Limits...
Knockdown of Akt sensitizes osteosarcoma cells to apoptosis induced by cisplatin treatment.

Zhang G, Li M, Zhu X, Bai Y, Yang C - Int J Mol Sci (2011)

Bottom Line: Reduced expression of Akt2 did not directly inhibit the growth rate of the transfected cells; however, it significantly increased their sensitivity to cisplatin.Knockdown of Akt2, together with cisplatin treatment, promoted the expression of p53 up-regulated modulator of apoptosis (PUMA).It is possible that the augmentation of cisplatin cytotoxicity may be mediated by PUMA activation.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; E-Mails: Zhangguoyou7@gmail.com (G.Z.); zhgych@yahoo.com.cn (X.Z.); baiyushu@21cn.com (Y.B.); changwei_y@yahoo.com.cn (W.Y.).

ABSTRACT
Akt plays an important role in the inhibition of apoptosis induced by chemotherapy and other stimuli. We therefore investigated if knockdown of Akt2 promoted drug-induced apoptosis in cultured osteosarcoma cells in vitro. SAOS-2 cells were transfected with Akt2 siRNA. The sensitivity of the transformed cell line to the chemotherapeutic drug cisplatin was assessed. Reduced expression of Akt2 did not directly inhibit the growth rate of the transfected cells; however, it significantly increased their sensitivity to cisplatin. Knockdown of Akt2, together with cisplatin treatment, promoted the expression of p53 up-regulated modulator of apoptosis (PUMA). It is possible that the augmentation of cisplatin cytotoxicity may be mediated by PUMA activation. The results of this study suggest that knockdown of Akt2 expression may have therapeutic applications in enhancing the efficacy of chemotherapy in patients with osteosarcoma.

Show MeSH

Related in: MedlinePlus

Immunohistrology analysis of levels of Slug protein in Akt 1, Akt2 and Akt3 in SAOS-2 cells. Significant Akt2 staining was seen in the cytoplasm. No obvious Akt1 and Akt3 staining was shown (BAC × 200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC3116170&req=5

f1-ijms-12-02994: Immunohistrology analysis of levels of Slug protein in Akt 1, Akt2 and Akt3 in SAOS-2 cells. Significant Akt2 staining was seen in the cytoplasm. No obvious Akt1 and Akt3 staining was shown (BAC × 200).

Mentions: Figure 1 shows the representative expression patterns of Akt1, Akt2 and Akt3 in SAOS-2 cells. Significant Akt2 staining was seen in the cytoplasm. No obvious Akt1 and Akt3 staining was shown in the SAOS-2 cells. In the present study, Akt2 was used for further study.


Knockdown of Akt sensitizes osteosarcoma cells to apoptosis induced by cisplatin treatment.

Zhang G, Li M, Zhu X, Bai Y, Yang C - Int J Mol Sci (2011)

Immunohistrology analysis of levels of Slug protein in Akt 1, Akt2 and Akt3 in SAOS-2 cells. Significant Akt2 staining was seen in the cytoplasm. No obvious Akt1 and Akt3 staining was shown (BAC × 200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116170&req=5

f1-ijms-12-02994: Immunohistrology analysis of levels of Slug protein in Akt 1, Akt2 and Akt3 in SAOS-2 cells. Significant Akt2 staining was seen in the cytoplasm. No obvious Akt1 and Akt3 staining was shown (BAC × 200).
Mentions: Figure 1 shows the representative expression patterns of Akt1, Akt2 and Akt3 in SAOS-2 cells. Significant Akt2 staining was seen in the cytoplasm. No obvious Akt1 and Akt3 staining was shown in the SAOS-2 cells. In the present study, Akt2 was used for further study.

Bottom Line: Reduced expression of Akt2 did not directly inhibit the growth rate of the transfected cells; however, it significantly increased their sensitivity to cisplatin.Knockdown of Akt2, together with cisplatin treatment, promoted the expression of p53 up-regulated modulator of apoptosis (PUMA).It is possible that the augmentation of cisplatin cytotoxicity may be mediated by PUMA activation.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; E-Mails: Zhangguoyou7@gmail.com (G.Z.); zhgych@yahoo.com.cn (X.Z.); baiyushu@21cn.com (Y.B.); changwei_y@yahoo.com.cn (W.Y.).

ABSTRACT
Akt plays an important role in the inhibition of apoptosis induced by chemotherapy and other stimuli. We therefore investigated if knockdown of Akt2 promoted drug-induced apoptosis in cultured osteosarcoma cells in vitro. SAOS-2 cells were transfected with Akt2 siRNA. The sensitivity of the transformed cell line to the chemotherapeutic drug cisplatin was assessed. Reduced expression of Akt2 did not directly inhibit the growth rate of the transfected cells; however, it significantly increased their sensitivity to cisplatin. Knockdown of Akt2, together with cisplatin treatment, promoted the expression of p53 up-regulated modulator of apoptosis (PUMA). It is possible that the augmentation of cisplatin cytotoxicity may be mediated by PUMA activation. The results of this study suggest that knockdown of Akt2 expression may have therapeutic applications in enhancing the efficacy of chemotherapy in patients with osteosarcoma.

Show MeSH
Related in: MedlinePlus