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Enhancing effect of glycerol on the tensile properties of Bombyx mori cocoon sericin films.

Zhang H, Deng L, Yang M, Min S, Yang L, Zhu L - Int J Mol Sci (2011)

Bottom Line: An environmental physical method described herein was developed to improve the tensile properties of Bombyx mori cocoon sericin films, by using the plasticizer of glycerol, which has a nontoxic effect compared with other chemical crosslinkers.Scanning Electron Microscopy (SEM) observation revealed that glycerol was homogeneously blended with sericin molecules when its content was 10 wt%, while a small amount of redundant glycerol emerged on the surface of sericin films when its content was increased to 20 wt% or higher.Our results suggest that the introduction of glycerol is a novel nontoxic strategy which can improve the mechanical features of sericin-based materials and subsequently promote the feasibility of its application in tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Institute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China; E-Mails: hpzhang@zju.edu.cn (H.Z.); denglianxia2008@163.com (L.D.); yangm@zju.edu.cn (M.Y.); minsj@zju.edu.cn (S.M.); yanglei164@163.com (L.Y.).

ABSTRACT
An environmental physical method described herein was developed to improve the tensile properties of Bombyx mori cocoon sericin films, by using the plasticizer of glycerol, which has a nontoxic effect compared with other chemical crosslinkers. The changes in the tensile characteristics and the structure of glycerolated (0-40 wt% of glycerol) sericin films were investigated. Sericin films, both in dry and wet states, showed enhanced tensile properties, which might be regulated by the addition of different concentrations of glycerol. The introduction of glycerol results in the higher amorphous structure in sericin films as evidenced by analysis of attenuated total reflection Fourier transform infrared (ATR-FTIR) spectra, thermogravimetry (TGA) and differential scanning calorimetry (DSC) curves. Scanning Electron Microscopy (SEM) observation revealed that glycerol was homogeneously blended with sericin molecules when its content was 10 wt%, while a small amount of redundant glycerol emerged on the surface of sericin films when its content was increased to 20 wt% or higher. Our results suggest that the introduction of glycerol is a novel nontoxic strategy which can improve the mechanical features of sericin-based materials and subsequently promote the feasibility of its application in tissue engineering.

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SEM images of various sericin films: (A) sericin film; (B) sericin film with 10 wt% glycerol; (C) sericin film with 20 wt% glycerol; (D) sericin film with 30 wt% glycerol; (E) sericin film with 40 wt% glycerol.
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f6-ijms-12-03170: SEM images of various sericin films: (A) sericin film; (B) sericin film with 10 wt% glycerol; (C) sericin film with 20 wt% glycerol; (D) sericin film with 30 wt% glycerol; (E) sericin film with 40 wt% glycerol.

Mentions: The morphology change of sericin films after the addition of glycerol is shown in Figure 6. Both sericin films without glycerol and with 10 wt% glycerol showed smooth surface (Figure 6A,B), which implied that glycerol is homogeneously distributed in the films with 10 wt% glycerol. However, when the glycerol content was increased more than 20 wt%, many protuberances were observed on the surface of these films (Figure 6C–E) due to the aggregation of the redundant glycerol molecules. In the case of sericin films with 20 wt% glycerol, the length of protuberances was 15–25 μm and the width was 3–7 μm. When the content of glycerol in the film was increased to 30 wt%, the length and width increased to 17–44 μm and 11–30μm, respectively. The protuberances increased to 27–82 μm in the length and 16–39 μm in the width when the content of glycerol is 40 wt%. The number of protuberances in sericin films with 20 wt%, 30 wt% and 40 wt% glycerol were about 233, 97 and 66, respectively, in the area of 590 μm × 350 μm. These results indicated that the size of the protuberances increased with the content of glycerol while their numbers decreased.


Enhancing effect of glycerol on the tensile properties of Bombyx mori cocoon sericin films.

Zhang H, Deng L, Yang M, Min S, Yang L, Zhu L - Int J Mol Sci (2011)

SEM images of various sericin films: (A) sericin film; (B) sericin film with 10 wt% glycerol; (C) sericin film with 20 wt% glycerol; (D) sericin film with 30 wt% glycerol; (E) sericin film with 40 wt% glycerol.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116183&req=5

f6-ijms-12-03170: SEM images of various sericin films: (A) sericin film; (B) sericin film with 10 wt% glycerol; (C) sericin film with 20 wt% glycerol; (D) sericin film with 30 wt% glycerol; (E) sericin film with 40 wt% glycerol.
Mentions: The morphology change of sericin films after the addition of glycerol is shown in Figure 6. Both sericin films without glycerol and with 10 wt% glycerol showed smooth surface (Figure 6A,B), which implied that glycerol is homogeneously distributed in the films with 10 wt% glycerol. However, when the glycerol content was increased more than 20 wt%, many protuberances were observed on the surface of these films (Figure 6C–E) due to the aggregation of the redundant glycerol molecules. In the case of sericin films with 20 wt% glycerol, the length of protuberances was 15–25 μm and the width was 3–7 μm. When the content of glycerol in the film was increased to 30 wt%, the length and width increased to 17–44 μm and 11–30μm, respectively. The protuberances increased to 27–82 μm in the length and 16–39 μm in the width when the content of glycerol is 40 wt%. The number of protuberances in sericin films with 20 wt%, 30 wt% and 40 wt% glycerol were about 233, 97 and 66, respectively, in the area of 590 μm × 350 μm. These results indicated that the size of the protuberances increased with the content of glycerol while their numbers decreased.

Bottom Line: An environmental physical method described herein was developed to improve the tensile properties of Bombyx mori cocoon sericin films, by using the plasticizer of glycerol, which has a nontoxic effect compared with other chemical crosslinkers.Scanning Electron Microscopy (SEM) observation revealed that glycerol was homogeneously blended with sericin molecules when its content was 10 wt%, while a small amount of redundant glycerol emerged on the surface of sericin films when its content was increased to 20 wt% or higher.Our results suggest that the introduction of glycerol is a novel nontoxic strategy which can improve the mechanical features of sericin-based materials and subsequently promote the feasibility of its application in tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Institute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China; E-Mails: hpzhang@zju.edu.cn (H.Z.); denglianxia2008@163.com (L.D.); yangm@zju.edu.cn (M.Y.); minsj@zju.edu.cn (S.M.); yanglei164@163.com (L.Y.).

ABSTRACT
An environmental physical method described herein was developed to improve the tensile properties of Bombyx mori cocoon sericin films, by using the plasticizer of glycerol, which has a nontoxic effect compared with other chemical crosslinkers. The changes in the tensile characteristics and the structure of glycerolated (0-40 wt% of glycerol) sericin films were investigated. Sericin films, both in dry and wet states, showed enhanced tensile properties, which might be regulated by the addition of different concentrations of glycerol. The introduction of glycerol results in the higher amorphous structure in sericin films as evidenced by analysis of attenuated total reflection Fourier transform infrared (ATR-FTIR) spectra, thermogravimetry (TGA) and differential scanning calorimetry (DSC) curves. Scanning Electron Microscopy (SEM) observation revealed that glycerol was homogeneously blended with sericin molecules when its content was 10 wt%, while a small amount of redundant glycerol emerged on the surface of sericin films when its content was increased to 20 wt% or higher. Our results suggest that the introduction of glycerol is a novel nontoxic strategy which can improve the mechanical features of sericin-based materials and subsequently promote the feasibility of its application in tissue engineering.

Show MeSH