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Genetic diversity and phylogeny of antagonistic bacteria against Phytophthora nicotianae isolated from tobacco rhizosphere.

Jin F, Ding Y, Ding W, Reddy MS, Fernando WG, Du B - Int J Mol Sci (2011)

Bottom Line: A total of 25 16S-RFLP patterns were identified representing over 33 species from 17 different genera.Our results also found a significant amount of bacterial diversity among the antagonistic bacteria compared to other published reports.For the first time; Delftia tsuruhatensis, Stenotrophomonas maltophilia, Advenella incenata, Bacillus altitudinis, Kocuria palustris, Bacillus licheniformis, Agrobacterium tumefaciens and Myroides odoratimimus are reported to display antagonistic activity towards Phytophthora nicotianae.

View Article: PubMed Central - PubMed

Affiliation: Shandong Key Laboratory of Agricultural Microbiology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China; E-Mails: jfl315@163.com (F.J.); dingyq6885@163.com (Y.D.).

ABSTRACT
The genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by BOXAIR-PCR, 16S-RFLP, 16S rRNA sequence homology and phylogenetic analysis methods. These studies revealed that 4.01% of the 6652 tested had some inhibitory activity against Phytophthora nicotianae. BOXAIR-PCR analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. A total of 25 16S-RFLP patterns were identified representing over 33 species from 17 different genera. Our results also found a significant amount of bacterial diversity among the antagonistic bacteria compared to other published reports. For the first time; Delftia tsuruhatensis, Stenotrophomonas maltophilia, Advenella incenata, Bacillus altitudinis, Kocuria palustris, Bacillus licheniformis, Agrobacterium tumefaciens and Myroides odoratimimus are reported to display antagonistic activity towards Phytophthora nicotianae. Furthermore, the majority (75%) of the isolates assayed for antagonistic activity were Gram-positives compared to only 25% that were Gram-negative bacteria.

Show MeSH
Screening of antagonistic bacteria used plate confrontation method in PDA (Potato Dextrose Agar) plates. In the middle of the agar plates are the Phytophthora nicotianae, around the Phytophthora nicotianae are parts of the antagonistic bacteria.
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f1-ijms-12-03055: Screening of antagonistic bacteria used plate confrontation method in PDA (Potato Dextrose Agar) plates. In the middle of the agar plates are the Phytophthora nicotianae, around the Phytophthora nicotianae are parts of the antagonistic bacteria.

Mentions: The bacterial detection and purification experiments were repeated three times and led to the eventual identification of a total of 267 bacteria strains demonstrating antagonistic activity against Phytophthora nicotianae. These isolates had varying zones of inhibition ranging from large to smaller inhibition zones (1–10 mm), with 31 bacteria strongly active, with inhibition zones larger than 10 mm. (Figure 1). Results showed that 4.01% of the culturable bacterial isolates were active against Phytophthora nicotianae, and 0.47% of the culturable bacterial isolates had a stable and efficient capability to control Phytophthora nicotianae and could be used for the prevention of Black shank disease during tobacco production.


Genetic diversity and phylogeny of antagonistic bacteria against Phytophthora nicotianae isolated from tobacco rhizosphere.

Jin F, Ding Y, Ding W, Reddy MS, Fernando WG, Du B - Int J Mol Sci (2011)

Screening of antagonistic bacteria used plate confrontation method in PDA (Potato Dextrose Agar) plates. In the middle of the agar plates are the Phytophthora nicotianae, around the Phytophthora nicotianae are parts of the antagonistic bacteria.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116175&req=5

f1-ijms-12-03055: Screening of antagonistic bacteria used plate confrontation method in PDA (Potato Dextrose Agar) plates. In the middle of the agar plates are the Phytophthora nicotianae, around the Phytophthora nicotianae are parts of the antagonistic bacteria.
Mentions: The bacterial detection and purification experiments were repeated three times and led to the eventual identification of a total of 267 bacteria strains demonstrating antagonistic activity against Phytophthora nicotianae. These isolates had varying zones of inhibition ranging from large to smaller inhibition zones (1–10 mm), with 31 bacteria strongly active, with inhibition zones larger than 10 mm. (Figure 1). Results showed that 4.01% of the culturable bacterial isolates were active against Phytophthora nicotianae, and 0.47% of the culturable bacterial isolates had a stable and efficient capability to control Phytophthora nicotianae and could be used for the prevention of Black shank disease during tobacco production.

Bottom Line: A total of 25 16S-RFLP patterns were identified representing over 33 species from 17 different genera.Our results also found a significant amount of bacterial diversity among the antagonistic bacteria compared to other published reports.For the first time; Delftia tsuruhatensis, Stenotrophomonas maltophilia, Advenella incenata, Bacillus altitudinis, Kocuria palustris, Bacillus licheniformis, Agrobacterium tumefaciens and Myroides odoratimimus are reported to display antagonistic activity towards Phytophthora nicotianae.

View Article: PubMed Central - PubMed

Affiliation: Shandong Key Laboratory of Agricultural Microbiology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China; E-Mails: jfl315@163.com (F.J.); dingyq6885@163.com (Y.D.).

ABSTRACT
The genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by BOXAIR-PCR, 16S-RFLP, 16S rRNA sequence homology and phylogenetic analysis methods. These studies revealed that 4.01% of the 6652 tested had some inhibitory activity against Phytophthora nicotianae. BOXAIR-PCR analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. A total of 25 16S-RFLP patterns were identified representing over 33 species from 17 different genera. Our results also found a significant amount of bacterial diversity among the antagonistic bacteria compared to other published reports. For the first time; Delftia tsuruhatensis, Stenotrophomonas maltophilia, Advenella incenata, Bacillus altitudinis, Kocuria palustris, Bacillus licheniformis, Agrobacterium tumefaciens and Myroides odoratimimus are reported to display antagonistic activity towards Phytophthora nicotianae. Furthermore, the majority (75%) of the isolates assayed for antagonistic activity were Gram-positives compared to only 25% that were Gram-negative bacteria.

Show MeSH