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In vitro antioxidant activities of sulfated derivatives of polysaccharides extracted from Auricularia auricular.

Zhang H, Wang ZY, Yang L, Yang X, Wang X, Zhang Z - Int J Mol Sci (2011)

Bottom Line: In this research, two types of sulfated polysaccharide derivatives were successfully synthesized.Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity.In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

View Article: PubMed Central - PubMed

Affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China; E-Mails: zhhua@hit.edu.cn (H.Z.); ly6617@hit.edu.cn (L.Y.); yangxin@hit.edu.cn (X.Y.).

ABSTRACT
In this research, two types of sulfated polysaccharide derivatives were successfully synthesized. Their antioxidant activities were investigated by employing various established in vitro systems. In addition, the degree of sulfation was evaluated using ion-chromatography and IR spectra. The results verify that, when employing scavenging superoxide radical tests, both the sulfation of acid Auricularia auricular polysaccharides (SAAAP) and the sulfation of neutral Auricularia auricular polysaccharides (SNAAP) derivatives possessed considerable antioxidant activity and had a more powerful antioxidant competence than that of the native non-sulfated polysaccharides (AAAP and NAAP). On the other hand, AAAP and NAAP exhibited stronger activity on scavenging both the hydroxyl radical and lipid peroxidation. Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity. In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

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Fourier Transform Infrared (FTIR) spectra of AAAP, SAAAP, NAAP, and SNAAP.
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f2-ijms-12-03288: Fourier Transform Infrared (FTIR) spectra of AAAP, SAAAP, NAAP, and SNAAP.

Mentions: An FT-IR spectroscopy analysis was used to investigate the vibrations of molecules and polar bonds between the different atoms. It is possible to analyze structures of polysaccharides such as monosaccharide types, glucosidic bonds, and functional groups using an FT-IR spectroscopy [29]. The SAAAP and SNAAP in the frequency range of 4000–400 cm−1 were then pulverized and blended with a KBr powder, which was then pressed into pellets for the FT-IR measurement (Figure 2). In all spectra, the band in the region of 3411.1 and 3410.5 cm−1 of SAAAP and SNAAP corresponds to the hydroxyl stretching vibration of the polysaccharide and at 2923.3 and 2921.3 cm−1 corresponds to a weak C–H stretching vibration. This amalgamation indicates that the SAAAP and SNAAP were all polysaccharides. By comparison with AAAP and NAAP, two characteristic absorption bands appeared in the FT-IR spectra of the sulfated derivatives (Figure 2): One at 1258 cm−1 describing an asymmetrical S=O stretching vibration and the other at 810 cm−1 representing a symmetrical C–O–S vibration associated with a C–O–SO3 group, which signifies that they were successfully sulfated [31].


In vitro antioxidant activities of sulfated derivatives of polysaccharides extracted from Auricularia auricular.

Zhang H, Wang ZY, Yang L, Yang X, Wang X, Zhang Z - Int J Mol Sci (2011)

Fourier Transform Infrared (FTIR) spectra of AAAP, SAAAP, NAAP, and SNAAP.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116191&req=5

f2-ijms-12-03288: Fourier Transform Infrared (FTIR) spectra of AAAP, SAAAP, NAAP, and SNAAP.
Mentions: An FT-IR spectroscopy analysis was used to investigate the vibrations of molecules and polar bonds between the different atoms. It is possible to analyze structures of polysaccharides such as monosaccharide types, glucosidic bonds, and functional groups using an FT-IR spectroscopy [29]. The SAAAP and SNAAP in the frequency range of 4000–400 cm−1 were then pulverized and blended with a KBr powder, which was then pressed into pellets for the FT-IR measurement (Figure 2). In all spectra, the band in the region of 3411.1 and 3410.5 cm−1 of SAAAP and SNAAP corresponds to the hydroxyl stretching vibration of the polysaccharide and at 2923.3 and 2921.3 cm−1 corresponds to a weak C–H stretching vibration. This amalgamation indicates that the SAAAP and SNAAP were all polysaccharides. By comparison with AAAP and NAAP, two characteristic absorption bands appeared in the FT-IR spectra of the sulfated derivatives (Figure 2): One at 1258 cm−1 describing an asymmetrical S=O stretching vibration and the other at 810 cm−1 representing a symmetrical C–O–S vibration associated with a C–O–SO3 group, which signifies that they were successfully sulfated [31].

Bottom Line: In this research, two types of sulfated polysaccharide derivatives were successfully synthesized.Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity.In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

View Article: PubMed Central - PubMed

Affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China; E-Mails: zhhua@hit.edu.cn (H.Z.); ly6617@hit.edu.cn (L.Y.); yangxin@hit.edu.cn (X.Y.).

ABSTRACT
In this research, two types of sulfated polysaccharide derivatives were successfully synthesized. Their antioxidant activities were investigated by employing various established in vitro systems. In addition, the degree of sulfation was evaluated using ion-chromatography and IR spectra. The results verify that, when employing scavenging superoxide radical tests, both the sulfation of acid Auricularia auricular polysaccharides (SAAAP) and the sulfation of neutral Auricularia auricular polysaccharides (SNAAP) derivatives possessed considerable antioxidant activity and had a more powerful antioxidant competence than that of the native non-sulfated polysaccharides (AAAP and NAAP). On the other hand, AAAP and NAAP exhibited stronger activity on scavenging both the hydroxyl radical and lipid peroxidation. Available data obtained with in vitro measurements indicates that the sulfated groups of AAAP and NAAP played an important role on antioxidant activity. In sum, the research demonstrates that the antioxidant activity of sulfated polysaccharide derivatives in vitro has a potential significance for seeking new natural antioxidant protective agents.

Show MeSH
Related in: MedlinePlus