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GhMPK16, a novel stress-responsive group D MAPK gene from cotton, is involved in disease resistance and drought sensitivity.

Shi J, Zhang L, An H, Wu C, Guo X - BMC Mol. Biol. (2011)

Bottom Line: In this study, we isolated and characterised GhMPK16, the first group D MAPK gene found in cotton.Furthermore, transgenic Arabidopsis showed reduced drought tolerance and rapid H2O2 accumulation.These results suggest that GhMPK16 might be involved in multiple signal transduction pathways, including biotic and abiotic stress signaling pathways.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong 271018, China.

ABSTRACT

Background: Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in mediating biotic and abiotic stress responses. In plants, MAPKs are classified into four major groups (A-D) according to their sequence homology and conserved phosphorylation motifs. Members of group A and B have been extensively characterized, but little information on the group D MAPKs has been reported.

Results: In this study, we isolated and characterised GhMPK16, the first group D MAPK gene found in cotton. Southern blot analysis suggests GhMPK16 is single copy in the cotton genome, and RNA blot analysis indicates that GhMPK16 transcripts accumulate following pathogen infection and treatment with multiple defense-related signal molecules. The analysis of the promoter region of GhMPK16 revealed a group of putative cis-acting elements related to stress responses. Subcellular localization analysis suggests that GhMPK16 acts in the nucleus. Transgenic Arabidopsis overexpressing GhMPK16 displayed significant resistance to fungi (Colletotrichum nicotianae and Alternaria alternata) and bacteria (Pseudomonas solanacearum) pathogen, and the transcripts of pathogen-related (PR) genes were more rapidly and strongly induced in the transgenic plants. Furthermore, transgenic Arabidopsis showed reduced drought tolerance and rapid H2O2 accumulation.

Conclusion: These results suggest that GhMPK16 might be involved in multiple signal transduction pathways, including biotic and abiotic stress signaling pathways.

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Southern blot analysis for GhMPK16 in the cotton genome. Genomic DNA (30 mg/sample) was digested with EcoR V, Xba I, Hind III and EcoR I respectively, followed by hybridization with the partial α-32P -labeled genomic GhMPK16 fragment. Molecular weight marker is shown on the right.
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Figure 2: Southern blot analysis for GhMPK16 in the cotton genome. Genomic DNA (30 mg/sample) was digested with EcoR V, Xba I, Hind III and EcoR I respectively, followed by hybridization with the partial α-32P -labeled genomic GhMPK16 fragment. Molecular weight marker is shown on the right.

Mentions: Southern blots were used to investigate the genomic organization of the GhMPK16 gene. Genomic DNA was completely digested with EcoR V, Xba I, Hind III and EcoR I and hybridised to the 3' partial sequence of GhMPK16, which contains no restriction sites of EcoR V, Hind III and EcoR I and only one Xba I site presents in the probe region. As shown in Figure 2, only one band was observed for the EcoR V, Hind III and EcoR I digestions, and two bands were observed for the Xba I digestion. These results imply that there is a single GhMPK16 gene in the cotton genome.


GhMPK16, a novel stress-responsive group D MAPK gene from cotton, is involved in disease resistance and drought sensitivity.

Shi J, Zhang L, An H, Wu C, Guo X - BMC Mol. Biol. (2011)

Southern blot analysis for GhMPK16 in the cotton genome. Genomic DNA (30 mg/sample) was digested with EcoR V, Xba I, Hind III and EcoR I respectively, followed by hybridization with the partial α-32P -labeled genomic GhMPK16 fragment. Molecular weight marker is shown on the right.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3117701&req=5

Figure 2: Southern blot analysis for GhMPK16 in the cotton genome. Genomic DNA (30 mg/sample) was digested with EcoR V, Xba I, Hind III and EcoR I respectively, followed by hybridization with the partial α-32P -labeled genomic GhMPK16 fragment. Molecular weight marker is shown on the right.
Mentions: Southern blots were used to investigate the genomic organization of the GhMPK16 gene. Genomic DNA was completely digested with EcoR V, Xba I, Hind III and EcoR I and hybridised to the 3' partial sequence of GhMPK16, which contains no restriction sites of EcoR V, Hind III and EcoR I and only one Xba I site presents in the probe region. As shown in Figure 2, only one band was observed for the EcoR V, Hind III and EcoR I digestions, and two bands were observed for the Xba I digestion. These results imply that there is a single GhMPK16 gene in the cotton genome.

Bottom Line: In this study, we isolated and characterised GhMPK16, the first group D MAPK gene found in cotton.Furthermore, transgenic Arabidopsis showed reduced drought tolerance and rapid H2O2 accumulation.These results suggest that GhMPK16 might be involved in multiple signal transduction pathways, including biotic and abiotic stress signaling pathways.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong 271018, China.

ABSTRACT

Background: Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in mediating biotic and abiotic stress responses. In plants, MAPKs are classified into four major groups (A-D) according to their sequence homology and conserved phosphorylation motifs. Members of group A and B have been extensively characterized, but little information on the group D MAPKs has been reported.

Results: In this study, we isolated and characterised GhMPK16, the first group D MAPK gene found in cotton. Southern blot analysis suggests GhMPK16 is single copy in the cotton genome, and RNA blot analysis indicates that GhMPK16 transcripts accumulate following pathogen infection and treatment with multiple defense-related signal molecules. The analysis of the promoter region of GhMPK16 revealed a group of putative cis-acting elements related to stress responses. Subcellular localization analysis suggests that GhMPK16 acts in the nucleus. Transgenic Arabidopsis overexpressing GhMPK16 displayed significant resistance to fungi (Colletotrichum nicotianae and Alternaria alternata) and bacteria (Pseudomonas solanacearum) pathogen, and the transcripts of pathogen-related (PR) genes were more rapidly and strongly induced in the transgenic plants. Furthermore, transgenic Arabidopsis showed reduced drought tolerance and rapid H2O2 accumulation.

Conclusion: These results suggest that GhMPK16 might be involved in multiple signal transduction pathways, including biotic and abiotic stress signaling pathways.

Show MeSH
Related in: MedlinePlus