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Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology.

Zou TB, Wang M, Gan RY, Ling WH - Int J Mol Sci (2011)

Bottom Line: Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities.The extraction conditions associated with anthocyanin yield, including extraction solvent, liquid-to-solid rate, temperature and extraction time, are discussed.The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China; E-Mails: zoutb@163.com (T.-B.Z.); wangmin1605@tom.com (M.W.); ganry_zsu@yahoo.cn (R.-Y.G.).

ABSTRACT
Mulberry is one of the most widely used traditional Chinese medicines. Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities. This study investigated the ultrasound-assisted extraction (UAE) of anthocyanins from mulberry by using response surface methodology (RSM). The extraction conditions associated with anthocyanin yield, including extraction solvent, liquid-to-solid rate, temperature and extraction time, are discussed. The optimal conditions obtained by RSM for UAE from mulberry include 63.8% methanol contains 1% (v/v) trifluoroacetic acid (TFA), 43.2 °C temperature, 23.8 (v/w) liquid-to-solid ratio, and 40 min time for the maximum yield (64.70 ± 0.45 mg/g). The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.

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Related in: MedlinePlus

The chromatograms of cyanidin-3-glucoside (A), cyanidin-3-rutinoside (B) standard substance and mulberry extract (C) at λ = 520 nm.
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f1-ijms-12-03006: The chromatograms of cyanidin-3-glucoside (A), cyanidin-3-rutinoside (B) standard substance and mulberry extract (C) at λ = 520 nm.

Mentions: The chromatograms of standard substance and the sample are shown in Figure 1. The authentic cyanidin-3-glucoside and cyanidin-3-rutinoside had a retention time of 9.4 min (Figure 1A) and 10.3 min (Figure 1B), respectively. The chromatogram of ultrasonically extracted sample is shown in Figure 1C. Anthocyanin yield was quantified by using calibration curves and expressed as mg/g (other anthocyanins were expressed as the amount of cyanidin-3-glucoside).


Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology.

Zou TB, Wang M, Gan RY, Ling WH - Int J Mol Sci (2011)

The chromatograms of cyanidin-3-glucoside (A), cyanidin-3-rutinoside (B) standard substance and mulberry extract (C) at λ = 520 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116171&req=5

f1-ijms-12-03006: The chromatograms of cyanidin-3-glucoside (A), cyanidin-3-rutinoside (B) standard substance and mulberry extract (C) at λ = 520 nm.
Mentions: The chromatograms of standard substance and the sample are shown in Figure 1. The authentic cyanidin-3-glucoside and cyanidin-3-rutinoside had a retention time of 9.4 min (Figure 1A) and 10.3 min (Figure 1B), respectively. The chromatogram of ultrasonically extracted sample is shown in Figure 1C. Anthocyanin yield was quantified by using calibration curves and expressed as mg/g (other anthocyanins were expressed as the amount of cyanidin-3-glucoside).

Bottom Line: Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities.The extraction conditions associated with anthocyanin yield, including extraction solvent, liquid-to-solid rate, temperature and extraction time, are discussed.The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China; E-Mails: zoutb@163.com (T.-B.Z.); wangmin1605@tom.com (M.W.); ganry_zsu@yahoo.cn (R.-Y.G.).

ABSTRACT
Mulberry is one of the most widely used traditional Chinese medicines. Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities. This study investigated the ultrasound-assisted extraction (UAE) of anthocyanins from mulberry by using response surface methodology (RSM). The extraction conditions associated with anthocyanin yield, including extraction solvent, liquid-to-solid rate, temperature and extraction time, are discussed. The optimal conditions obtained by RSM for UAE from mulberry include 63.8% methanol contains 1% (v/v) trifluoroacetic acid (TFA), 43.2 °C temperature, 23.8 (v/w) liquid-to-solid ratio, and 40 min time for the maximum yield (64.70 ± 0.45 mg/g). The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.

Show MeSH
Related in: MedlinePlus