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Functional polymers in protein detection platforms: optical, electrochemical, electrical, mass-sensitive, and magnetic biosensors.

Hahm JI - Sensors (Basel) (2011)

Bottom Line: Materials utilized for such protein detection and measurement platforms should meet particular specifications which include ease-of-mass manufacture, biological stability, chemical functionality, cost effectiveness, and portability.Current challenges associated with the application of polymeric materials are examined in each protein detection category.The latest efforts on the development and evaluation of nanoscale polymeric systems for improved protein detection are also discussed from the standpoint of quantitative and qualitative measurements.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Georgetown University, 37th & O Sts. NW., Washington, DC 20057, USA. jh583@georgetown.edu

ABSTRACT
The rapidly growing field of proteomics and related applied sectors in the life sciences demands convenient methodologies for detecting and measuring the levels of specific proteins as well as for screening and analyzing for interacting protein systems. Materials utilized for such protein detection and measurement platforms should meet particular specifications which include ease-of-mass manufacture, biological stability, chemical functionality, cost effectiveness, and portability. Polymers can satisfy many of these requirements and are often considered as choice materials in various biological detection platforms. Therefore, tremendous research efforts have been made for developing new polymers both in macroscopic and nanoscopic length scales as well as applying existing polymeric materials for protein measurements. In this review article, both conventional and alternative techniques for protein detection are overviewed while focusing on the use of various polymeric materials in different protein sensing technologies. Among many available detection mechanisms, most common approaches such as optical, electrochemical, electrical, mass-sensitive, and magnetic methods are comprehensively discussed in this article. Desired properties of polymers exploited for each type of protein detection approach are summarized. Current challenges associated with the application of polymeric materials are examined in each protein detection category. Difficulties facing both quantitative and qualitative protein measurements are also identified. The latest efforts on the development and evaluation of nanoscale polymeric systems for improved protein detection are also discussed from the standpoint of quantitative and qualitative measurements. Finally, future research directions towards further advancements in the field are considered.

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More examples of functional polymers; (a) electrospun polydimethylsiloxane (PDMS)/polymethylmethacrylate (PMMA) nanofibers and (b) an integrated immunoassay device on cyclic olefin copolymer. Adapted with permission from [86] and [131].
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f3-sensors-11-03327: More examples of functional polymers; (a) electrospun polydimethylsiloxane (PDMS)/polymethylmethacrylate (PMMA) nanofibers and (b) an integrated immunoassay device on cyclic olefin copolymer. Adapted with permission from [86] and [131].

Mentions: Figure 3 shows (a) an electrospun nanofiber sensor consisting of PDMS/PMMA as described in the optical detection section and (b) an interdigitated array (IDA) for magnetic bead-based immunoassays fabricated into a lab-on-a-chip device on cyclic olefin copolymer.


Functional polymers in protein detection platforms: optical, electrochemical, electrical, mass-sensitive, and magnetic biosensors.

Hahm JI - Sensors (Basel) (2011)

More examples of functional polymers; (a) electrospun polydimethylsiloxane (PDMS)/polymethylmethacrylate (PMMA) nanofibers and (b) an integrated immunoassay device on cyclic olefin copolymer. Adapted with permission from [86] and [131].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3117287&req=5

f3-sensors-11-03327: More examples of functional polymers; (a) electrospun polydimethylsiloxane (PDMS)/polymethylmethacrylate (PMMA) nanofibers and (b) an integrated immunoassay device on cyclic olefin copolymer. Adapted with permission from [86] and [131].
Mentions: Figure 3 shows (a) an electrospun nanofiber sensor consisting of PDMS/PMMA as described in the optical detection section and (b) an interdigitated array (IDA) for magnetic bead-based immunoassays fabricated into a lab-on-a-chip device on cyclic olefin copolymer.

Bottom Line: Materials utilized for such protein detection and measurement platforms should meet particular specifications which include ease-of-mass manufacture, biological stability, chemical functionality, cost effectiveness, and portability.Current challenges associated with the application of polymeric materials are examined in each protein detection category.The latest efforts on the development and evaluation of nanoscale polymeric systems for improved protein detection are also discussed from the standpoint of quantitative and qualitative measurements.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Georgetown University, 37th & O Sts. NW., Washington, DC 20057, USA. jh583@georgetown.edu

ABSTRACT
The rapidly growing field of proteomics and related applied sectors in the life sciences demands convenient methodologies for detecting and measuring the levels of specific proteins as well as for screening and analyzing for interacting protein systems. Materials utilized for such protein detection and measurement platforms should meet particular specifications which include ease-of-mass manufacture, biological stability, chemical functionality, cost effectiveness, and portability. Polymers can satisfy many of these requirements and are often considered as choice materials in various biological detection platforms. Therefore, tremendous research efforts have been made for developing new polymers both in macroscopic and nanoscopic length scales as well as applying existing polymeric materials for protein measurements. In this review article, both conventional and alternative techniques for protein detection are overviewed while focusing on the use of various polymeric materials in different protein sensing technologies. Among many available detection mechanisms, most common approaches such as optical, electrochemical, electrical, mass-sensitive, and magnetic methods are comprehensively discussed in this article. Desired properties of polymers exploited for each type of protein detection approach are summarized. Current challenges associated with the application of polymeric materials are examined in each protein detection category. Difficulties facing both quantitative and qualitative protein measurements are also identified. The latest efforts on the development and evaluation of nanoscale polymeric systems for improved protein detection are also discussed from the standpoint of quantitative and qualitative measurements. Finally, future research directions towards further advancements in the field are considered.

Show MeSH