Limits...
Design and characterization of protein-quercetin bioactive nanoparticles.

Fang R, Jing H, Chai Z, Zhao G, Stoll S, Ren F, Liu F, Leng X - J Nanobiotechnology (2011)

Bottom Line: After loading Q, the size was further reduced by 30%.The controlled releasing measurements indicate that these bioactive nanoparticles have long-term antioxidant protection effects on the activity of Q in both acidic and neutral conditions.BSA exhibits the most remarkable abilities of loading, controlled release, and antioxidant protection of active drugs, indicating that such type of bionanoparticles is very promising in the field of bionanotechnology.

View Article: PubMed Central - HTML - PubMed

Affiliation: CAU and ACC Joint Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

ABSTRACT

Background: The synthesis of bioactive nanoparticles with precise molecular level control is a major challenge in bionanotechnology. Understanding the nature of the interactions between the active components and transport biomaterials is thus essential for the rational formulation of bio-nanocarriers. The current study presents a single molecule of bovine serum albumin (BSA), lysozyme (Lys), or myoglobin (Mb) used to load hydrophobic drugs such as quercetin (Q) and other flavonoids.

Results: Induced by dimethyl sulfoxide (DMSO), BSA, Lys, and Mb formed spherical nanocarriers with sizes less than 70 nm. After loading Q, the size was further reduced by 30%. The adsorption of Q on protein is mainly hydrophobic, and is related to the synergy of Trp residues with the molecular environment of the proteins. Seven Q molecules could be entrapped by one Lys molecule, 9 by one Mb, and 11 by one BSA. The controlled releasing measurements indicate that these bioactive nanoparticles have long-term antioxidant protection effects on the activity of Q in both acidic and neutral conditions. The antioxidant activity evaluation indicates that the activity of Q is not hindered by the formation of protein nanoparticles. Other flavonoids, such as kaempferol and rutin, were also investigated.

Conclusions: BSA exhibits the most remarkable abilities of loading, controlled release, and antioxidant protection of active drugs, indicating that such type of bionanoparticles is very promising in the field of bionanotechnology.

Show MeSH
DPPH and ABTS scavenging activity of Q and embedded Q. The concentrations of Q was 1.50 × 10-5 mol/L. The concentration of the proteins (BSA, Lys, and Mb) was 1.5 × 10-6 mol/L. The (A) DPPH and (B) ABTS scavenging activities of the proteins were also subtracted from the embedded Q. Markers of different letters in the figure denote that the mean difference is significant at P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3116464&req=5

Figure 18: DPPH and ABTS scavenging activity of Q and embedded Q. The concentrations of Q was 1.50 × 10-5 mol/L. The concentration of the proteins (BSA, Lys, and Mb) was 1.5 × 10-6 mol/L. The (A) DPPH and (B) ABTS scavenging activities of the proteins were also subtracted from the embedded Q. Markers of different letters in the figure denote that the mean difference is significant at P < 0.05.

Mentions: DPPH and ABTS radical cation decolourization tests are spectrophotometric methods widely used to assess the antioxidant activity of various substances. Previous studies confirmed that Q has a high DPPH and ABTS antioxidant activity [54-56]. The present study compares the antioxidant activity of Q and embedded Q in BSA, Lys, and Mb nanocarriers. As shown in Figure 18A, the DPPH percent radical scavenging activity (% RSC) of Q was 82%, while the DPPH % RSC of all embedded Q did not change (P < 0.05) at all. Likewise, the ABTS % RSC of Q was 67.06%, while the ABTS % RSC of embedded Q in Lys and Mb nanocarriers did not change (P < 0.05); only the ABTS % RSC of embedded Q in the BSA nanocarriers decreased (P < 0.05) in comparison with free Q. This decrease, however, was so slight that it could be ignored (Figure 18B). Thus, antioxidant activity of Q was not interfered by protein nanoparticles.


Design and characterization of protein-quercetin bioactive nanoparticles.

Fang R, Jing H, Chai Z, Zhao G, Stoll S, Ren F, Liu F, Leng X - J Nanobiotechnology (2011)

DPPH and ABTS scavenging activity of Q and embedded Q. The concentrations of Q was 1.50 × 10-5 mol/L. The concentration of the proteins (BSA, Lys, and Mb) was 1.5 × 10-6 mol/L. The (A) DPPH and (B) ABTS scavenging activities of the proteins were also subtracted from the embedded Q. Markers of different letters in the figure denote that the mean difference is significant at P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3116464&req=5

Figure 18: DPPH and ABTS scavenging activity of Q and embedded Q. The concentrations of Q was 1.50 × 10-5 mol/L. The concentration of the proteins (BSA, Lys, and Mb) was 1.5 × 10-6 mol/L. The (A) DPPH and (B) ABTS scavenging activities of the proteins were also subtracted from the embedded Q. Markers of different letters in the figure denote that the mean difference is significant at P < 0.05.
Mentions: DPPH and ABTS radical cation decolourization tests are spectrophotometric methods widely used to assess the antioxidant activity of various substances. Previous studies confirmed that Q has a high DPPH and ABTS antioxidant activity [54-56]. The present study compares the antioxidant activity of Q and embedded Q in BSA, Lys, and Mb nanocarriers. As shown in Figure 18A, the DPPH percent radical scavenging activity (% RSC) of Q was 82%, while the DPPH % RSC of all embedded Q did not change (P < 0.05) at all. Likewise, the ABTS % RSC of Q was 67.06%, while the ABTS % RSC of embedded Q in Lys and Mb nanocarriers did not change (P < 0.05); only the ABTS % RSC of embedded Q in the BSA nanocarriers decreased (P < 0.05) in comparison with free Q. This decrease, however, was so slight that it could be ignored (Figure 18B). Thus, antioxidant activity of Q was not interfered by protein nanoparticles.

Bottom Line: After loading Q, the size was further reduced by 30%.The controlled releasing measurements indicate that these bioactive nanoparticles have long-term antioxidant protection effects on the activity of Q in both acidic and neutral conditions.BSA exhibits the most remarkable abilities of loading, controlled release, and antioxidant protection of active drugs, indicating that such type of bionanoparticles is very promising in the field of bionanotechnology.

View Article: PubMed Central - HTML - PubMed

Affiliation: CAU and ACC Joint Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

ABSTRACT

Background: The synthesis of bioactive nanoparticles with precise molecular level control is a major challenge in bionanotechnology. Understanding the nature of the interactions between the active components and transport biomaterials is thus essential for the rational formulation of bio-nanocarriers. The current study presents a single molecule of bovine serum albumin (BSA), lysozyme (Lys), or myoglobin (Mb) used to load hydrophobic drugs such as quercetin (Q) and other flavonoids.

Results: Induced by dimethyl sulfoxide (DMSO), BSA, Lys, and Mb formed spherical nanocarriers with sizes less than 70 nm. After loading Q, the size was further reduced by 30%. The adsorption of Q on protein is mainly hydrophobic, and is related to the synergy of Trp residues with the molecular environment of the proteins. Seven Q molecules could be entrapped by one Lys molecule, 9 by one Mb, and 11 by one BSA. The controlled releasing measurements indicate that these bioactive nanoparticles have long-term antioxidant protection effects on the activity of Q in both acidic and neutral conditions. The antioxidant activity evaluation indicates that the activity of Q is not hindered by the formation of protein nanoparticles. Other flavonoids, such as kaempferol and rutin, were also investigated.

Conclusions: BSA exhibits the most remarkable abilities of loading, controlled release, and antioxidant protection of active drugs, indicating that such type of bionanoparticles is very promising in the field of bionanotechnology.

Show MeSH