0.01 and P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Acibadem Hospital, Istanbul 34500, Turkey.

ABSTRACT

Background: One of the most useful experimental fibrogenesis models is the "bile duct-ligated rats". Our aim was to investigate the quantitative hepatic collagen content by two different methods during the different stages of hepatic fibrosis in bile duct-ligated rats on a weekly basis. We questioned whether the 1-wk or 4-wk bile duct-ligated model is suitable in animal fibrogenesis trials.

Methods: Of the 53 male Wistar rats, 8 (Group 0) were used as a healthy control group. Bile duct ligation (BDL) had been performed in the rest. Bile duct-ligated rates were sacrificed 7 days later in group 1 (10 rats), 14 days later in group 2 (9 rats), 21 days later in group 3(9 rats) and 28 days later in group 4 (9 rats). Eight rats underwent sham-operation (Sham). Hepatic collagen measurements as well as serum levels of liver enzymes and function tests were all analysed.

Results: The peak level of collagen was observed biochemically and histomorphometricly at the end of third week (P < 0.001 and P < 0.05). Suprisingly, collagen levels had decreased with the course of time such as at the end of fourth week (P < 0.01 and P < 0.05).

Conclusion: We have shown that fibrosis in bile duct-ligated rats is transient, i.e. reverses spontaneously after 3 weeks. This contrasts any situation in patients where hepatic fibrosis is progressive and irreversible as countless studies performed by many investigators in the same animal model.

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There was a significant increase in hepatic collagen amount in BDL groups histomorphometricaly compared to the control and sham groups (P < 0.01). Collagen peak was found at the end of the third week when compared to the groups 1, 2 and 4 (P < 0.05).
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Figure 4: There was a significant increase in hepatic collagen amount in BDL groups histomorphometricaly compared to the control and sham groups (P < 0.01). Collagen peak was found at the end of the third week when compared to the groups 1, 2 and 4 (P < 0.05).

Mentions: Histomorhometric measurement for collagen demonstrated a very similar pattern as the biochemical collagen measurement. We found that the peak level of collagen was at the third week and a decrease at the fourth week (P < 0.05) (Figure 4).


Time course of collagen peak in bile duct-ligated rats.

Tarcin O, Basaranoglu M, Tahan V, Tahan G, Sücüllü I, Yilmaz N, Sood G, Snyder N, Hilman G, Celikel C, Tözün N - BMC Gastroenterol (2011)

There was a significant increase in hepatic collagen amount in BDL groups histomorphometricaly compared to the control and sham groups (P < 0.01). Collagen peak was found at the end of the third week when compared to the groups 1, 2 and 4 (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3117813&req=5

Figure 4: There was a significant increase in hepatic collagen amount in BDL groups histomorphometricaly compared to the control and sham groups (P < 0.01). Collagen peak was found at the end of the third week when compared to the groups 1, 2 and 4 (P < 0.05).
Mentions: Histomorhometric measurement for collagen demonstrated a very similar pattern as the biochemical collagen measurement. We found that the peak level of collagen was at the third week and a decrease at the fourth week (P < 0.05) (Figure 4).

Bottom Line: Bile duct ligation (BDL) had been performed in the rest.The peak level of collagen was observed biochemically and histomorphometricly at the end of third week (P < 0.001 and P < 0.05).Suprisingly, collagen levels had decreased with the course of time such as at the end of fourth week (P < 0.01 and P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Acibadem Hospital, Istanbul 34500, Turkey.

ABSTRACT

Background: One of the most useful experimental fibrogenesis models is the "bile duct-ligated rats". Our aim was to investigate the quantitative hepatic collagen content by two different methods during the different stages of hepatic fibrosis in bile duct-ligated rats on a weekly basis. We questioned whether the 1-wk or 4-wk bile duct-ligated model is suitable in animal fibrogenesis trials.

Methods: Of the 53 male Wistar rats, 8 (Group 0) were used as a healthy control group. Bile duct ligation (BDL) had been performed in the rest. Bile duct-ligated rates were sacrificed 7 days later in group 1 (10 rats), 14 days later in group 2 (9 rats), 21 days later in group 3(9 rats) and 28 days later in group 4 (9 rats). Eight rats underwent sham-operation (Sham). Hepatic collagen measurements as well as serum levels of liver enzymes and function tests were all analysed.

Results: The peak level of collagen was observed biochemically and histomorphometricly at the end of third week (P < 0.001 and P < 0.05). Suprisingly, collagen levels had decreased with the course of time such as at the end of fourth week (P < 0.01 and P < 0.05).

Conclusion: We have shown that fibrosis in bile duct-ligated rats is transient, i.e. reverses spontaneously after 3 weeks. This contrasts any situation in patients where hepatic fibrosis is progressive and irreversible as countless studies performed by many investigators in the same animal model.

Show MeSH
Related in: MedlinePlus