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Development and Validation of HPTLC Method for the Estimation of Almotriptan Malate in Tablet Dosage Form.

Suneetha A, Syamasundar B - Indian J Pharm Sci (2010)

Bottom Line: The method was validated for linearity, accuracy, precision and robustness.The calibration plot was linear over the range of 100-700 ng/band for almotriptan malate.The method was successfully applied to the analysis of drug in a pharmaceutical dosage form.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur-522 002, India.

ABSTRACT
A new, simple, precise and accurate high performance thin layer chromatographic method has been proposed for the determination of almotriptan malate in a tablet dosage form. The drug was separated on aluminum plates precoated with silica gel 60 GF(254) with butanol:acetic acid:water (3:1:1) was used as mobilephase. Quantitative analysis was performed by densitometric scanning at 300 nm. The method was validated for linearity, accuracy, precision and robustness. The calibration plot was linear over the range of 100-700 ng/band for almotriptan malate. The method was successfully applied to the analysis of drug in a pharmaceutical dosage form.

No MeSH data available.


Overlain spectra of almotriptan malate from 200 to 400 nmrecorded in situ on the plate --■--100 ng/band; --×--200 ng/band;--◆--300 ng/band;--●-- 400 ng/band; --Δ--500 ng/band; --*--600 ng/band;--+-- 700 ng/band
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Figure 1: Overlain spectra of almotriptan malate from 200 to 400 nmrecorded in situ on the plate --■--100 ng/band; --×--200 ng/band;--◆--300 ng/band;--●-- 400 ng/band; --Δ--500 ng/band; --*--600 ng/band;--+-- 700 ng/band

Mentions: After chromatographic development, bands were scanned over the range 200-400 nm (spectrum scan speed 20 nm/s) so that the drug could be estimated at 300 nm fig. 1, which is ascertained by taking the spectrum at different concentrations between 100-700 ng with 100 ng increment. Further it is also observed that spectra are similar in their behavior.


Development and Validation of HPTLC Method for the Estimation of Almotriptan Malate in Tablet Dosage Form.

Suneetha A, Syamasundar B - Indian J Pharm Sci (2010)

Overlain spectra of almotriptan malate from 200 to 400 nmrecorded in situ on the plate --■--100 ng/band; --×--200 ng/band;--◆--300 ng/band;--●-- 400 ng/band; --Δ--500 ng/band; --*--600 ng/band;--+-- 700 ng/band
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3116310&req=5

Figure 1: Overlain spectra of almotriptan malate from 200 to 400 nmrecorded in situ on the plate --■--100 ng/band; --×--200 ng/band;--◆--300 ng/band;--●-- 400 ng/band; --Δ--500 ng/band; --*--600 ng/band;--+-- 700 ng/band
Mentions: After chromatographic development, bands were scanned over the range 200-400 nm (spectrum scan speed 20 nm/s) so that the drug could be estimated at 300 nm fig. 1, which is ascertained by taking the spectrum at different concentrations between 100-700 ng with 100 ng increment. Further it is also observed that spectra are similar in their behavior.

Bottom Line: The method was validated for linearity, accuracy, precision and robustness.The calibration plot was linear over the range of 100-700 ng/band for almotriptan malate.The method was successfully applied to the analysis of drug in a pharmaceutical dosage form.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur-522 002, India.

ABSTRACT
A new, simple, precise and accurate high performance thin layer chromatographic method has been proposed for the determination of almotriptan malate in a tablet dosage form. The drug was separated on aluminum plates precoated with silica gel 60 GF(254) with butanol:acetic acid:water (3:1:1) was used as mobilephase. Quantitative analysis was performed by densitometric scanning at 300 nm. The method was validated for linearity, accuracy, precision and robustness. The calibration plot was linear over the range of 100-700 ng/band for almotriptan malate. The method was successfully applied to the analysis of drug in a pharmaceutical dosage form.

No MeSH data available.