Limits...
Polyphenolic contents and antioxidant potential of stem bark extracts from Jatropha curcas (Linn).

Igbinosa OO, Igbinosa IH, Chigor VN, Uzunuigbe OE, Oyedemi SO, Odjadjare EE, Okoh AI, Igbinosa EO - Int J Mol Sci (2011)

Bottom Line: The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods.The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards.Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

View Article: PubMed Central - PubMed

Affiliation: Drexel University Medical School, St. Peter's University Hospital, New Brunswick, NJ 08901, USA; E-Mail: uyi80@yahoo.com.

ABSTRACT
We assessed the polyphenolic contents and antioxidant potential of the aqueous, ethanol and methanol stem bark extracts of Jatropha curcas. The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods. The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards. The concentrations of different classes of phenolic compounds were higher in methanol and ethanol extracts compared to aqueous extracts. There was correlation between total phenol, total flavonoids, total flavonol and total proanthocyanidins (r = 0.996, 0.978, 0.908, and 0.985) respectively. There was correlations between the amount of phenolic compounds and percentage inhibition of DPPH radicals scavenging activity of the extract (r = 0.98). Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

Show MeSH

Related in: MedlinePlus

Percentage inhibition of superoxide anion radical scavenging activities of different extracts of J. curcas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC3116167&req=5

f4-ijms-12-02958: Percentage inhibition of superoxide anion radical scavenging activities of different extracts of J. curcas.

Mentions: The percentage inhibition of superoxide anion by the extracts and standard drugs is shown in Figure 4. All solvents extracts of J. curcas have strong superoxide radical scavenging activity and exhibited higher superoxide radical scavenging activity when compared with BHT. The percentage inhibition of superoxide radical of BHT, methanol, aqueous and ethanol extracts of J. curcas at 1.0 mg/mL was found to be 85.69%, 80.29%, 79.23% and 75.92% respectively. All activity followed a concentration dependent manner and compared favorably well with the standard BHT. In the phenazine methosulphate/nicotinamide adenine dinucleotide-nitroblue tetrazolium (PMS/NADH-NBT) system, superoxide anion derived from dissolved oxygen by PMS/NADH coupling reaction reduces NBT. The decrease of absorbance at 560 nm with antioxidants thus indicates the consumption of superoxide anion in the reaction mixture. Superoxide anion radical is one of the strongest reactive oxygen species among the free radicals that are generated [28]. The scavenging activity of this radical by the plant extract suggests its potent scavenger of superoxide radical.


Polyphenolic contents and antioxidant potential of stem bark extracts from Jatropha curcas (Linn).

Igbinosa OO, Igbinosa IH, Chigor VN, Uzunuigbe OE, Oyedemi SO, Odjadjare EE, Okoh AI, Igbinosa EO - Int J Mol Sci (2011)

Percentage inhibition of superoxide anion radical scavenging activities of different extracts of J. curcas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116167&req=5

f4-ijms-12-02958: Percentage inhibition of superoxide anion radical scavenging activities of different extracts of J. curcas.
Mentions: The percentage inhibition of superoxide anion by the extracts and standard drugs is shown in Figure 4. All solvents extracts of J. curcas have strong superoxide radical scavenging activity and exhibited higher superoxide radical scavenging activity when compared with BHT. The percentage inhibition of superoxide radical of BHT, methanol, aqueous and ethanol extracts of J. curcas at 1.0 mg/mL was found to be 85.69%, 80.29%, 79.23% and 75.92% respectively. All activity followed a concentration dependent manner and compared favorably well with the standard BHT. In the phenazine methosulphate/nicotinamide adenine dinucleotide-nitroblue tetrazolium (PMS/NADH-NBT) system, superoxide anion derived from dissolved oxygen by PMS/NADH coupling reaction reduces NBT. The decrease of absorbance at 560 nm with antioxidants thus indicates the consumption of superoxide anion in the reaction mixture. Superoxide anion radical is one of the strongest reactive oxygen species among the free radicals that are generated [28]. The scavenging activity of this radical by the plant extract suggests its potent scavenger of superoxide radical.

Bottom Line: The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods.The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards.Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

View Article: PubMed Central - PubMed

Affiliation: Drexel University Medical School, St. Peter's University Hospital, New Brunswick, NJ 08901, USA; E-Mail: uyi80@yahoo.com.

ABSTRACT
We assessed the polyphenolic contents and antioxidant potential of the aqueous, ethanol and methanol stem bark extracts of Jatropha curcas. The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods. The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards. The concentrations of different classes of phenolic compounds were higher in methanol and ethanol extracts compared to aqueous extracts. There was correlation between total phenol, total flavonoids, total flavonol and total proanthocyanidins (r = 0.996, 0.978, 0.908, and 0.985) respectively. There was correlations between the amount of phenolic compounds and percentage inhibition of DPPH radicals scavenging activity of the extract (r = 0.98). Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

Show MeSH
Related in: MedlinePlus