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GhMPK16, a novel stress-responsive group D MAPK gene from cotton, is involved in disease resistance and drought sensitivity.

Shi J, Zhang L, An H, Wu C, Guo X - BMC Mol. Biol. (2011)

Bottom Line: In this study, we isolated and characterised GhMPK16, the first group D MAPK gene found in cotton.Furthermore, transgenic Arabidopsis showed reduced drought tolerance and rapid H2O2 accumulation.These results suggest that GhMPK16 might be involved in multiple signal transduction pathways, including biotic and abiotic stress signaling pathways.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong 271018, China.

ABSTRACT

Background: Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in mediating biotic and abiotic stress responses. In plants, MAPKs are classified into four major groups (A-D) according to their sequence homology and conserved phosphorylation motifs. Members of group A and B have been extensively characterized, but little information on the group D MAPKs has been reported.

Results: In this study, we isolated and characterised GhMPK16, the first group D MAPK gene found in cotton. Southern blot analysis suggests GhMPK16 is single copy in the cotton genome, and RNA blot analysis indicates that GhMPK16 transcripts accumulate following pathogen infection and treatment with multiple defense-related signal molecules. The analysis of the promoter region of GhMPK16 revealed a group of putative cis-acting elements related to stress responses. Subcellular localization analysis suggests that GhMPK16 acts in the nucleus. Transgenic Arabidopsis overexpressing GhMPK16 displayed significant resistance to fungi (Colletotrichum nicotianae and Alternaria alternata) and bacteria (Pseudomonas solanacearum) pathogen, and the transcripts of pathogen-related (PR) genes were more rapidly and strongly induced in the transgenic plants. Furthermore, transgenic Arabidopsis showed reduced drought tolerance and rapid H2O2 accumulation.

Conclusion: These results suggest that GhMPK16 might be involved in multiple signal transduction pathways, including biotic and abiotic stress signaling pathways.

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Related in: MedlinePlus

Nucleotide sequence of the promoter region of GhMPK16. The predicted transcription initiation site is indicated (+1, A). The start codon is marked with an asterisk, and the putative core promoter consensus sequences (TATA-box and CAAT-box) are highlighted in grey. The putative cis-acting elements are indicated by boxes and their corresponding names are given above each element. Arrows indicate the direction of the cis-element. Box-W1 is a fungal elicitor responsive element, ERE is an ethylene-responsive element, the MBS binding site is involved in gene induction in response to drought-inducibility and the TCA-element is a cis-acting element involved in SA-responsive.
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Figure 5: Nucleotide sequence of the promoter region of GhMPK16. The predicted transcription initiation site is indicated (+1, A). The start codon is marked with an asterisk, and the putative core promoter consensus sequences (TATA-box and CAAT-box) are highlighted in grey. The putative cis-acting elements are indicated by boxes and their corresponding names are given above each element. Arrows indicate the direction of the cis-element. Box-W1 is a fungal elicitor responsive element, ERE is an ethylene-responsive element, the MBS binding site is involved in gene induction in response to drought-inducibility and the TCA-element is a cis-acting element involved in SA-responsive.

Mentions: Inverse-PCR (I-PCR) was used to obtain a 785 bp fragment of the 5' flanking region upstream of the transcriptional start site, as determined by the GhMPK16 cDNA sequence. In order to find cis-acting elements, the PlantCARE databases were analyzed. Sequence analysis revealed that the GhMPK16 promoter contains TATA and CAAT motifs located at nucleotides -38 and -64 relative to the transcriptional start site, respectively, which is characteristic of eukaryotic gene promoters. As shown in Figure 5, the 785 bp promoter region contains several motifs probably related to pathogen and drought signals, such as a Box-W1 element (fungal elicitor responsive element), an ERE element (ethylene-responsive element), two TCA-element (cis-acting SA-responsive element) and a MBS element (MYB binding site involved in drought-inducibility). This suggests that these putative cis-acting elements are responsible for enhanced expression of GhMPK16 during stress conditions.


GhMPK16, a novel stress-responsive group D MAPK gene from cotton, is involved in disease resistance and drought sensitivity.

Shi J, Zhang L, An H, Wu C, Guo X - BMC Mol. Biol. (2011)

Nucleotide sequence of the promoter region of GhMPK16. The predicted transcription initiation site is indicated (+1, A). The start codon is marked with an asterisk, and the putative core promoter consensus sequences (TATA-box and CAAT-box) are highlighted in grey. The putative cis-acting elements are indicated by boxes and their corresponding names are given above each element. Arrows indicate the direction of the cis-element. Box-W1 is a fungal elicitor responsive element, ERE is an ethylene-responsive element, the MBS binding site is involved in gene induction in response to drought-inducibility and the TCA-element is a cis-acting element involved in SA-responsive.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3117701&req=5

Figure 5: Nucleotide sequence of the promoter region of GhMPK16. The predicted transcription initiation site is indicated (+1, A). The start codon is marked with an asterisk, and the putative core promoter consensus sequences (TATA-box and CAAT-box) are highlighted in grey. The putative cis-acting elements are indicated by boxes and their corresponding names are given above each element. Arrows indicate the direction of the cis-element. Box-W1 is a fungal elicitor responsive element, ERE is an ethylene-responsive element, the MBS binding site is involved in gene induction in response to drought-inducibility and the TCA-element is a cis-acting element involved in SA-responsive.
Mentions: Inverse-PCR (I-PCR) was used to obtain a 785 bp fragment of the 5' flanking region upstream of the transcriptional start site, as determined by the GhMPK16 cDNA sequence. In order to find cis-acting elements, the PlantCARE databases were analyzed. Sequence analysis revealed that the GhMPK16 promoter contains TATA and CAAT motifs located at nucleotides -38 and -64 relative to the transcriptional start site, respectively, which is characteristic of eukaryotic gene promoters. As shown in Figure 5, the 785 bp promoter region contains several motifs probably related to pathogen and drought signals, such as a Box-W1 element (fungal elicitor responsive element), an ERE element (ethylene-responsive element), two TCA-element (cis-acting SA-responsive element) and a MBS element (MYB binding site involved in drought-inducibility). This suggests that these putative cis-acting elements are responsible for enhanced expression of GhMPK16 during stress conditions.

Bottom Line: In this study, we isolated and characterised GhMPK16, the first group D MAPK gene found in cotton.Furthermore, transgenic Arabidopsis showed reduced drought tolerance and rapid H2O2 accumulation.These results suggest that GhMPK16 might be involved in multiple signal transduction pathways, including biotic and abiotic stress signaling pathways.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong 271018, China.

ABSTRACT

Background: Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in mediating biotic and abiotic stress responses. In plants, MAPKs are classified into four major groups (A-D) according to their sequence homology and conserved phosphorylation motifs. Members of group A and B have been extensively characterized, but little information on the group D MAPKs has been reported.

Results: In this study, we isolated and characterised GhMPK16, the first group D MAPK gene found in cotton. Southern blot analysis suggests GhMPK16 is single copy in the cotton genome, and RNA blot analysis indicates that GhMPK16 transcripts accumulate following pathogen infection and treatment with multiple defense-related signal molecules. The analysis of the promoter region of GhMPK16 revealed a group of putative cis-acting elements related to stress responses. Subcellular localization analysis suggests that GhMPK16 acts in the nucleus. Transgenic Arabidopsis overexpressing GhMPK16 displayed significant resistance to fungi (Colletotrichum nicotianae and Alternaria alternata) and bacteria (Pseudomonas solanacearum) pathogen, and the transcripts of pathogen-related (PR) genes were more rapidly and strongly induced in the transgenic plants. Furthermore, transgenic Arabidopsis showed reduced drought tolerance and rapid H2O2 accumulation.

Conclusion: These results suggest that GhMPK16 might be involved in multiple signal transduction pathways, including biotic and abiotic stress signaling pathways.

Show MeSH
Related in: MedlinePlus