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Evaluation of anticataract potential of Triphala in selenite-induced cataract: In vitro and in vivo studies.

Gupta SK, Kalaiselvan V, Srivastava S, Agrawal SS, Saxena R - J Ayurveda Integr Med (2010)

Bottom Line: TP significantly (P < 0.01) restored GSH and decreased malondialdehyde levels.A significant restoration in the activities of antioxidant enzymes such as superoxide dismutase (P < 0.05), catalase (P < 0.05), glutathione peroxidase (P < 0.05), and glutathione-s-transferase (P < 0.005) was observed in the TP-supplemented group compared to controls.This effect may be due to antioxidant activity.

View Article: PubMed Central - PubMed

Affiliation: Delhi Institute of Pharmaceutical Sciences and Research, New Delhi, India.

ABSTRACT
Triphala (TP) is composed of Emblica officinalis, Terminalia chebula, and Terminalia belerica. The present study was undertaken to evaluate its anticataract potential in vitro and in vivo in a selenite-induced experimental model of cataract. In vitro enucleated rat lenses were maintained in organ culture containing Dulbecco's Modified Eagles Medium alone or with the addition of 100μM selenite. These served as the normal and control groups, respectively. In the test group, the medium was supplemented with selenite and different concentrations of TP aqueous extract. The lenses were incubated for 24 h at 37°C. After incubation, the lenses were processed to estimate reduced glutathione (GSH), lipid peroxidation product, and antioxidant enzymes. In vivo selenite cataract was induced in 9-day-old rat pups by subcutaneous injection of sodium selenite (25 μmole/kg body weight). The test groups received 25, 50, and 75 mg/kg of TP intraperitoneally 4 h before the selenite challenge. At the end of the study period, the rats' eyes were examined by slit-lamp. TP significantly (P < 0.01) restored GSH and decreased malondialdehyde levels. A significant restoration in the activities of antioxidant enzymes such as superoxide dismutase (P < 0.05), catalase (P < 0.05), glutathione peroxidase (P < 0.05), and glutathione-s-transferase (P < 0.005) was observed in the TP-supplemented group compared to controls. In vivo TF 25mg/kg developed only 20% nuclear cataract as compared to 100% in control. TP prevents or retards experimental selenite-induced cataract. This effect may be due to antioxidant activity. Further studies are warranted to explore its role in human cataract.

No MeSH data available.


Related in: MedlinePlus

Effect of Triphala on MDA levels in selenite-induced oxidative stress in vitro, Normal: DMEM, Control: DMEM+100μM of Sodium selenite, Treated: DMEM+100μM of Sodium selenite + Triphala. Incubation period 24 h. Values are mean±SD. *P<0.01 and #P <0.05 as compared to control. n=6.
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Figure 0004: Effect of Triphala on MDA levels in selenite-induced oxidative stress in vitro, Normal: DMEM, Control: DMEM+100μM of Sodium selenite, Treated: DMEM+100μM of Sodium selenite + Triphala. Incubation period 24 h. Values are mean±SD. *P<0.01 and #P <0.05 as compared to control. n=6.

Mentions: Changes in GSH and MDA levels were evaluated in lenses cultured in the presence of sodium selenite (100 μM) using different concentrations of Triphala. GSH levels in different groups are presented in Figure 3. GSH level in the normal group was estimated to be 1.08 ± 0.02 μmol/g of lens, whereas the GSH content of lenses in the control group was found to be 0.07 ± 0.007 μmol/g. Incorporation of Triphala into the culture medium significantly restored GSH levels. Values of 0.74 ± 0.02, 0.88 ± 0.03, and 0.84 ± 0.02 were obtained at concentrations of 400, 800, and 1200μg/ml, respectively. MDA levels in controls in the presence of sodium selenite produced the significant increase in lipid peroxidation of 41.33 ± 1.83 nmol/g of lens. However, in the presence of Triphala, lipid peroxidation was significantly reduced and MDA values were respectively found to be 20.93 ± 1.25, 18.82 ± 1.22, and 21.20 ± 1.13 nmol/g of lens at concentrations of 400, 800, and 1200 μg/ml [Figure 4].


Evaluation of anticataract potential of Triphala in selenite-induced cataract: In vitro and in vivo studies.

Gupta SK, Kalaiselvan V, Srivastava S, Agrawal SS, Saxena R - J Ayurveda Integr Med (2010)

Effect of Triphala on MDA levels in selenite-induced oxidative stress in vitro, Normal: DMEM, Control: DMEM+100μM of Sodium selenite, Treated: DMEM+100μM of Sodium selenite + Triphala. Incubation period 24 h. Values are mean±SD. *P<0.01 and #P <0.05 as compared to control. n=6.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3117320&req=5

Figure 0004: Effect of Triphala on MDA levels in selenite-induced oxidative stress in vitro, Normal: DMEM, Control: DMEM+100μM of Sodium selenite, Treated: DMEM+100μM of Sodium selenite + Triphala. Incubation period 24 h. Values are mean±SD. *P<0.01 and #P <0.05 as compared to control. n=6.
Mentions: Changes in GSH and MDA levels were evaluated in lenses cultured in the presence of sodium selenite (100 μM) using different concentrations of Triphala. GSH levels in different groups are presented in Figure 3. GSH level in the normal group was estimated to be 1.08 ± 0.02 μmol/g of lens, whereas the GSH content of lenses in the control group was found to be 0.07 ± 0.007 μmol/g. Incorporation of Triphala into the culture medium significantly restored GSH levels. Values of 0.74 ± 0.02, 0.88 ± 0.03, and 0.84 ± 0.02 were obtained at concentrations of 400, 800, and 1200μg/ml, respectively. MDA levels in controls in the presence of sodium selenite produced the significant increase in lipid peroxidation of 41.33 ± 1.83 nmol/g of lens. However, in the presence of Triphala, lipid peroxidation was significantly reduced and MDA values were respectively found to be 20.93 ± 1.25, 18.82 ± 1.22, and 21.20 ± 1.13 nmol/g of lens at concentrations of 400, 800, and 1200 μg/ml [Figure 4].

Bottom Line: TP significantly (P < 0.01) restored GSH and decreased malondialdehyde levels.A significant restoration in the activities of antioxidant enzymes such as superoxide dismutase (P < 0.05), catalase (P < 0.05), glutathione peroxidase (P < 0.05), and glutathione-s-transferase (P < 0.005) was observed in the TP-supplemented group compared to controls.This effect may be due to antioxidant activity.

View Article: PubMed Central - PubMed

Affiliation: Delhi Institute of Pharmaceutical Sciences and Research, New Delhi, India.

ABSTRACT
Triphala (TP) is composed of Emblica officinalis, Terminalia chebula, and Terminalia belerica. The present study was undertaken to evaluate its anticataract potential in vitro and in vivo in a selenite-induced experimental model of cataract. In vitro enucleated rat lenses were maintained in organ culture containing Dulbecco's Modified Eagles Medium alone or with the addition of 100μM selenite. These served as the normal and control groups, respectively. In the test group, the medium was supplemented with selenite and different concentrations of TP aqueous extract. The lenses were incubated for 24 h at 37°C. After incubation, the lenses were processed to estimate reduced glutathione (GSH), lipid peroxidation product, and antioxidant enzymes. In vivo selenite cataract was induced in 9-day-old rat pups by subcutaneous injection of sodium selenite (25 μmole/kg body weight). The test groups received 25, 50, and 75 mg/kg of TP intraperitoneally 4 h before the selenite challenge. At the end of the study period, the rats' eyes were examined by slit-lamp. TP significantly (P < 0.01) restored GSH and decreased malondialdehyde levels. A significant restoration in the activities of antioxidant enzymes such as superoxide dismutase (P < 0.05), catalase (P < 0.05), glutathione peroxidase (P < 0.05), and glutathione-s-transferase (P < 0.005) was observed in the TP-supplemented group compared to controls. In vivo TF 25mg/kg developed only 20% nuclear cataract as compared to 100% in control. TP prevents or retards experimental selenite-induced cataract. This effect may be due to antioxidant activity. Further studies are warranted to explore its role in human cataract.

No MeSH data available.


Related in: MedlinePlus