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Polyphenolic contents and antioxidant potential of stem bark extracts from Jatropha curcas (Linn).

Igbinosa OO, Igbinosa IH, Chigor VN, Uzunuigbe OE, Oyedemi SO, Odjadjare EE, Okoh AI, Igbinosa EO - Int J Mol Sci (2011)

Bottom Line: The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods.The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards.Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

View Article: PubMed Central - PubMed

Affiliation: Drexel University Medical School, St. Peter's University Hospital, New Brunswick, NJ 08901, USA; E-Mail: uyi80@yahoo.com.

ABSTRACT
We assessed the polyphenolic contents and antioxidant potential of the aqueous, ethanol and methanol stem bark extracts of Jatropha curcas. The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods. The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards. The concentrations of different classes of phenolic compounds were higher in methanol and ethanol extracts compared to aqueous extracts. There was correlation between total phenol, total flavonoids, total flavonol and total proanthocyanidins (r = 0.996, 0.978, 0.908, and 0.985) respectively. There was correlations between the amount of phenolic compounds and percentage inhibition of DPPH radicals scavenging activity of the extract (r = 0.98). Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

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Percentage inhibition of nitric oxide scavenging activities of different extracts of J. curcas.
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f5-ijms-12-02958: Percentage inhibition of nitric oxide scavenging activities of different extracts of J. curcas.

Mentions: The extracts showed strong inhibitory activities with percentage inhibition of nitric oxide at 1.0 mg/mL the highest concentration of BHT, methanol, aqueous and ethanol extracts of J. curcas exhibit 92.95%, 80.50%, 75.89%, and 70.50% respectively, as shown in Figure 5. Nitric oxide is a reactive free radical produced from sodium nitroprusside in an aqueous solution at physiological pH and reacts with oxygen in the reaction to form nitrite. The extracts inhibit nitrite formation by directly competing with oxygen in the reaction with nitric oxide and other nitrogen oxides such as NO3, and N2O3 [29]. The level of nitric oxide was significantly (P < 0.01) decreased in this study by the plant extracts. The previous report on the anti-inflammatory role of nitric oxide [30] could support the use of J. curcas for treatment of inflammation and healing of wound.


Polyphenolic contents and antioxidant potential of stem bark extracts from Jatropha curcas (Linn).

Igbinosa OO, Igbinosa IH, Chigor VN, Uzunuigbe OE, Oyedemi SO, Odjadjare EE, Okoh AI, Igbinosa EO - Int J Mol Sci (2011)

Percentage inhibition of nitric oxide scavenging activities of different extracts of J. curcas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116167&req=5

f5-ijms-12-02958: Percentage inhibition of nitric oxide scavenging activities of different extracts of J. curcas.
Mentions: The extracts showed strong inhibitory activities with percentage inhibition of nitric oxide at 1.0 mg/mL the highest concentration of BHT, methanol, aqueous and ethanol extracts of J. curcas exhibit 92.95%, 80.50%, 75.89%, and 70.50% respectively, as shown in Figure 5. Nitric oxide is a reactive free radical produced from sodium nitroprusside in an aqueous solution at physiological pH and reacts with oxygen in the reaction to form nitrite. The extracts inhibit nitrite formation by directly competing with oxygen in the reaction with nitric oxide and other nitrogen oxides such as NO3, and N2O3 [29]. The level of nitric oxide was significantly (P < 0.01) decreased in this study by the plant extracts. The previous report on the anti-inflammatory role of nitric oxide [30] could support the use of J. curcas for treatment of inflammation and healing of wound.

Bottom Line: The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods.The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards.Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

View Article: PubMed Central - PubMed

Affiliation: Drexel University Medical School, St. Peter's University Hospital, New Brunswick, NJ 08901, USA; E-Mail: uyi80@yahoo.com.

ABSTRACT
We assessed the polyphenolic contents and antioxidant potential of the aqueous, ethanol and methanol stem bark extracts of Jatropha curcas. The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods. The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards. The concentrations of different classes of phenolic compounds were higher in methanol and ethanol extracts compared to aqueous extracts. There was correlation between total phenol, total flavonoids, total flavonol and total proanthocyanidins (r = 0.996, 0.978, 0.908, and 0.985) respectively. There was correlations between the amount of phenolic compounds and percentage inhibition of DPPH radicals scavenging activity of the extract (r = 0.98). Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

Show MeSH
Related in: MedlinePlus