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In vitro antioxidant activities of sulfated derivatives of polysaccharides extracted from Auricularia auricular.

Zhang H, Wang ZY, Yang L, Yang X, Wang X, Zhang Z - Int J Mol Sci (2011)

Bottom Line: In this research, two types of sulfated polysaccharide derivatives were successfully synthesized.Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity.In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

View Article: PubMed Central - PubMed

Affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China; E-Mails: zhhua@hit.edu.cn (H.Z.); ly6617@hit.edu.cn (L.Y.); yangxin@hit.edu.cn (X.Y.).

ABSTRACT
In this research, two types of sulfated polysaccharide derivatives were successfully synthesized. Their antioxidant activities were investigated by employing various established in vitro systems. In addition, the degree of sulfation was evaluated using ion-chromatography and IR spectra. The results verify that, when employing scavenging superoxide radical tests, both the sulfation of acid Auricularia auricular polysaccharides (SAAAP) and the sulfation of neutral Auricularia auricular polysaccharides (SNAAP) derivatives possessed considerable antioxidant activity and had a more powerful antioxidant competence than that of the native non-sulfated polysaccharides (AAAP and NAAP). On the other hand, AAAP and NAAP exhibited stronger activity on scavenging both the hydroxyl radical and lipid peroxidation. Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity. In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

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Lipid peroxidation by AAAP, SAAAP, NAAP, and SNAAP.
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f6-ijms-12-03288: Lipid peroxidation by AAAP, SAAAP, NAAP, and SNAAP.

Mentions: Cellular damage is closely related with lipid peroxidation and causes aging, carcinogenesis and many other diseases [42]. Liposomes are used extensively as biological models for in vitro lipid peroxidation studies [27,43]. Therefore, in this research the liposomal system was employed to evaluate the inhibitory activities of the A.auricular polysaccharide extracts toward lipid peroxidation. The results are noteworthy when compared to the findings of other studies concerning medicinal plants [44]. The study of lipid peroxidation is now increasing due to interest in finding natural herbal plants that can prevent not only lipid peroxidation in foods, but also oxidative damage to living cells. As illustrated in Figure 6, the lipid peroxidation inhibitory effect of polysaccharides (including AAAP and NAAP) increased in two patterns with increasing concentrations, while the scavenging activity of AAAP and NAAP decreased when the concentration range increased from 1.0 to 5.0 mg/mL. The sulfated Auricularia auricular polysaccharide derivatives, when tested over 0.2–5.0 mg/mL, did not show scavenging activity against lipid peroxidation. The EC50 of these polysaccharides was unreadable. Previous studies have reported that the antioxidant mechanism was the same as the cleaning hydroxyl radical when generated [5]. Therefore, the tendency of inhibitory activity against lipid peroxidation of Auricularia auricular polysaccharides (including SAAAP, SNAAP, AAAP, and NAAP) was consistent with the scavenging ability against the hydroxyl radicals.


In vitro antioxidant activities of sulfated derivatives of polysaccharides extracted from Auricularia auricular.

Zhang H, Wang ZY, Yang L, Yang X, Wang X, Zhang Z - Int J Mol Sci (2011)

Lipid peroxidation by AAAP, SAAAP, NAAP, and SNAAP.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116191&req=5

f6-ijms-12-03288: Lipid peroxidation by AAAP, SAAAP, NAAP, and SNAAP.
Mentions: Cellular damage is closely related with lipid peroxidation and causes aging, carcinogenesis and many other diseases [42]. Liposomes are used extensively as biological models for in vitro lipid peroxidation studies [27,43]. Therefore, in this research the liposomal system was employed to evaluate the inhibitory activities of the A.auricular polysaccharide extracts toward lipid peroxidation. The results are noteworthy when compared to the findings of other studies concerning medicinal plants [44]. The study of lipid peroxidation is now increasing due to interest in finding natural herbal plants that can prevent not only lipid peroxidation in foods, but also oxidative damage to living cells. As illustrated in Figure 6, the lipid peroxidation inhibitory effect of polysaccharides (including AAAP and NAAP) increased in two patterns with increasing concentrations, while the scavenging activity of AAAP and NAAP decreased when the concentration range increased from 1.0 to 5.0 mg/mL. The sulfated Auricularia auricular polysaccharide derivatives, when tested over 0.2–5.0 mg/mL, did not show scavenging activity against lipid peroxidation. The EC50 of these polysaccharides was unreadable. Previous studies have reported that the antioxidant mechanism was the same as the cleaning hydroxyl radical when generated [5]. Therefore, the tendency of inhibitory activity against lipid peroxidation of Auricularia auricular polysaccharides (including SAAAP, SNAAP, AAAP, and NAAP) was consistent with the scavenging ability against the hydroxyl radicals.

Bottom Line: In this research, two types of sulfated polysaccharide derivatives were successfully synthesized.Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity.In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

View Article: PubMed Central - PubMed

Affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China; E-Mails: zhhua@hit.edu.cn (H.Z.); ly6617@hit.edu.cn (L.Y.); yangxin@hit.edu.cn (X.Y.).

ABSTRACT
In this research, two types of sulfated polysaccharide derivatives were successfully synthesized. Their antioxidant activities were investigated by employing various established in vitro systems. In addition, the degree of sulfation was evaluated using ion-chromatography and IR spectra. The results verify that, when employing scavenging superoxide radical tests, both the sulfation of acid Auricularia auricular polysaccharides (SAAAP) and the sulfation of neutral Auricularia auricular polysaccharides (SNAAP) derivatives possessed considerable antioxidant activity and had a more powerful antioxidant competence than that of the native non-sulfated polysaccharides (AAAP and NAAP). On the other hand, AAAP and NAAP exhibited stronger activity on scavenging both the hydroxyl radical and lipid peroxidation. Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity. In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

Show MeSH
Related in: MedlinePlus