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Vascular endothelial growth factor-A (VEGF-A) and chemokine ligand-2 (CCL2) in amyotrophic lateral sclerosis (ALS) patients.

Gupta PK, Prabhakar S, Sharma S, Anand A - J Neuroinflammation (2011)

Bottom Line: Their levels were adjusted for possible confounders like cigarette smoking, alcohol and meat consumption.Contrary to previous studies, VEGF-A was found to be elevated significantly in serum and CSF in ALS patient population studied.We also found an increase in CCL2 levels in CSF of these ALS patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neurology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh-160012, India.

ABSTRACT

Background: Vascular endothelial growth factor-A (VEGF-A) and chemokne ligand-2 (CCL2) levels have been examined in Amyotrophic Lateral Sclerosis (ALS) patients in Western countries. We measured these values in North Indian ALS patients, since these patients display considerably enhanced survival duration.

Methods: Sporadic ALS patients were included on the basis of El Escorial criteria. VEGF-A and CCL2 levels were analyzed in serum and cerebrospinal fluid (CSF) of 50 ALS patients using enzyme linked immunosorbent assay (ELISA) and compared with normal controls. Their levels were adjusted for possible confounders like cigarette smoking, alcohol and meat consumption.

Results: Contrary to previous studies, VEGF-A was found to be elevated significantly in serum and CSF in ALS patient population studied. We also found an increase in CCL2 levels in CSF of these ALS patients. Serum and CSF from definite ALS revealed higher VEGF-A as compared to probable and possible ALS. CCL2 was unaltered between definite, probable and possible ALS. Univariate and multivariate analysis revealed a lack of association of smoking, alcohol and meat consumption with VEGF-A and CCL2 levels.

Conclusions: VEGF-A upregulation may indicate an activation of compensatory responses in ALS which may reflect or in fact account for increased survival of North Indian ALS patients after disease onset. The intrathecal synthesis of CCL2 suggests the involvement of adult neural stem cells and microglial activation in ALS pathogenesis which needs further investigation.

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Related in: MedlinePlus

Level of VEGF-A in serum (A) and CSF (B) of ALS patients with respiratory dysfunction. Boxes include values from first quartile (25th percentile) to third quartile (75th percentile). Lower and upper error bar refers to 10th and 90th percentile respectively. The thick horizontal line in the box represents median for each dataset. Levels of VEGF-A were normalized to total serum and CSF protein respectively. Outliers are shown in circles. # indicates significant difference (p < 0.05) between the given conditions. Data was analyzed by Mann Whitney U Test. ALS, Amyotrophic Lateral Sclerosis; VEGF-A, vascular endothelial growth factor-A; pg, picogram; μg, microgram.
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Figure 5: Level of VEGF-A in serum (A) and CSF (B) of ALS patients with respiratory dysfunction. Boxes include values from first quartile (25th percentile) to third quartile (75th percentile). Lower and upper error bar refers to 10th and 90th percentile respectively. The thick horizontal line in the box represents median for each dataset. Levels of VEGF-A were normalized to total serum and CSF protein respectively. Outliers are shown in circles. # indicates significant difference (p < 0.05) between the given conditions. Data was analyzed by Mann Whitney U Test. ALS, Amyotrophic Lateral Sclerosis; VEGF-A, vascular endothelial growth factor-A; pg, picogram; μg, microgram.

Mentions: Further analysis shows significantly increased VEGF-A in 11 ALS patients with respiratory dysfunction indicating a possible association with hypoxia (Figure 5A-5B; Table 3). Elevated VEGF-A in serum and CSF of definite ALS suggests extensive involvement of neuroaxis and relatively higher degree of neurodegeneration and regeneration than probable and possible variants. No significant difference in median disease duration between definite [14(4-36) months], probable [16(3-72) months] and possible ALS [14(9-24) months] cases was observed. It must be pointed out that while the disease duration was accurately documented, the actual survival time of patients after disease onset could not be ascertained.


Vascular endothelial growth factor-A (VEGF-A) and chemokine ligand-2 (CCL2) in amyotrophic lateral sclerosis (ALS) patients.

Gupta PK, Prabhakar S, Sharma S, Anand A - J Neuroinflammation (2011)

Level of VEGF-A in serum (A) and CSF (B) of ALS patients with respiratory dysfunction. Boxes include values from first quartile (25th percentile) to third quartile (75th percentile). Lower and upper error bar refers to 10th and 90th percentile respectively. The thick horizontal line in the box represents median for each dataset. Levels of VEGF-A were normalized to total serum and CSF protein respectively. Outliers are shown in circles. # indicates significant difference (p < 0.05) between the given conditions. Data was analyzed by Mann Whitney U Test. ALS, Amyotrophic Lateral Sclerosis; VEGF-A, vascular endothelial growth factor-A; pg, picogram; μg, microgram.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3117705&req=5

Figure 5: Level of VEGF-A in serum (A) and CSF (B) of ALS patients with respiratory dysfunction. Boxes include values from first quartile (25th percentile) to third quartile (75th percentile). Lower and upper error bar refers to 10th and 90th percentile respectively. The thick horizontal line in the box represents median for each dataset. Levels of VEGF-A were normalized to total serum and CSF protein respectively. Outliers are shown in circles. # indicates significant difference (p < 0.05) between the given conditions. Data was analyzed by Mann Whitney U Test. ALS, Amyotrophic Lateral Sclerosis; VEGF-A, vascular endothelial growth factor-A; pg, picogram; μg, microgram.
Mentions: Further analysis shows significantly increased VEGF-A in 11 ALS patients with respiratory dysfunction indicating a possible association with hypoxia (Figure 5A-5B; Table 3). Elevated VEGF-A in serum and CSF of definite ALS suggests extensive involvement of neuroaxis and relatively higher degree of neurodegeneration and regeneration than probable and possible variants. No significant difference in median disease duration between definite [14(4-36) months], probable [16(3-72) months] and possible ALS [14(9-24) months] cases was observed. It must be pointed out that while the disease duration was accurately documented, the actual survival time of patients after disease onset could not be ascertained.

Bottom Line: Their levels were adjusted for possible confounders like cigarette smoking, alcohol and meat consumption.Contrary to previous studies, VEGF-A was found to be elevated significantly in serum and CSF in ALS patient population studied.We also found an increase in CCL2 levels in CSF of these ALS patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neurology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh-160012, India.

ABSTRACT

Background: Vascular endothelial growth factor-A (VEGF-A) and chemokne ligand-2 (CCL2) levels have been examined in Amyotrophic Lateral Sclerosis (ALS) patients in Western countries. We measured these values in North Indian ALS patients, since these patients display considerably enhanced survival duration.

Methods: Sporadic ALS patients were included on the basis of El Escorial criteria. VEGF-A and CCL2 levels were analyzed in serum and cerebrospinal fluid (CSF) of 50 ALS patients using enzyme linked immunosorbent assay (ELISA) and compared with normal controls. Their levels were adjusted for possible confounders like cigarette smoking, alcohol and meat consumption.

Results: Contrary to previous studies, VEGF-A was found to be elevated significantly in serum and CSF in ALS patient population studied. We also found an increase in CCL2 levels in CSF of these ALS patients. Serum and CSF from definite ALS revealed higher VEGF-A as compared to probable and possible ALS. CCL2 was unaltered between definite, probable and possible ALS. Univariate and multivariate analysis revealed a lack of association of smoking, alcohol and meat consumption with VEGF-A and CCL2 levels.

Conclusions: VEGF-A upregulation may indicate an activation of compensatory responses in ALS which may reflect or in fact account for increased survival of North Indian ALS patients after disease onset. The intrathecal synthesis of CCL2 suggests the involvement of adult neural stem cells and microglial activation in ALS pathogenesis which needs further investigation.

Show MeSH
Related in: MedlinePlus