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Polyphenolic contents and antioxidant potential of stem bark extracts from Jatropha curcas (Linn).

Igbinosa OO, Igbinosa IH, Chigor VN, Uzunuigbe OE, Oyedemi SO, Odjadjare EE, Okoh AI, Igbinosa EO - Int J Mol Sci (2011)

Bottom Line: The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods.The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards.Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

View Article: PubMed Central - PubMed

Affiliation: Drexel University Medical School, St. Peter's University Hospital, New Brunswick, NJ 08901, USA; E-Mail: uyi80@yahoo.com.

ABSTRACT
We assessed the polyphenolic contents and antioxidant potential of the aqueous, ethanol and methanol stem bark extracts of Jatropha curcas. The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods. The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards. The concentrations of different classes of phenolic compounds were higher in methanol and ethanol extracts compared to aqueous extracts. There was correlation between total phenol, total flavonoids, total flavonol and total proanthocyanidins (r = 0.996, 0.978, 0.908, and 0.985) respectively. There was correlations between the amount of phenolic compounds and percentage inhibition of DPPH radicals scavenging activity of the extract (r = 0.98). Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

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Total ferric reductive potential of the different solvents extract of J. curcas.
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f3-ijms-12-02958: Total ferric reductive potential of the different solvents extract of J. curcas.

Mentions: The reducing power of J. curcas extracts and the reference compounds increased with increasing concentration (Figure 3). The antioxidant potentials of the plant extracts was estimated from their ability to reduce Fe3+ to Fe2+. This was observed from yellow color of the test solution that changed to various shades of green and blue depending on the concentration of the plant extracts. The reducing value of the plant extracts was significantly lower than that of BHT and ascorbic acids, used as reference compounds in this study (Figure 3). There was significant difference in reducing activities between the plant extracts, BHT and ascorbic acids. However, reducing power of BHT and ascorbic acids was significantly higher (P < 0.01) than that of the plant extracts. The reducing power of the extracts, BHT and ascorbic acid increased with increasing concentration (Figure 3) in the following order, ascorbic acid > BHT > methanol > ethanol > aqueous extracts. Antioxidant activity has been shown to be related to the development of reductones, which are terminators of free radical chain reactors [5]. The presence of reductants such as antioxidant substances in the samples causes a reduction of the Fe3+ to Fe2+ form. Therefore, the ability of a compound to transfer electron is a significant indicator of its potential as an antioxidant [25]. The results showed that good correlations exist between reducing power, DPPH radical scavenging activity and total phenolics content of the extract. This indicates that the antioxidant compounds are electron donors and could reduce the oxidized intermediate of lipid peroxidation processes; thus acting as primary and secondary antioxidants [26,27].


Polyphenolic contents and antioxidant potential of stem bark extracts from Jatropha curcas (Linn).

Igbinosa OO, Igbinosa IH, Chigor VN, Uzunuigbe OE, Oyedemi SO, Odjadjare EE, Okoh AI, Igbinosa EO - Int J Mol Sci (2011)

Total ferric reductive potential of the different solvents extract of J. curcas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116167&req=5

f3-ijms-12-02958: Total ferric reductive potential of the different solvents extract of J. curcas.
Mentions: The reducing power of J. curcas extracts and the reference compounds increased with increasing concentration (Figure 3). The antioxidant potentials of the plant extracts was estimated from their ability to reduce Fe3+ to Fe2+. This was observed from yellow color of the test solution that changed to various shades of green and blue depending on the concentration of the plant extracts. The reducing value of the plant extracts was significantly lower than that of BHT and ascorbic acids, used as reference compounds in this study (Figure 3). There was significant difference in reducing activities between the plant extracts, BHT and ascorbic acids. However, reducing power of BHT and ascorbic acids was significantly higher (P < 0.01) than that of the plant extracts. The reducing power of the extracts, BHT and ascorbic acid increased with increasing concentration (Figure 3) in the following order, ascorbic acid > BHT > methanol > ethanol > aqueous extracts. Antioxidant activity has been shown to be related to the development of reductones, which are terminators of free radical chain reactors [5]. The presence of reductants such as antioxidant substances in the samples causes a reduction of the Fe3+ to Fe2+ form. Therefore, the ability of a compound to transfer electron is a significant indicator of its potential as an antioxidant [25]. The results showed that good correlations exist between reducing power, DPPH radical scavenging activity and total phenolics content of the extract. This indicates that the antioxidant compounds are electron donors and could reduce the oxidized intermediate of lipid peroxidation processes; thus acting as primary and secondary antioxidants [26,27].

Bottom Line: The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods.The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards.Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

View Article: PubMed Central - PubMed

Affiliation: Drexel University Medical School, St. Peter's University Hospital, New Brunswick, NJ 08901, USA; E-Mail: uyi80@yahoo.com.

ABSTRACT
We assessed the polyphenolic contents and antioxidant potential of the aqueous, ethanol and methanol stem bark extracts of Jatropha curcas. The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods. The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards. The concentrations of different classes of phenolic compounds were higher in methanol and ethanol extracts compared to aqueous extracts. There was correlation between total phenol, total flavonoids, total flavonol and total proanthocyanidins (r = 0.996, 0.978, 0.908, and 0.985) respectively. There was correlations between the amount of phenolic compounds and percentage inhibition of DPPH radicals scavenging activity of the extract (r = 0.98). Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

Show MeSH
Related in: MedlinePlus