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In vitro antioxidant activities of sulfated derivatives of polysaccharides extracted from Auricularia auricular.

Zhang H, Wang ZY, Yang L, Yang X, Wang X, Zhang Z - Int J Mol Sci (2011)

Bottom Line: In this research, two types of sulfated polysaccharide derivatives were successfully synthesized.Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity.In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

View Article: PubMed Central - PubMed

Affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China; E-Mails: zhhua@hit.edu.cn (H.Z.); ly6617@hit.edu.cn (L.Y.); yangxin@hit.edu.cn (X.Y.).

ABSTRACT
In this research, two types of sulfated polysaccharide derivatives were successfully synthesized. Their antioxidant activities were investigated by employing various established in vitro systems. In addition, the degree of sulfation was evaluated using ion-chromatography and IR spectra. The results verify that, when employing scavenging superoxide radical tests, both the sulfation of acid Auricularia auricular polysaccharides (SAAAP) and the sulfation of neutral Auricularia auricular polysaccharides (SNAAP) derivatives possessed considerable antioxidant activity and had a more powerful antioxidant competence than that of the native non-sulfated polysaccharides (AAAP and NAAP). On the other hand, AAAP and NAAP exhibited stronger activity on scavenging both the hydroxyl radical and lipid peroxidation. Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity. In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

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Scavenging effect on hydroxyl radicals by AAAP, SAAAP, NAAP, and SNAAP.
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f4-ijms-12-03288: Scavenging effect on hydroxyl radicals by AAAP, SAAAP, NAAP, and SNAAP.

Mentions: The hydroxyl radicals, generated by the Fenton reaction, were scavenged by AAAP, NAAP and its sulfated derivatives (Figure 4). Not all the sulfated derivatives exhibited stronger scavenging ability against hydroxyl radicals than the native non-sulfated polysaccharide did (AAAP and NAAP). For example, the activities of AAAP and NAAP increased the range from 0.2 to 1.0 mg/mL, while the activities (y of AAAP and NAAP) decreased when the concentration increased from 1.0 to 5.0 mg/mL range. The sulfated derivatives ranging from 0.2 to 5.0 mg/mL did not exhibit any scavenging ability against the hydroxyl radicals. The EC50 of these polysaccharides could not be read.


In vitro antioxidant activities of sulfated derivatives of polysaccharides extracted from Auricularia auricular.

Zhang H, Wang ZY, Yang L, Yang X, Wang X, Zhang Z - Int J Mol Sci (2011)

Scavenging effect on hydroxyl radicals by AAAP, SAAAP, NAAP, and SNAAP.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116191&req=5

f4-ijms-12-03288: Scavenging effect on hydroxyl radicals by AAAP, SAAAP, NAAP, and SNAAP.
Mentions: The hydroxyl radicals, generated by the Fenton reaction, were scavenged by AAAP, NAAP and its sulfated derivatives (Figure 4). Not all the sulfated derivatives exhibited stronger scavenging ability against hydroxyl radicals than the native non-sulfated polysaccharide did (AAAP and NAAP). For example, the activities of AAAP and NAAP increased the range from 0.2 to 1.0 mg/mL, while the activities (y of AAAP and NAAP) decreased when the concentration increased from 1.0 to 5.0 mg/mL range. The sulfated derivatives ranging from 0.2 to 5.0 mg/mL did not exhibit any scavenging ability against the hydroxyl radicals. The EC50 of these polysaccharides could not be read.

Bottom Line: In this research, two types of sulfated polysaccharide derivatives were successfully synthesized.Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity.In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

View Article: PubMed Central - PubMed

Affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China; E-Mails: zhhua@hit.edu.cn (H.Z.); ly6617@hit.edu.cn (L.Y.); yangxin@hit.edu.cn (X.Y.).

ABSTRACT
In this research, two types of sulfated polysaccharide derivatives were successfully synthesized. Their antioxidant activities were investigated by employing various established in vitro systems. In addition, the degree of sulfation was evaluated using ion-chromatography and IR spectra. The results verify that, when employing scavenging superoxide radical tests, both the sulfation of acid Auricularia auricular polysaccharides (SAAAP) and the sulfation of neutral Auricularia auricular polysaccharides (SNAAP) derivatives possessed considerable antioxidant activity and had a more powerful antioxidant competence than that of the native non-sulfated polysaccharides (AAAP and NAAP). On the other hand, AAAP and NAAP exhibited stronger activity on scavenging both the hydroxyl radical and lipid peroxidation. Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity. In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

Show MeSH
Related in: MedlinePlus