Limits...
Increased IL-10 mRNA expression in tumor-associated macrophage correlated with late stage of lung cancer.

Wang R, Lu M, Zhang J, Chen S, Luo X, Qin Y, Chen H - J. Exp. Clin. Cancer Res. (2011)

Bottom Line: We successfully achieved up to 95% purity of TAM, derived from 63 primary lung cancer tissues.Our results revealed that TAM with high levels of IL-10 expression may play an important role in the progression of non-small cell lung cancer.Additionally, the phenotype of isolated TAM can be potentially used to predict clinicopathological features as well.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Thoracic Surgery, Shanghai Cancer Hospital, Fudan University, China.

ABSTRACT

Background: Monocyte recruited into the tumor and maturation to tumor-associated macrophage (TAM). Interleukin-10(IL-10) is a potent immunosuppressive cytokine, which can be secreted from both primary tumor and stromal cells. However, there are controversies regarding its role in the progression of cancer. So it is important to isolate TAM from tumor cells to study the role of IL-10 in the progress of cancer. The aim of our study was to determine whether IL-10 expressed by TAM correlated with clinicopathological factors in NSCLC.

Methods: TAM in NSCLC was isolated by short-term culture in serum free medium with the modification to literature reports. The mRNA expression levels of IL-10, cathepsin B, cathepsin S, which were closely related with TAM according to the literatures, were evaluated by Quantitative real-time RT-PCR in 63 NSCLC. The relationships between their expression levels and clinicopathological features were investigated.

Results: We successfully achieved up to 95% purity of TAM, derived from 63 primary lung cancer tissues. TAM expressed high levels of IL-10, cathepsin B in NSCLC. High levels of IL-10 in TAM significantly correlated with stage, tumor size, lymph node metastasis, lymphovascular invasion or histologic poor differentiation.

Conclusions: Our results revealed that TAM with high levels of IL-10 expression may play an important role in the progression of non-small cell lung cancer. The data also suggested that TAMs may involve in tumor immunosuppression through overexpressed IL-10. Additionally, the phenotype of isolated TAM can be potentially used to predict clinicopathological features as well.

Show MeSH

Related in: MedlinePlus

mRNA from TAMs and matched normal macrophage(Mφ) was analyzed by Quantitative real-time RT-PCR for expression of the indicated genes in 63 NSCLC samples. Results are given as fold increase in mRNA expression with respect to expression in matched Mφ. Data were normalized to expression of the β-actin gene. Mφ was used as a calibrator. Bars represent median. *p by the Mann-Whitney U test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3117740&req=5

Figure 4: mRNA from TAMs and matched normal macrophage(Mφ) was analyzed by Quantitative real-time RT-PCR for expression of the indicated genes in 63 NSCLC samples. Results are given as fold increase in mRNA expression with respect to expression in matched Mφ. Data were normalized to expression of the β-actin gene. Mφ was used as a calibrator. Bars represent median. *p by the Mann-Whitney U test.

Mentions: Compared with the expression in macrophage, IL-10 and cathepsin B were significantly upregulated (p < 0.05). After normalized to macrophages, the median values (range) of each gene in TAM were: IL-10, 30.5(0.6-530.3) and cathepsin B, 11.9(0.6-69.1) (Figure 4 A-B). There were no significant differences in the level of cathepsin S between the TAMs(0.85(0.04-4.49))and the macrophages (Figure 4C).


Increased IL-10 mRNA expression in tumor-associated macrophage correlated with late stage of lung cancer.

Wang R, Lu M, Zhang J, Chen S, Luo X, Qin Y, Chen H - J. Exp. Clin. Cancer Res. (2011)

mRNA from TAMs and matched normal macrophage(Mφ) was analyzed by Quantitative real-time RT-PCR for expression of the indicated genes in 63 NSCLC samples. Results are given as fold increase in mRNA expression with respect to expression in matched Mφ. Data were normalized to expression of the β-actin gene. Mφ was used as a calibrator. Bars represent median. *p by the Mann-Whitney U test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3117740&req=5

Figure 4: mRNA from TAMs and matched normal macrophage(Mφ) was analyzed by Quantitative real-time RT-PCR for expression of the indicated genes in 63 NSCLC samples. Results are given as fold increase in mRNA expression with respect to expression in matched Mφ. Data were normalized to expression of the β-actin gene. Mφ was used as a calibrator. Bars represent median. *p by the Mann-Whitney U test.
Mentions: Compared with the expression in macrophage, IL-10 and cathepsin B were significantly upregulated (p < 0.05). After normalized to macrophages, the median values (range) of each gene in TAM were: IL-10, 30.5(0.6-530.3) and cathepsin B, 11.9(0.6-69.1) (Figure 4 A-B). There were no significant differences in the level of cathepsin S between the TAMs(0.85(0.04-4.49))and the macrophages (Figure 4C).

Bottom Line: We successfully achieved up to 95% purity of TAM, derived from 63 primary lung cancer tissues.Our results revealed that TAM with high levels of IL-10 expression may play an important role in the progression of non-small cell lung cancer.Additionally, the phenotype of isolated TAM can be potentially used to predict clinicopathological features as well.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Thoracic Surgery, Shanghai Cancer Hospital, Fudan University, China.

ABSTRACT

Background: Monocyte recruited into the tumor and maturation to tumor-associated macrophage (TAM). Interleukin-10(IL-10) is a potent immunosuppressive cytokine, which can be secreted from both primary tumor and stromal cells. However, there are controversies regarding its role in the progression of cancer. So it is important to isolate TAM from tumor cells to study the role of IL-10 in the progress of cancer. The aim of our study was to determine whether IL-10 expressed by TAM correlated with clinicopathological factors in NSCLC.

Methods: TAM in NSCLC was isolated by short-term culture in serum free medium with the modification to literature reports. The mRNA expression levels of IL-10, cathepsin B, cathepsin S, which were closely related with TAM according to the literatures, were evaluated by Quantitative real-time RT-PCR in 63 NSCLC. The relationships between their expression levels and clinicopathological features were investigated.

Results: We successfully achieved up to 95% purity of TAM, derived from 63 primary lung cancer tissues. TAM expressed high levels of IL-10, cathepsin B in NSCLC. High levels of IL-10 in TAM significantly correlated with stage, tumor size, lymph node metastasis, lymphovascular invasion or histologic poor differentiation.

Conclusions: Our results revealed that TAM with high levels of IL-10 expression may play an important role in the progression of non-small cell lung cancer. The data also suggested that TAMs may involve in tumor immunosuppression through overexpressed IL-10. Additionally, the phenotype of isolated TAM can be potentially used to predict clinicopathological features as well.

Show MeSH
Related in: MedlinePlus