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In vitro antioxidant activities of sulfated derivatives of polysaccharides extracted from Auricularia auricular.

Zhang H, Wang ZY, Yang L, Yang X, Wang X, Zhang Z - Int J Mol Sci (2011)

Bottom Line: In this research, two types of sulfated polysaccharide derivatives were successfully synthesized.Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity.In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

View Article: PubMed Central - PubMed

Affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China; E-Mails: zhhua@hit.edu.cn (H.Z.); ly6617@hit.edu.cn (L.Y.); yangxin@hit.edu.cn (X.Y.).

ABSTRACT
In this research, two types of sulfated polysaccharide derivatives were successfully synthesized. Their antioxidant activities were investigated by employing various established in vitro systems. In addition, the degree of sulfation was evaluated using ion-chromatography and IR spectra. The results verify that, when employing scavenging superoxide radical tests, both the sulfation of acid Auricularia auricular polysaccharides (SAAAP) and the sulfation of neutral Auricularia auricular polysaccharides (SNAAP) derivatives possessed considerable antioxidant activity and had a more powerful antioxidant competence than that of the native non-sulfated polysaccharides (AAAP and NAAP). On the other hand, AAAP and NAAP exhibited stronger activity on scavenging both the hydroxyl radical and lipid peroxidation. Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity. In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

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Related in: MedlinePlus

ABTS radical scavenging activity by AAAP, SAAAP, NAAP, and SNAAP.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3116191&req=5

f5-ijms-12-03288: ABTS radical scavenging activity by AAAP, SAAAP, NAAP, and SNAAP.

Mentions: The reactions with ABTS+• radicals involve a single-electron transfer process. Bleaching of a preformed solution of the blue-green radical cation ABTS+•, which has an absorption at 734 nm, has been extensively used by past researchers to evaluate the antioxidant capacity of complex mixtures and individual compounds [5]. As illustrated in Figure 5, the ABTS radical-scavenging activities of polysaccharides (including SAAAP, AAAP, SNAAP and NAAP) increased in four patterns with increased concentrations. A fast increase for native non-sulfated polysaccharide (NAAP and AAAP) and a slow increase for sulfated polysaccharides ranged from 0.1 to 6.67 mg/mL. The EC50 of these polysaccharides was also unreadable. The results also indicate that native non-sulfated Auricularia auricular polysaccharides and their sulfated derivatives are effective as natural antioxidants.


In vitro antioxidant activities of sulfated derivatives of polysaccharides extracted from Auricularia auricular.

Zhang H, Wang ZY, Yang L, Yang X, Wang X, Zhang Z - Int J Mol Sci (2011)

ABTS radical scavenging activity by AAAP, SAAAP, NAAP, and SNAAP.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116191&req=5

f5-ijms-12-03288: ABTS radical scavenging activity by AAAP, SAAAP, NAAP, and SNAAP.
Mentions: The reactions with ABTS+• radicals involve a single-electron transfer process. Bleaching of a preformed solution of the blue-green radical cation ABTS+•, which has an absorption at 734 nm, has been extensively used by past researchers to evaluate the antioxidant capacity of complex mixtures and individual compounds [5]. As illustrated in Figure 5, the ABTS radical-scavenging activities of polysaccharides (including SAAAP, AAAP, SNAAP and NAAP) increased in four patterns with increased concentrations. A fast increase for native non-sulfated polysaccharide (NAAP and AAAP) and a slow increase for sulfated polysaccharides ranged from 0.1 to 6.67 mg/mL. The EC50 of these polysaccharides was also unreadable. The results also indicate that native non-sulfated Auricularia auricular polysaccharides and their sulfated derivatives are effective as natural antioxidants.

Bottom Line: In this research, two types of sulfated polysaccharide derivatives were successfully synthesized.Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity.In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

View Article: PubMed Central - PubMed

Affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China; E-Mails: zhhua@hit.edu.cn (H.Z.); ly6617@hit.edu.cn (L.Y.); yangxin@hit.edu.cn (X.Y.).

ABSTRACT
In this research, two types of sulfated polysaccharide derivatives were successfully synthesized. Their antioxidant activities were investigated by employing various established in vitro systems. In addition, the degree of sulfation was evaluated using ion-chromatography and IR spectra. The results verify that, when employing scavenging superoxide radical tests, both the sulfation of acid Auricularia auricular polysaccharides (SAAAP) and the sulfation of neutral Auricularia auricular polysaccharides (SNAAP) derivatives possessed considerable antioxidant activity and had a more powerful antioxidant competence than that of the native non-sulfated polysaccharides (AAAP and NAAP). On the other hand, AAAP and NAAP exhibited stronger activity on scavenging both the hydroxyl radical and lipid peroxidation. Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity. In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

Show MeSH
Related in: MedlinePlus