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Time course of collagen peak in bile duct-ligated rats.

Tarcin O, Basaranoglu M, Tahan V, Tahan G, Sücüllü I, Yilmaz N, Sood G, Snyder N, Hilman G, Celikel C, Tözün N - BMC Gastroenterol (2011)

Bottom Line: Hepatic collagen measurements as well as serum levels of liver enzymes and function tests were all analysed.The peak level of collagen was observed biochemically and histomorphometricly at the end of third week (P < 0.001 and P < 0.05).Suprisingly, collagen levels had decreased with the course of time such as at the end of fourth week (P < 0.01 and P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Acibadem Hospital, Istanbul 34500, Turkey.

ABSTRACT

Background: One of the most useful experimental fibrogenesis models is the "bile duct-ligated rats". Our aim was to investigate the quantitative hepatic collagen content by two different methods during the different stages of hepatic fibrosis in bile duct-ligated rats on a weekly basis. We questioned whether the 1-wk or 4-wk bile duct-ligated model is suitable in animal fibrogenesis trials.

Methods: Of the 53 male Wistar rats, 8 (Group 0) were used as a healthy control group. Bile duct ligation (BDL) had been performed in the rest. Bile duct-ligated rates were sacrificed 7 days later in group 1 (10 rats), 14 days later in group 2 (9 rats), 21 days later in group 3(9 rats) and 28 days later in group 4 (9 rats). Eight rats underwent sham-operation (Sham). Hepatic collagen measurements as well as serum levels of liver enzymes and function tests were all analysed.

Results: The peak level of collagen was observed biochemically and histomorphometricly at the end of third week (P < 0.001 and P < 0.05). Suprisingly, collagen levels had decreased with the course of time such as at the end of fourth week (P < 0.01 and P < 0.05).

Conclusion: We have shown that fibrosis in bile duct-ligated rats is transient, i.e. reverses spontaneously after 3 weeks. This contrasts any situation in patients where hepatic fibrosis is progressive and irreversible as countless studies performed by many investigators in the same animal model.

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Related in: MedlinePlus

There was a significant increase in hepatic collagen amount in BDL groups histomorphometricaly compared to the control and sham groups (P < 0.01). Collagen peak was found at the end of the third week when compared to the groups 1, 2 and 4 (P < 0.05).
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Figure 4: There was a significant increase in hepatic collagen amount in BDL groups histomorphometricaly compared to the control and sham groups (P < 0.01). Collagen peak was found at the end of the third week when compared to the groups 1, 2 and 4 (P < 0.05).

Mentions: Histomorhometric measurement for collagen demonstrated a very similar pattern as the biochemical collagen measurement. We found that the peak level of collagen was at the third week and a decrease at the fourth week (P < 0.05) (Figure 4).


Time course of collagen peak in bile duct-ligated rats.

Tarcin O, Basaranoglu M, Tahan V, Tahan G, Sücüllü I, Yilmaz N, Sood G, Snyder N, Hilman G, Celikel C, Tözün N - BMC Gastroenterol (2011)

There was a significant increase in hepatic collagen amount in BDL groups histomorphometricaly compared to the control and sham groups (P < 0.01). Collagen peak was found at the end of the third week when compared to the groups 1, 2 and 4 (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3117813&req=5

Figure 4: There was a significant increase in hepatic collagen amount in BDL groups histomorphometricaly compared to the control and sham groups (P < 0.01). Collagen peak was found at the end of the third week when compared to the groups 1, 2 and 4 (P < 0.05).
Mentions: Histomorhometric measurement for collagen demonstrated a very similar pattern as the biochemical collagen measurement. We found that the peak level of collagen was at the third week and a decrease at the fourth week (P < 0.05) (Figure 4).

Bottom Line: Hepatic collagen measurements as well as serum levels of liver enzymes and function tests were all analysed.The peak level of collagen was observed biochemically and histomorphometricly at the end of third week (P < 0.001 and P < 0.05).Suprisingly, collagen levels had decreased with the course of time such as at the end of fourth week (P < 0.01 and P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Acibadem Hospital, Istanbul 34500, Turkey.

ABSTRACT

Background: One of the most useful experimental fibrogenesis models is the "bile duct-ligated rats". Our aim was to investigate the quantitative hepatic collagen content by two different methods during the different stages of hepatic fibrosis in bile duct-ligated rats on a weekly basis. We questioned whether the 1-wk or 4-wk bile duct-ligated model is suitable in animal fibrogenesis trials.

Methods: Of the 53 male Wistar rats, 8 (Group 0) were used as a healthy control group. Bile duct ligation (BDL) had been performed in the rest. Bile duct-ligated rates were sacrificed 7 days later in group 1 (10 rats), 14 days later in group 2 (9 rats), 21 days later in group 3(9 rats) and 28 days later in group 4 (9 rats). Eight rats underwent sham-operation (Sham). Hepatic collagen measurements as well as serum levels of liver enzymes and function tests were all analysed.

Results: The peak level of collagen was observed biochemically and histomorphometricly at the end of third week (P < 0.001 and P < 0.05). Suprisingly, collagen levels had decreased with the course of time such as at the end of fourth week (P < 0.01 and P < 0.05).

Conclusion: We have shown that fibrosis in bile duct-ligated rats is transient, i.e. reverses spontaneously after 3 weeks. This contrasts any situation in patients where hepatic fibrosis is progressive and irreversible as countless studies performed by many investigators in the same animal model.

Show MeSH
Related in: MedlinePlus