Limits...
Design and characterization of protein-quercetin bioactive nanoparticles.

Fang R, Jing H, Chai Z, Zhao G, Stoll S, Ren F, Liu F, Leng X - J Nanobiotechnology (2011)

Bottom Line: After loading Q, the size was further reduced by 30%.The controlled releasing measurements indicate that these bioactive nanoparticles have long-term antioxidant protection effects on the activity of Q in both acidic and neutral conditions.BSA exhibits the most remarkable abilities of loading, controlled release, and antioxidant protection of active drugs, indicating that such type of bionanoparticles is very promising in the field of bionanotechnology.

View Article: PubMed Central - HTML - PubMed

Affiliation: CAU and ACC Joint Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

ABSTRACT

Background: The synthesis of bioactive nanoparticles with precise molecular level control is a major challenge in bionanotechnology. Understanding the nature of the interactions between the active components and transport biomaterials is thus essential for the rational formulation of bio-nanocarriers. The current study presents a single molecule of bovine serum albumin (BSA), lysozyme (Lys), or myoglobin (Mb) used to load hydrophobic drugs such as quercetin (Q) and other flavonoids.

Results: Induced by dimethyl sulfoxide (DMSO), BSA, Lys, and Mb formed spherical nanocarriers with sizes less than 70 nm. After loading Q, the size was further reduced by 30%. The adsorption of Q on protein is mainly hydrophobic, and is related to the synergy of Trp residues with the molecular environment of the proteins. Seven Q molecules could be entrapped by one Lys molecule, 9 by one Mb, and 11 by one BSA. The controlled releasing measurements indicate that these bioactive nanoparticles have long-term antioxidant protection effects on the activity of Q in both acidic and neutral conditions. The antioxidant activity evaluation indicates that the activity of Q is not hindered by the formation of protein nanoparticles. Other flavonoids, such as kaempferol and rutin, were also investigated.

Conclusions: BSA exhibits the most remarkable abilities of loading, controlled release, and antioxidant protection of active drugs, indicating that such type of bionanoparticles is very promising in the field of bionanotechnology.

Show MeSH
Raman spectrum of Mb system vs. the concentration of Q. The concentrations of Mb and DMSO were maintained at 1.5 × 10-5 mol/L and 10%, respectively. (a) 0 mol/L Q; (b) 3.0 × 10-5 mol/L Q; (c) 9.0 × 10-5 mol/L Q; (d) 1.5 × 10-4 mol/L Q.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3116464&req=5

Figure 12: Raman spectrum of Mb system vs. the concentration of Q. The concentrations of Mb and DMSO were maintained at 1.5 × 10-5 mol/L and 10%, respectively. (a) 0 mol/L Q; (b) 3.0 × 10-5 mol/L Q; (c) 9.0 × 10-5 mol/L Q; (d) 1.5 × 10-4 mol/L Q.

Mentions: Mb consists of eight helical regions and a non-covalent bound heme prosthetic group, which is buried in a relatively hydrophobic pocket interior of the protein. With laser excitation, the Raman bands of the porphyrin skeleton, appearing between 1650 and 1100 cm-1, become very intense and disturb the signals of the other bands (Figure 12). This phenomenon brings difficulty in the analysis in this region [21,37]. In addition, the approach of two Trp residues to the heme results in a partial energy transfer of the chromophoric group in Trp [37], and causes the Raman bands arising from Trp, such as those at 1611, 1319, and 600 cm-1, to become very weak (Figure 12).


Design and characterization of protein-quercetin bioactive nanoparticles.

Fang R, Jing H, Chai Z, Zhao G, Stoll S, Ren F, Liu F, Leng X - J Nanobiotechnology (2011)

Raman spectrum of Mb system vs. the concentration of Q. The concentrations of Mb and DMSO were maintained at 1.5 × 10-5 mol/L and 10%, respectively. (a) 0 mol/L Q; (b) 3.0 × 10-5 mol/L Q; (c) 9.0 × 10-5 mol/L Q; (d) 1.5 × 10-4 mol/L Q.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3116464&req=5

Figure 12: Raman spectrum of Mb system vs. the concentration of Q. The concentrations of Mb and DMSO were maintained at 1.5 × 10-5 mol/L and 10%, respectively. (a) 0 mol/L Q; (b) 3.0 × 10-5 mol/L Q; (c) 9.0 × 10-5 mol/L Q; (d) 1.5 × 10-4 mol/L Q.
Mentions: Mb consists of eight helical regions and a non-covalent bound heme prosthetic group, which is buried in a relatively hydrophobic pocket interior of the protein. With laser excitation, the Raman bands of the porphyrin skeleton, appearing between 1650 and 1100 cm-1, become very intense and disturb the signals of the other bands (Figure 12). This phenomenon brings difficulty in the analysis in this region [21,37]. In addition, the approach of two Trp residues to the heme results in a partial energy transfer of the chromophoric group in Trp [37], and causes the Raman bands arising from Trp, such as those at 1611, 1319, and 600 cm-1, to become very weak (Figure 12).

Bottom Line: After loading Q, the size was further reduced by 30%.The controlled releasing measurements indicate that these bioactive nanoparticles have long-term antioxidant protection effects on the activity of Q in both acidic and neutral conditions.BSA exhibits the most remarkable abilities of loading, controlled release, and antioxidant protection of active drugs, indicating that such type of bionanoparticles is very promising in the field of bionanotechnology.

View Article: PubMed Central - HTML - PubMed

Affiliation: CAU and ACC Joint Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

ABSTRACT

Background: The synthesis of bioactive nanoparticles with precise molecular level control is a major challenge in bionanotechnology. Understanding the nature of the interactions between the active components and transport biomaterials is thus essential for the rational formulation of bio-nanocarriers. The current study presents a single molecule of bovine serum albumin (BSA), lysozyme (Lys), or myoglobin (Mb) used to load hydrophobic drugs such as quercetin (Q) and other flavonoids.

Results: Induced by dimethyl sulfoxide (DMSO), BSA, Lys, and Mb formed spherical nanocarriers with sizes less than 70 nm. After loading Q, the size was further reduced by 30%. The adsorption of Q on protein is mainly hydrophobic, and is related to the synergy of Trp residues with the molecular environment of the proteins. Seven Q molecules could be entrapped by one Lys molecule, 9 by one Mb, and 11 by one BSA. The controlled releasing measurements indicate that these bioactive nanoparticles have long-term antioxidant protection effects on the activity of Q in both acidic and neutral conditions. The antioxidant activity evaluation indicates that the activity of Q is not hindered by the formation of protein nanoparticles. Other flavonoids, such as kaempferol and rutin, were also investigated.

Conclusions: BSA exhibits the most remarkable abilities of loading, controlled release, and antioxidant protection of active drugs, indicating that such type of bionanoparticles is very promising in the field of bionanotechnology.

Show MeSH