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Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology.

Zou TB, Wang M, Gan RY, Ling WH - Int J Mol Sci (2011)

Bottom Line: Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities.This study investigated the ultrasound-assisted extraction (UAE) of anthocyanins from mulberry by using response surface methodology (RSM).The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China; E-Mails: zoutb@163.com (T.-B.Z.); wangmin1605@tom.com (M.W.); ganry_zsu@yahoo.cn (R.-Y.G.).

ABSTRACT
Mulberry is one of the most widely used traditional Chinese medicines. Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities. This study investigated the ultrasound-assisted extraction (UAE) of anthocyanins from mulberry by using response surface methodology (RSM). The extraction conditions associated with anthocyanin yield, including extraction solvent, liquid-to-solid rate, temperature and extraction time, are discussed. The optimal conditions obtained by RSM for UAE from mulberry include 63.8% methanol contains 1% (v/v) trifluoroacetic acid (TFA), 43.2 °C temperature, 23.8 (v/w) liquid-to-solid ratio, and 40 min time for the maximum yield (64.70 ± 0.45 mg/g). The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.

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Related in: MedlinePlus

Response surface graphs for the effects of methanol concentration, temperature and liquid-to-solid ratio on anthocyanin yield of mulberry extract: (A) Methanol concentration (X1) and temperature (X2); (B) Methanol concentration (X1) and liquid-to-solid ratio (X3); (C) Temperature (X2) and liquid-to-solid ratio (X3).
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Related In: Results  -  Collection

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f3-ijms-12-03006: Response surface graphs for the effects of methanol concentration, temperature and liquid-to-solid ratio on anthocyanin yield of mulberry extract: (A) Methanol concentration (X1) and temperature (X2); (B) Methanol concentration (X1) and liquid-to-solid ratio (X3); (C) Temperature (X2) and liquid-to-solid ratio (X3).

Mentions: Three-dimensional response surface plots are presented in Figure 3. An increase of methanol concentration (X1) and liquid-to-solid ratio (X3) result in an increase of anthocyanin yield to a maximum at a certain level, while an increase of temperature (X2) results in an initial increase of anthocyanin yield that then decreases when the temperature continues to rise.


Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology.

Zou TB, Wang M, Gan RY, Ling WH - Int J Mol Sci (2011)

Response surface graphs for the effects of methanol concentration, temperature and liquid-to-solid ratio on anthocyanin yield of mulberry extract: (A) Methanol concentration (X1) and temperature (X2); (B) Methanol concentration (X1) and liquid-to-solid ratio (X3); (C) Temperature (X2) and liquid-to-solid ratio (X3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116171&req=5

f3-ijms-12-03006: Response surface graphs for the effects of methanol concentration, temperature and liquid-to-solid ratio on anthocyanin yield of mulberry extract: (A) Methanol concentration (X1) and temperature (X2); (B) Methanol concentration (X1) and liquid-to-solid ratio (X3); (C) Temperature (X2) and liquid-to-solid ratio (X3).
Mentions: Three-dimensional response surface plots are presented in Figure 3. An increase of methanol concentration (X1) and liquid-to-solid ratio (X3) result in an increase of anthocyanin yield to a maximum at a certain level, while an increase of temperature (X2) results in an initial increase of anthocyanin yield that then decreases when the temperature continues to rise.

Bottom Line: Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities.This study investigated the ultrasound-assisted extraction (UAE) of anthocyanins from mulberry by using response surface methodology (RSM).The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China; E-Mails: zoutb@163.com (T.-B.Z.); wangmin1605@tom.com (M.W.); ganry_zsu@yahoo.cn (R.-Y.G.).

ABSTRACT
Mulberry is one of the most widely used traditional Chinese medicines. Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities. This study investigated the ultrasound-assisted extraction (UAE) of anthocyanins from mulberry by using response surface methodology (RSM). The extraction conditions associated with anthocyanin yield, including extraction solvent, liquid-to-solid rate, temperature and extraction time, are discussed. The optimal conditions obtained by RSM for UAE from mulberry include 63.8% methanol contains 1% (v/v) trifluoroacetic acid (TFA), 43.2 °C temperature, 23.8 (v/w) liquid-to-solid ratio, and 40 min time for the maximum yield (64.70 ± 0.45 mg/g). The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.

Show MeSH
Related in: MedlinePlus