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D-(+)-galactose-conjugated single-walled carbon nanotubes as new chemical probes for electrochemical biosensors for the cancer marker galectin-3.

Park YK, Bold B, Lee WK, Jeon MH, An KH, Jeong SY, Shim YK - Int J Mol Sci (2011)

Bottom Line: To investigate the binding of galectin-3 to the d-(+)-galactose-conjugated SWCNTs, an electrochemical biosensor was fabricated by using molybdenum electrodes.The binding affinities of the conjugated SWCNTs to galectin-3 were quantified using electrochemical sensitivity measurements based on the differences in resistance together with typical I-V characterization.The electrochemical sensitivity measurements of the d-(+)-galactose-conjugated SWCNTs differed significantly between the samples with and without galectin-3.

View Article: PubMed Central - PubMed

Affiliation: Department of Nano System Engineering, Inje University, Gimhae, 612-749, Korea; E-Mails: immergreen@dreamwiz.com (Y.K.P.); bayarma_sbmt@yahoo.com (B.B.); wlee@inje.ac.kr (W.K.L.); mjeon@inje.ac.kr (M.H.J.).

ABSTRACT
D-(+)-Galactose-conjugated single-walled carbon nanotubes (SWCNTs) were synthesized for use as biosensors to detect the cancer marker galectin-3. To investigate the binding of galectin-3 to the d-(+)-galactose-conjugated SWCNTs, an electrochemical biosensor was fabricated by using molybdenum electrodes. The binding affinities of the conjugated SWCNTs to galectin-3 were quantified using electrochemical sensitivity measurements based on the differences in resistance together with typical I-V characterization. The electrochemical sensitivity measurements of the d-(+)-galactose-conjugated SWCNTs differed significantly between the samples with and without galectin-3. This indicates that d-(+)-galactose-conjugated SWCNTs are potentially useful electrochemical biosensors for the detection of cancer marker galectin-3.

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Related in: MedlinePlus

Images and analysis of SWCNTS after introducing d-(+)-galactose. (a) FE-SEM image, scale bar 100 nm; (b) HR-TEM image, scale bar 0.5 μm; (c) d-band in FT-Raman; (d) XPS C1S.
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f1-ijms-12-02946: Images and analysis of SWCNTS after introducing d-(+)-galactose. (a) FE-SEM image, scale bar 100 nm; (b) HR-TEM image, scale bar 0.5 μm; (c) d-band in FT-Raman; (d) XPS C1S.

Mentions: The FE-SEM and HR-TEM observations showed that the structure of the SWCNTs did not change after the reaction with d-(+)-galactose on SWCNTs functionalized with –COCl (Figure 1a and 1b). As shown in Figure 1c, the d-band related to sp3 was markedly increased due to sp3 bonding of d-(+)-galactose immobilized on the surface of the SWCNTs. After the reaction with d-(+)-galactose, the peak of sp3 bonding was increased markedly at 285.2 eV in the XPS C1S analysis (Figure 1d).


D-(+)-galactose-conjugated single-walled carbon nanotubes as new chemical probes for electrochemical biosensors for the cancer marker galectin-3.

Park YK, Bold B, Lee WK, Jeon MH, An KH, Jeong SY, Shim YK - Int J Mol Sci (2011)

Images and analysis of SWCNTS after introducing d-(+)-galactose. (a) FE-SEM image, scale bar 100 nm; (b) HR-TEM image, scale bar 0.5 μm; (c) d-band in FT-Raman; (d) XPS C1S.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116166&req=5

f1-ijms-12-02946: Images and analysis of SWCNTS after introducing d-(+)-galactose. (a) FE-SEM image, scale bar 100 nm; (b) HR-TEM image, scale bar 0.5 μm; (c) d-band in FT-Raman; (d) XPS C1S.
Mentions: The FE-SEM and HR-TEM observations showed that the structure of the SWCNTs did not change after the reaction with d-(+)-galactose on SWCNTs functionalized with –COCl (Figure 1a and 1b). As shown in Figure 1c, the d-band related to sp3 was markedly increased due to sp3 bonding of d-(+)-galactose immobilized on the surface of the SWCNTs. After the reaction with d-(+)-galactose, the peak of sp3 bonding was increased markedly at 285.2 eV in the XPS C1S analysis (Figure 1d).

Bottom Line: To investigate the binding of galectin-3 to the d-(+)-galactose-conjugated SWCNTs, an electrochemical biosensor was fabricated by using molybdenum electrodes.The binding affinities of the conjugated SWCNTs to galectin-3 were quantified using electrochemical sensitivity measurements based on the differences in resistance together with typical I-V characterization.The electrochemical sensitivity measurements of the d-(+)-galactose-conjugated SWCNTs differed significantly between the samples with and without galectin-3.

View Article: PubMed Central - PubMed

Affiliation: Department of Nano System Engineering, Inje University, Gimhae, 612-749, Korea; E-Mails: immergreen@dreamwiz.com (Y.K.P.); bayarma_sbmt@yahoo.com (B.B.); wlee@inje.ac.kr (W.K.L.); mjeon@inje.ac.kr (M.H.J.).

ABSTRACT
D-(+)-Galactose-conjugated single-walled carbon nanotubes (SWCNTs) were synthesized for use as biosensors to detect the cancer marker galectin-3. To investigate the binding of galectin-3 to the d-(+)-galactose-conjugated SWCNTs, an electrochemical biosensor was fabricated by using molybdenum electrodes. The binding affinities of the conjugated SWCNTs to galectin-3 were quantified using electrochemical sensitivity measurements based on the differences in resistance together with typical I-V characterization. The electrochemical sensitivity measurements of the d-(+)-galactose-conjugated SWCNTs differed significantly between the samples with and without galectin-3. This indicates that d-(+)-galactose-conjugated SWCNTs are potentially useful electrochemical biosensors for the detection of cancer marker galectin-3.

Show MeSH
Related in: MedlinePlus