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Polyphenolic contents and antioxidant potential of stem bark extracts from Jatropha curcas (Linn).

Igbinosa OO, Igbinosa IH, Chigor VN, Uzunuigbe OE, Oyedemi SO, Odjadjare EE, Okoh AI, Igbinosa EO - Int J Mol Sci (2011)

Bottom Line: The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods.The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards.Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

View Article: PubMed Central - PubMed

Affiliation: Drexel University Medical School, St. Peter's University Hospital, New Brunswick, NJ 08901, USA; E-Mail: uyi80@yahoo.com.

ABSTRACT
We assessed the polyphenolic contents and antioxidant potential of the aqueous, ethanol and methanol stem bark extracts of Jatropha curcas. The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods. The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards. The concentrations of different classes of phenolic compounds were higher in methanol and ethanol extracts compared to aqueous extracts. There was correlation between total phenol, total flavonoids, total flavonol and total proanthocyanidins (r = 0.996, 0.978, 0.908, and 0.985) respectively. There was correlations between the amount of phenolic compounds and percentage inhibition of DPPH radicals scavenging activity of the extract (r = 0.98). Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

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Percentage inhibition of hydrogen peroxide scavenging activities of different extracts of J. curcas.
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f6-ijms-12-02958: Percentage inhibition of hydrogen peroxide scavenging activities of different extracts of J. curcas.

Mentions: The scavenging ability of aqueous, ethanol and methanol extracts (compared to BHT as standard) on hydrogen peroxide is shown in Figure 6. The percentage inhibition of hydrogen peroxide of BHT, methanol, aqueous and ethanol extracts of J. curcas at 0.8 and 1.0 mg/mL was found to be 95.82%, 89.93%, 83.92% and 82.19% respectively and 97.92%, 90.50%, 88.50% and 85.67% respectively. The extracts of J. curcas were capable of scavenging hydrogen peroxide in a concentration dependent manner and have a stronger hydrogen peroxide scavenging activity as compared with BHT. Hydrogen peroxide itself is not very reactive, but it could be toxic to cells because of its ability to penetrate biological membrance. As a result it may give rise to hydroxyl radical production in the cells [31]. Scavenging of H2O2 by the plant extracts may be attributed to their phenolics, which donate electron to H2O2, thus reducing it to water.


Polyphenolic contents and antioxidant potential of stem bark extracts from Jatropha curcas (Linn).

Igbinosa OO, Igbinosa IH, Chigor VN, Uzunuigbe OE, Oyedemi SO, Odjadjare EE, Okoh AI, Igbinosa EO - Int J Mol Sci (2011)

Percentage inhibition of hydrogen peroxide scavenging activities of different extracts of J. curcas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116167&req=5

f6-ijms-12-02958: Percentage inhibition of hydrogen peroxide scavenging activities of different extracts of J. curcas.
Mentions: The scavenging ability of aqueous, ethanol and methanol extracts (compared to BHT as standard) on hydrogen peroxide is shown in Figure 6. The percentage inhibition of hydrogen peroxide of BHT, methanol, aqueous and ethanol extracts of J. curcas at 0.8 and 1.0 mg/mL was found to be 95.82%, 89.93%, 83.92% and 82.19% respectively and 97.92%, 90.50%, 88.50% and 85.67% respectively. The extracts of J. curcas were capable of scavenging hydrogen peroxide in a concentration dependent manner and have a stronger hydrogen peroxide scavenging activity as compared with BHT. Hydrogen peroxide itself is not very reactive, but it could be toxic to cells because of its ability to penetrate biological membrance. As a result it may give rise to hydroxyl radical production in the cells [31]. Scavenging of H2O2 by the plant extracts may be attributed to their phenolics, which donate electron to H2O2, thus reducing it to water.

Bottom Line: The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods.The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards.Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

View Article: PubMed Central - PubMed

Affiliation: Drexel University Medical School, St. Peter's University Hospital, New Brunswick, NJ 08901, USA; E-Mail: uyi80@yahoo.com.

ABSTRACT
We assessed the polyphenolic contents and antioxidant potential of the aqueous, ethanol and methanol stem bark extracts of Jatropha curcas. The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods. The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards. The concentrations of different classes of phenolic compounds were higher in methanol and ethanol extracts compared to aqueous extracts. There was correlation between total phenol, total flavonoids, total flavonol and total proanthocyanidins (r = 0.996, 0.978, 0.908, and 0.985) respectively. There was correlations between the amount of phenolic compounds and percentage inhibition of DPPH radicals scavenging activity of the extract (r = 0.98). Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

Show MeSH
Related in: MedlinePlus