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Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology.

Zou TB, Wang M, Gan RY, Ling WH - Int J Mol Sci (2011)

Bottom Line: Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities.The extraction conditions associated with anthocyanin yield, including extraction solvent, liquid-to-solid rate, temperature and extraction time, are discussed.The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China; E-Mails: zoutb@163.com (T.-B.Z.); wangmin1605@tom.com (M.W.); ganry_zsu@yahoo.cn (R.-Y.G.).

ABSTRACT
Mulberry is one of the most widely used traditional Chinese medicines. Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities. This study investigated the ultrasound-assisted extraction (UAE) of anthocyanins from mulberry by using response surface methodology (RSM). The extraction conditions associated with anthocyanin yield, including extraction solvent, liquid-to-solid rate, temperature and extraction time, are discussed. The optimal conditions obtained by RSM for UAE from mulberry include 63.8% methanol contains 1% (v/v) trifluoroacetic acid (TFA), 43.2 °C temperature, 23.8 (v/w) liquid-to-solid ratio, and 40 min time for the maximum yield (64.70 ± 0.45 mg/g). The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.

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Related in: MedlinePlus

Response surface graphs for the effects of methanol concentration, temperature and liquid-to-solid ratio on anthocyanin yield of mulberry extract: (A) Methanol concentration (X1) and temperature (X2); (B) Methanol concentration (X1) and liquid-to-solid ratio (X3); (C) Temperature (X2) and liquid-to-solid ratio (X3).
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Related In: Results  -  Collection

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f3-ijms-12-03006: Response surface graphs for the effects of methanol concentration, temperature and liquid-to-solid ratio on anthocyanin yield of mulberry extract: (A) Methanol concentration (X1) and temperature (X2); (B) Methanol concentration (X1) and liquid-to-solid ratio (X3); (C) Temperature (X2) and liquid-to-solid ratio (X3).

Mentions: Three-dimensional response surface plots are presented in Figure 3. An increase of methanol concentration (X1) and liquid-to-solid ratio (X3) result in an increase of anthocyanin yield to a maximum at a certain level, while an increase of temperature (X2) results in an initial increase of anthocyanin yield that then decreases when the temperature continues to rise.


Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology.

Zou TB, Wang M, Gan RY, Ling WH - Int J Mol Sci (2011)

Response surface graphs for the effects of methanol concentration, temperature and liquid-to-solid ratio on anthocyanin yield of mulberry extract: (A) Methanol concentration (X1) and temperature (X2); (B) Methanol concentration (X1) and liquid-to-solid ratio (X3); (C) Temperature (X2) and liquid-to-solid ratio (X3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116171&req=5

f3-ijms-12-03006: Response surface graphs for the effects of methanol concentration, temperature and liquid-to-solid ratio on anthocyanin yield of mulberry extract: (A) Methanol concentration (X1) and temperature (X2); (B) Methanol concentration (X1) and liquid-to-solid ratio (X3); (C) Temperature (X2) and liquid-to-solid ratio (X3).
Mentions: Three-dimensional response surface plots are presented in Figure 3. An increase of methanol concentration (X1) and liquid-to-solid ratio (X3) result in an increase of anthocyanin yield to a maximum at a certain level, while an increase of temperature (X2) results in an initial increase of anthocyanin yield that then decreases when the temperature continues to rise.

Bottom Line: Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities.The extraction conditions associated with anthocyanin yield, including extraction solvent, liquid-to-solid rate, temperature and extraction time, are discussed.The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China; E-Mails: zoutb@163.com (T.-B.Z.); wangmin1605@tom.com (M.W.); ganry_zsu@yahoo.cn (R.-Y.G.).

ABSTRACT
Mulberry is one of the most widely used traditional Chinese medicines. Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities. This study investigated the ultrasound-assisted extraction (UAE) of anthocyanins from mulberry by using response surface methodology (RSM). The extraction conditions associated with anthocyanin yield, including extraction solvent, liquid-to-solid rate, temperature and extraction time, are discussed. The optimal conditions obtained by RSM for UAE from mulberry include 63.8% methanol contains 1% (v/v) trifluoroacetic acid (TFA), 43.2 °C temperature, 23.8 (v/w) liquid-to-solid ratio, and 40 min time for the maximum yield (64.70 ± 0.45 mg/g). The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.

Show MeSH
Related in: MedlinePlus