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Evaluation of Composition and Antimicrobial Activity of Supercritical Fluid Extract of Leaves of Vitex negundo.

Nagarsekar KS, Nagarsenker MS, Kulkarni SR - Indian J Pharm Sci (2010)

Bottom Line: Supercritical fluid extract of leaves of Vitex negundo was tested for its antimicrobial potential and was compared with that of ethanol extract, ether extract and hydrodistilled oil of leaves.The chemical constituents of extracts were studied by chromatographic techniques.Supercritical fluid extract exhibited good antibacterial potential.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy and Phytochemistry, Bombay College of Pharmacy, Kalina, Mumbai-400 098, India.

ABSTRACT
Supercritical fluid extract of leaves of Vitex negundo was tested for its antimicrobial potential and was compared with that of ethanol extract, ether extract and hydrodistilled oil of leaves. The chemical constituents of extracts were studied by chromatographic techniques. Extracts were evaluated for antimicrobial potential against bacterial strains like Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and yeast Candida albicans. Extracts showed prominent antibacterial activity against Bacillus subtilis and Staphylococcus aureus. Supercritical fluid extract exhibited good antibacterial potential.

No MeSH data available.


Related in: MedlinePlus

TLC pattern of various extracts of leaves of Vitex negundo (a) fl avonoids in ethanol extract of leaves of Vitex negundo, track 1 and 2 indicate TLC pattern exposed to visible light and track 3 and 4 at 366nm after derivatisation with NP/PEG reagent (b) TLC pattern of supercritical fl uid extract of leaves of Vitex negundo observed at 366nm (track 3 and 4) and in visible light after derivatisation with Anisaldehyde sulphuric acid reagent (track 1 and 2). (c) TLC pattern of hydrodistilled oil of leaves of Vitex negundo observed at 254nm (track 3 and 4) and in visible light after derivatisation with Anisaldehyde sulphuric acid reagent (track 1 and 2).
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Figure 1: TLC pattern of various extracts of leaves of Vitex negundo (a) fl avonoids in ethanol extract of leaves of Vitex negundo, track 1 and 2 indicate TLC pattern exposed to visible light and track 3 and 4 at 366nm after derivatisation with NP/PEG reagent (b) TLC pattern of supercritical fl uid extract of leaves of Vitex negundo observed at 366nm (track 3 and 4) and in visible light after derivatisation with Anisaldehyde sulphuric acid reagent (track 1 and 2). (c) TLC pattern of hydrodistilled oil of leaves of Vitex negundo observed at 254nm (track 3 and 4) and in visible light after derivatisation with Anisaldehyde sulphuric acid reagent (track 1 and 2).

Mentions: Percent yields of various extracts of leaves of Vitex negundo Linn. are given in Table 1. TLC studies showed that the ethanol extract contained flavonoids as the major constituents along with a few terpenoids (fig. 1a). Essential oils (terpenoids) were found to be the major constituents of supercritical fluid extract (fig. 1b) and steam distilled oil (fig. 1c). Petroleum ether extract contained terpenoids and chlorophyll derivatives.


Evaluation of Composition and Antimicrobial Activity of Supercritical Fluid Extract of Leaves of Vitex negundo.

Nagarsekar KS, Nagarsenker MS, Kulkarni SR - Indian J Pharm Sci (2010)

TLC pattern of various extracts of leaves of Vitex negundo (a) fl avonoids in ethanol extract of leaves of Vitex negundo, track 1 and 2 indicate TLC pattern exposed to visible light and track 3 and 4 at 366nm after derivatisation with NP/PEG reagent (b) TLC pattern of supercritical fl uid extract of leaves of Vitex negundo observed at 366nm (track 3 and 4) and in visible light after derivatisation with Anisaldehyde sulphuric acid reagent (track 1 and 2). (c) TLC pattern of hydrodistilled oil of leaves of Vitex negundo observed at 254nm (track 3 and 4) and in visible light after derivatisation with Anisaldehyde sulphuric acid reagent (track 1 and 2).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3116313&req=5

Figure 1: TLC pattern of various extracts of leaves of Vitex negundo (a) fl avonoids in ethanol extract of leaves of Vitex negundo, track 1 and 2 indicate TLC pattern exposed to visible light and track 3 and 4 at 366nm after derivatisation with NP/PEG reagent (b) TLC pattern of supercritical fl uid extract of leaves of Vitex negundo observed at 366nm (track 3 and 4) and in visible light after derivatisation with Anisaldehyde sulphuric acid reagent (track 1 and 2). (c) TLC pattern of hydrodistilled oil of leaves of Vitex negundo observed at 254nm (track 3 and 4) and in visible light after derivatisation with Anisaldehyde sulphuric acid reagent (track 1 and 2).
Mentions: Percent yields of various extracts of leaves of Vitex negundo Linn. are given in Table 1. TLC studies showed that the ethanol extract contained flavonoids as the major constituents along with a few terpenoids (fig. 1a). Essential oils (terpenoids) were found to be the major constituents of supercritical fluid extract (fig. 1b) and steam distilled oil (fig. 1c). Petroleum ether extract contained terpenoids and chlorophyll derivatives.

Bottom Line: Supercritical fluid extract of leaves of Vitex negundo was tested for its antimicrobial potential and was compared with that of ethanol extract, ether extract and hydrodistilled oil of leaves.The chemical constituents of extracts were studied by chromatographic techniques.Supercritical fluid extract exhibited good antibacterial potential.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy and Phytochemistry, Bombay College of Pharmacy, Kalina, Mumbai-400 098, India.

ABSTRACT
Supercritical fluid extract of leaves of Vitex negundo was tested for its antimicrobial potential and was compared with that of ethanol extract, ether extract and hydrodistilled oil of leaves. The chemical constituents of extracts were studied by chromatographic techniques. Extracts were evaluated for antimicrobial potential against bacterial strains like Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and yeast Candida albicans. Extracts showed prominent antibacterial activity against Bacillus subtilis and Staphylococcus aureus. Supercritical fluid extract exhibited good antibacterial potential.

No MeSH data available.


Related in: MedlinePlus