Limits...
Antibacterial and dermal toxicological profiles of ethyl acetate extract from Crassocephalum bauchiense (Hutch.) Milne-Redh (Asteraceae).

Mouokeu RS, Ngono RA, Lunga PK, Koanga MM, Tiabou AT, Njateng GS, Tamokou JD, Kuiate JR - BMC Complement Altern Med (2011)

Bottom Line: Crassocephalum bauchiense, a medicinal herb found in the West Region of Cameroon is used to treat gastrointestinal infections as well as liver disorders.Also, daily dermal application of the C. bauchiense extract gel formulation for 28 days did not show any negative effect, instead some biochemical parameters such as alanine aminotransferase (ALT and AST), low density lipoprotein (LDL), high density lipoprotein (HDL) and triglycerides were significantly (p < 0.05) affected positively.These results indicate that the C. bauchiense ethyl acetate extract can be used safely for the treatment of some bacterial infections.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Microbiology and Antimicrobial Substances, Faculty of Sciences, PO Box 67 Dschang - Cameroon.

ABSTRACT

Background: The emergence in recent years of numerous resistant strains of pathogenic bacteria to a range of formerly efficient antibiotics constitutes a serious threat to public health. Crassocephalum bauchiense, a medicinal herb found in the West Region of Cameroon is used to treat gastrointestinal infections as well as liver disorders. The ethyl acetate extract from the leaves of C. bauchiense was evaluated for its antibacterial activity as well as acute and sub-acute toxicities.

Methods: The plant extract was prepared by maceration in ethyl acetate. Its phytochemical screening was done by standard methods. The broth microdilution method was used to evaluate the in vitro antibacterial activity. The in vivo antibacterial activity of a gel formulation (0.05, 1 and 2% w/v) of this extract was evaluated using a Staphylococcus aureus-induced dermatitis in a murine model. Selected haematological and biochemical parameters were used to evaluate the dermal sub-acute toxicity of the extract in rats.

Results: Phytochemical screening of the C. bauchiense extract revealed the presence of alkaloids, phenols, tannins and sterols. In vitro antibacterial activities were observed against all the tested microorganisms (MIC = 0.04-6.25 mg/ml). Formulated extract-gel (2% w/v) and gentamycin (reference drug) eradicated the microbial infection after five days of treatment. A single dermal dose of this extract up to 32 g/kg body weight (bw) did not produce any visible sign of toxicity. Also, daily dermal application of the C. bauchiense extract gel formulation for 28 days did not show any negative effect, instead some biochemical parameters such as alanine aminotransferase (ALT and AST), low density lipoprotein (LDL), high density lipoprotein (HDL) and triglycerides were significantly (p < 0.05) affected positively.

Conclusion: These results indicate that the C. bauchiense ethyl acetate extract can be used safely for the treatment of some bacterial infections.

Show MeSH

Related in: MedlinePlus

Effect of the dose of gel-extract on weight gain as a function of time.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3116471&req=5

Figure 1: Effect of the dose of gel-extract on weight gain as a function of time.

Mentions: Animals infected with S. aureus showed visible inflammation 48 hours later, characterised by redness and swelling of the skin at the sites of inoculation. Treatment significantly (P < 0.05) reduced the bacterial concentration at the infection sites, with total suppression being noted with the 1 and 2% doses of C. bauchiense gel-extract as gentamycin in 5 days (Figure 1). Animals in all groups showed weight loss after infection (Figure 2). From day 1 of treatment, body weight increased gradually with time. On the other hand, the weight gain by the animals of the negative control was lower than that of all the other groups. However, the weight gain by the animals that received the extract at 1 and 2% were comparable (p > 0.05) to gentamycin group, and significantly higher (p < 0.05) than the 0.5% extract-treated group.


Antibacterial and dermal toxicological profiles of ethyl acetate extract from Crassocephalum bauchiense (Hutch.) Milne-Redh (Asteraceae).

Mouokeu RS, Ngono RA, Lunga PK, Koanga MM, Tiabou AT, Njateng GS, Tamokou JD, Kuiate JR - BMC Complement Altern Med (2011)

Effect of the dose of gel-extract on weight gain as a function of time.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3116471&req=5

Figure 1: Effect of the dose of gel-extract on weight gain as a function of time.
Mentions: Animals infected with S. aureus showed visible inflammation 48 hours later, characterised by redness and swelling of the skin at the sites of inoculation. Treatment significantly (P < 0.05) reduced the bacterial concentration at the infection sites, with total suppression being noted with the 1 and 2% doses of C. bauchiense gel-extract as gentamycin in 5 days (Figure 1). Animals in all groups showed weight loss after infection (Figure 2). From day 1 of treatment, body weight increased gradually with time. On the other hand, the weight gain by the animals of the negative control was lower than that of all the other groups. However, the weight gain by the animals that received the extract at 1 and 2% were comparable (p > 0.05) to gentamycin group, and significantly higher (p < 0.05) than the 0.5% extract-treated group.

Bottom Line: Crassocephalum bauchiense, a medicinal herb found in the West Region of Cameroon is used to treat gastrointestinal infections as well as liver disorders.Also, daily dermal application of the C. bauchiense extract gel formulation for 28 days did not show any negative effect, instead some biochemical parameters such as alanine aminotransferase (ALT and AST), low density lipoprotein (LDL), high density lipoprotein (HDL) and triglycerides were significantly (p < 0.05) affected positively.These results indicate that the C. bauchiense ethyl acetate extract can be used safely for the treatment of some bacterial infections.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Microbiology and Antimicrobial Substances, Faculty of Sciences, PO Box 67 Dschang - Cameroon.

ABSTRACT

Background: The emergence in recent years of numerous resistant strains of pathogenic bacteria to a range of formerly efficient antibiotics constitutes a serious threat to public health. Crassocephalum bauchiense, a medicinal herb found in the West Region of Cameroon is used to treat gastrointestinal infections as well as liver disorders. The ethyl acetate extract from the leaves of C. bauchiense was evaluated for its antibacterial activity as well as acute and sub-acute toxicities.

Methods: The plant extract was prepared by maceration in ethyl acetate. Its phytochemical screening was done by standard methods. The broth microdilution method was used to evaluate the in vitro antibacterial activity. The in vivo antibacterial activity of a gel formulation (0.05, 1 and 2% w/v) of this extract was evaluated using a Staphylococcus aureus-induced dermatitis in a murine model. Selected haematological and biochemical parameters were used to evaluate the dermal sub-acute toxicity of the extract in rats.

Results: Phytochemical screening of the C. bauchiense extract revealed the presence of alkaloids, phenols, tannins and sterols. In vitro antibacterial activities were observed against all the tested microorganisms (MIC = 0.04-6.25 mg/ml). Formulated extract-gel (2% w/v) and gentamycin (reference drug) eradicated the microbial infection after five days of treatment. A single dermal dose of this extract up to 32 g/kg body weight (bw) did not produce any visible sign of toxicity. Also, daily dermal application of the C. bauchiense extract gel formulation for 28 days did not show any negative effect, instead some biochemical parameters such as alanine aminotransferase (ALT and AST), low density lipoprotein (LDL), high density lipoprotein (HDL) and triglycerides were significantly (p < 0.05) affected positively.

Conclusion: These results indicate that the C. bauchiense ethyl acetate extract can be used safely for the treatment of some bacterial infections.

Show MeSH
Related in: MedlinePlus