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Design and characterization of protein-quercetin bioactive nanoparticles.

Fang R, Jing H, Chai Z, Zhao G, Stoll S, Ren F, Liu F, Leng X - J Nanobiotechnology (2011)

Bottom Line: After loading Q, the size was further reduced by 30%.The controlled releasing measurements indicate that these bioactive nanoparticles have long-term antioxidant protection effects on the activity of Q in both acidic and neutral conditions.BSA exhibits the most remarkable abilities of loading, controlled release, and antioxidant protection of active drugs, indicating that such type of bionanoparticles is very promising in the field of bionanotechnology.

View Article: PubMed Central - HTML - PubMed

Affiliation: CAU and ACC Joint Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

ABSTRACT

Background: The synthesis of bioactive nanoparticles with precise molecular level control is a major challenge in bionanotechnology. Understanding the nature of the interactions between the active components and transport biomaterials is thus essential for the rational formulation of bio-nanocarriers. The current study presents a single molecule of bovine serum albumin (BSA), lysozyme (Lys), or myoglobin (Mb) used to load hydrophobic drugs such as quercetin (Q) and other flavonoids.

Results: Induced by dimethyl sulfoxide (DMSO), BSA, Lys, and Mb formed spherical nanocarriers with sizes less than 70 nm. After loading Q, the size was further reduced by 30%. The adsorption of Q on protein is mainly hydrophobic, and is related to the synergy of Trp residues with the molecular environment of the proteins. Seven Q molecules could be entrapped by one Lys molecule, 9 by one Mb, and 11 by one BSA. The controlled releasing measurements indicate that these bioactive nanoparticles have long-term antioxidant protection effects on the activity of Q in both acidic and neutral conditions. The antioxidant activity evaluation indicates that the activity of Q is not hindered by the formation of protein nanoparticles. Other flavonoids, such as kaempferol and rutin, were also investigated.

Conclusions: BSA exhibits the most remarkable abilities of loading, controlled release, and antioxidant protection of active drugs, indicating that such type of bionanoparticles is very promising in the field of bionanotechnology.

Show MeSH
UV-Vis spectra of free and bound Q to D-BSA, D-Lys, and D-Mb. The concentration of Q was 1.5 × 10-5 mol/L. The concentration of DMSO was maintained at 10%. The concentration of (A) BSA, (B) Lys, or (C) Mb was 1.5 × 10-5 mol/L. The solid line represents free Q, and the dashed line represents bound Q.
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Figure 15: UV-Vis spectra of free and bound Q to D-BSA, D-Lys, and D-Mb. The concentration of Q was 1.5 × 10-5 mol/L. The concentration of DMSO was maintained at 10%. The concentration of (A) BSA, (B) Lys, or (C) Mb was 1.5 × 10-5 mol/L. The solid line represents free Q, and the dashed line represents bound Q.

Mentions: Figure 15 compares the UV-Vis absorption spectra of Q, D-BSA-Q (A), D-BSA-Q (B), and D-Mb-Q (C). The pure Q showed its characteristic band at 367 nm, which is associated with the cinnamoyl group [16]. Normally, the formation of H-bonds between the chromophoric group of Q and auxochromic group can result in an obvious red shift [48-50]; this was found when Q was mixed with BSA (A). No shift of this band was found when Q was mixed with Lys (B) or Mb (C), indicating no H-bonds formed between Q and the two proteins. Thus, the quantity of Q bound to Lys and Mb was probably less than that bound to BSA.


Design and characterization of protein-quercetin bioactive nanoparticles.

Fang R, Jing H, Chai Z, Zhao G, Stoll S, Ren F, Liu F, Leng X - J Nanobiotechnology (2011)

UV-Vis spectra of free and bound Q to D-BSA, D-Lys, and D-Mb. The concentration of Q was 1.5 × 10-5 mol/L. The concentration of DMSO was maintained at 10%. The concentration of (A) BSA, (B) Lys, or (C) Mb was 1.5 × 10-5 mol/L. The solid line represents free Q, and the dashed line represents bound Q.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3116464&req=5

Figure 15: UV-Vis spectra of free and bound Q to D-BSA, D-Lys, and D-Mb. The concentration of Q was 1.5 × 10-5 mol/L. The concentration of DMSO was maintained at 10%. The concentration of (A) BSA, (B) Lys, or (C) Mb was 1.5 × 10-5 mol/L. The solid line represents free Q, and the dashed line represents bound Q.
Mentions: Figure 15 compares the UV-Vis absorption spectra of Q, D-BSA-Q (A), D-BSA-Q (B), and D-Mb-Q (C). The pure Q showed its characteristic band at 367 nm, which is associated with the cinnamoyl group [16]. Normally, the formation of H-bonds between the chromophoric group of Q and auxochromic group can result in an obvious red shift [48-50]; this was found when Q was mixed with BSA (A). No shift of this band was found when Q was mixed with Lys (B) or Mb (C), indicating no H-bonds formed between Q and the two proteins. Thus, the quantity of Q bound to Lys and Mb was probably less than that bound to BSA.

Bottom Line: After loading Q, the size was further reduced by 30%.The controlled releasing measurements indicate that these bioactive nanoparticles have long-term antioxidant protection effects on the activity of Q in both acidic and neutral conditions.BSA exhibits the most remarkable abilities of loading, controlled release, and antioxidant protection of active drugs, indicating that such type of bionanoparticles is very promising in the field of bionanotechnology.

View Article: PubMed Central - HTML - PubMed

Affiliation: CAU and ACC Joint Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

ABSTRACT

Background: The synthesis of bioactive nanoparticles with precise molecular level control is a major challenge in bionanotechnology. Understanding the nature of the interactions between the active components and transport biomaterials is thus essential for the rational formulation of bio-nanocarriers. The current study presents a single molecule of bovine serum albumin (BSA), lysozyme (Lys), or myoglobin (Mb) used to load hydrophobic drugs such as quercetin (Q) and other flavonoids.

Results: Induced by dimethyl sulfoxide (DMSO), BSA, Lys, and Mb formed spherical nanocarriers with sizes less than 70 nm. After loading Q, the size was further reduced by 30%. The adsorption of Q on protein is mainly hydrophobic, and is related to the synergy of Trp residues with the molecular environment of the proteins. Seven Q molecules could be entrapped by one Lys molecule, 9 by one Mb, and 11 by one BSA. The controlled releasing measurements indicate that these bioactive nanoparticles have long-term antioxidant protection effects on the activity of Q in both acidic and neutral conditions. The antioxidant activity evaluation indicates that the activity of Q is not hindered by the formation of protein nanoparticles. Other flavonoids, such as kaempferol and rutin, were also investigated.

Conclusions: BSA exhibits the most remarkable abilities of loading, controlled release, and antioxidant protection of active drugs, indicating that such type of bionanoparticles is very promising in the field of bionanotechnology.

Show MeSH