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Polyphenolic contents and antioxidant potential of stem bark extracts from Jatropha curcas (Linn).

Igbinosa OO, Igbinosa IH, Chigor VN, Uzunuigbe OE, Oyedemi SO, Odjadjare EE, Okoh AI, Igbinosa EO - Int J Mol Sci (2011)

Bottom Line: The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods.The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards.Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

View Article: PubMed Central - PubMed

Affiliation: Drexel University Medical School, St. Peter's University Hospital, New Brunswick, NJ 08901, USA; E-Mail: uyi80@yahoo.com.

ABSTRACT
We assessed the polyphenolic contents and antioxidant potential of the aqueous, ethanol and methanol stem bark extracts of Jatropha curcas. The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods. The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards. The concentrations of different classes of phenolic compounds were higher in methanol and ethanol extracts compared to aqueous extracts. There was correlation between total phenol, total flavonoids, total flavonol and total proanthocyanidins (r = 0.996, 0.978, 0.908, and 0.985) respectively. There was correlations between the amount of phenolic compounds and percentage inhibition of DPPH radicals scavenging activity of the extract (r = 0.98). Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

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ABTS+ radical scavenging activities of the different extracts of J. curcas.
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f2-ijms-12-02958: ABTS+ radical scavenging activities of the different extracts of J. curcas.

Mentions: The percentage inhibition of ABTS radical by the plant extracts was concentration dependent. There was increased in ABTS radical scavenging activity with increasing concentration at different solvent extraction used in this study (Figure 2). At a concentration of 1.0 mg/mL, the percentage inhibition of methanol extract (89.0%); ethanol extract (87.78%) and aqueous extract (86.8%) showed similar trend but significantly different when compared with that of BHT (96.9%). High concentrations of the extract have been reported to be more effective in quenching free radical in the system [23]. The scavenging activity of ABTS+ by the plant extract was found to be remarkably high. The finding obtained in this study differ from previous study by Wang et al. [24] who reported that compounds which exhibit ABTS+ scavenging activity may not posses DPPH scavenging activity. The extracts were able to inhibit both DPPH and ABTS radicals with similar trend. This implies that the plant extracts could be useful for treating radical-related pathological damages especially at higher concentrations.


Polyphenolic contents and antioxidant potential of stem bark extracts from Jatropha curcas (Linn).

Igbinosa OO, Igbinosa IH, Chigor VN, Uzunuigbe OE, Oyedemi SO, Odjadjare EE, Okoh AI, Igbinosa EO - Int J Mol Sci (2011)

ABTS+ radical scavenging activities of the different extracts of J. curcas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116167&req=5

f2-ijms-12-02958: ABTS+ radical scavenging activities of the different extracts of J. curcas.
Mentions: The percentage inhibition of ABTS radical by the plant extracts was concentration dependent. There was increased in ABTS radical scavenging activity with increasing concentration at different solvent extraction used in this study (Figure 2). At a concentration of 1.0 mg/mL, the percentage inhibition of methanol extract (89.0%); ethanol extract (87.78%) and aqueous extract (86.8%) showed similar trend but significantly different when compared with that of BHT (96.9%). High concentrations of the extract have been reported to be more effective in quenching free radical in the system [23]. The scavenging activity of ABTS+ by the plant extract was found to be remarkably high. The finding obtained in this study differ from previous study by Wang et al. [24] who reported that compounds which exhibit ABTS+ scavenging activity may not posses DPPH scavenging activity. The extracts were able to inhibit both DPPH and ABTS radicals with similar trend. This implies that the plant extracts could be useful for treating radical-related pathological damages especially at higher concentrations.

Bottom Line: The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods.The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards.Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

View Article: PubMed Central - PubMed

Affiliation: Drexel University Medical School, St. Peter's University Hospital, New Brunswick, NJ 08901, USA; E-Mail: uyi80@yahoo.com.

ABSTRACT
We assessed the polyphenolic contents and antioxidant potential of the aqueous, ethanol and methanol stem bark extracts of Jatropha curcas. The total phenol, flavonoids, flavonols and proanthocyanidin contents of the extracts were evaluated to determine their effect on the antioxidant property of this plant, using standard phytochemical methods. The antioxidant and free radical scavenging activity of ethanol, methanol and aqueous extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing, nitric oxide (NO), superoxide anion, (O(2) (-)) and hydrogen peroxide (H(2)O(2)) using spectroscopic methods and results were compared with that of butylated hydroxyl toluene (BHT) and ascorbic acid as standards. The concentrations of different classes of phenolic compounds were higher in methanol and ethanol extracts compared to aqueous extracts. There was correlation between total phenol, total flavonoids, total flavonol and total proanthocyanidins (r = 0.996, 0.978, 0.908, and 0.985) respectively. There was correlations between the amount of phenolic compounds and percentage inhibition of DPPH radicals scavenging activity of the extract (r = 0.98). Findings from the present study indicated that J. curcas is a potential source of natural antioxidants and may be a good candidate for pharmaceutical plant based products.

Show MeSH
Related in: MedlinePlus