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A newly isolated thermostable lipase from Bacillus sp.

Shariff FM, Rahman RN, Basri M, Salleh AB - Int J Mol Sci (2011)

Bottom Line: Recombinant lipase was successfully overexpressed with a 178-fold increase in activity compared to crude native L2 lipase.The purified lipase was found to be reactive at a temperature range of 55-80 °C and at a pH of 6-10.The optimum activity was found to be at 70 °C and pH 9.

View Article: PubMed Central - PubMed

Affiliation: Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; E-Mails: ferrol2506@gmail.com (F.M.S.); abubakar@biotech.upm.edu.my (A.B.S.).

ABSTRACT
A thermophilic lipolytic bacterium identified as Bacillus sp. L2 via 16S rDNA was previously isolated from a hot spring in Perak, Malaysia. Bacillus sp. L2 was confirmed to be in Group 5 of bacterial classification, a phylogenically and phenotypically coherent group of thermophilic bacilli displaying very high similarity among their 16S rRNA sequences (98.5-99.2%). Polymerase chain reaction (PCR) cloning of L2 lipase gene was conducted by using five different primers. Sequence analysis of the L2 lipase gene revealed an open reading frame (ORF) of 1251 bp that codes for 417 amino acids. The signal peptides consist of 28 amino acids. The mature protein is made of 388 amino acid residues. Recombinant lipase was successfully overexpressed with a 178-fold increase in activity compared to crude native L2 lipase. The recombinant L2 lipase (43.2 kDa) was purified to homogeneity in a single chromatography step. The purified lipase was found to be reactive at a temperature range of 55-80 °C and at a pH of 6-10. The L2 lipase had a melting temperature (Tm) of 59.04 °C when analyzed by circular dichroism (CD) spectroscopy studies. The optimum activity was found to be at 70 °C and pH 9. Lipase L2 was strongly inhibited by ethylenediaminetetraacetic acid (EDTA) (100%), whereas phenylmethylsulfonyl fluoride (PMSF), pepstatin-A, 2-mercaptoethanol and dithiothreitol (DTT) inhibited the enzyme by over 40%. The CD spectra of secondary structure analysis showed that the L2 lipase structure contained 38.6% α-helices, 2.2% ß-strands, 23.6% turns and 35.6% random conformations.

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Rooted phylogenetic tree showing the relationship of isolate Bacillus sp. L2 to other Bacillus and Geobacillus species with their accession numbers.
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f1-ijms-12-02917: Rooted phylogenetic tree showing the relationship of isolate Bacillus sp. L2 to other Bacillus and Geobacillus species with their accession numbers.

Mentions: Bacillus sp. L2 is an aerobic, non-motile Gram positive rod able to grow at a temperature range between 40–80 °C with an optimum growth at 70 °C and optimum pH of 7. The 16S rDNA nucleotide sequence of this strain has been deposited into GenBank data library and assigned the accession number AY 964643. BLAST results showed that Bacillus sp. L2 has highest homology with G. thermoglucosidiasus (AY 608988) with a significant value of 99% homology. From the phylogenetic tree and the G + C contents of 16S rRNA sequences, Bacillus sp. L2 was confirmed to be in Group 5 of bacterial classification (Figure 1). Group 5 is a phylogenically and phenotypically coherent group of thermophilic bacilli displaying very high similarity among their 16S rRNA sequences (98.5–99.2%) [5]. Bacillus sp. L2 was found to cluster closely with two other strains, G. caldoxylosilyticus (AJ 564613) and Bacillus sp. Ak1 (L 29507). The results from BLAST by NCBI also showed that Bacillus sp. L2 has 98% homology with these two strains, and also 99% homology with G. thermoglucosidiasus (AY 608988). However, from the phylogenetic tree, it was found that this Bacillus sp. L2 did not cluster closely with G. thermoglucosidiasus (AY 608988). So far, there are no reports on lipase production from either the G. thermoglucosidiasus or the G. caldoxylosilyticus species.


A newly isolated thermostable lipase from Bacillus sp.

Shariff FM, Rahman RN, Basri M, Salleh AB - Int J Mol Sci (2011)

Rooted phylogenetic tree showing the relationship of isolate Bacillus sp. L2 to other Bacillus and Geobacillus species with their accession numbers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3116164&req=5

f1-ijms-12-02917: Rooted phylogenetic tree showing the relationship of isolate Bacillus sp. L2 to other Bacillus and Geobacillus species with their accession numbers.
Mentions: Bacillus sp. L2 is an aerobic, non-motile Gram positive rod able to grow at a temperature range between 40–80 °C with an optimum growth at 70 °C and optimum pH of 7. The 16S rDNA nucleotide sequence of this strain has been deposited into GenBank data library and assigned the accession number AY 964643. BLAST results showed that Bacillus sp. L2 has highest homology with G. thermoglucosidiasus (AY 608988) with a significant value of 99% homology. From the phylogenetic tree and the G + C contents of 16S rRNA sequences, Bacillus sp. L2 was confirmed to be in Group 5 of bacterial classification (Figure 1). Group 5 is a phylogenically and phenotypically coherent group of thermophilic bacilli displaying very high similarity among their 16S rRNA sequences (98.5–99.2%) [5]. Bacillus sp. L2 was found to cluster closely with two other strains, G. caldoxylosilyticus (AJ 564613) and Bacillus sp. Ak1 (L 29507). The results from BLAST by NCBI also showed that Bacillus sp. L2 has 98% homology with these two strains, and also 99% homology with G. thermoglucosidiasus (AY 608988). However, from the phylogenetic tree, it was found that this Bacillus sp. L2 did not cluster closely with G. thermoglucosidiasus (AY 608988). So far, there are no reports on lipase production from either the G. thermoglucosidiasus or the G. caldoxylosilyticus species.

Bottom Line: Recombinant lipase was successfully overexpressed with a 178-fold increase in activity compared to crude native L2 lipase.The purified lipase was found to be reactive at a temperature range of 55-80 °C and at a pH of 6-10.The optimum activity was found to be at 70 °C and pH 9.

View Article: PubMed Central - PubMed

Affiliation: Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; E-Mails: ferrol2506@gmail.com (F.M.S.); abubakar@biotech.upm.edu.my (A.B.S.).

ABSTRACT
A thermophilic lipolytic bacterium identified as Bacillus sp. L2 via 16S rDNA was previously isolated from a hot spring in Perak, Malaysia. Bacillus sp. L2 was confirmed to be in Group 5 of bacterial classification, a phylogenically and phenotypically coherent group of thermophilic bacilli displaying very high similarity among their 16S rRNA sequences (98.5-99.2%). Polymerase chain reaction (PCR) cloning of L2 lipase gene was conducted by using five different primers. Sequence analysis of the L2 lipase gene revealed an open reading frame (ORF) of 1251 bp that codes for 417 amino acids. The signal peptides consist of 28 amino acids. The mature protein is made of 388 amino acid residues. Recombinant lipase was successfully overexpressed with a 178-fold increase in activity compared to crude native L2 lipase. The recombinant L2 lipase (43.2 kDa) was purified to homogeneity in a single chromatography step. The purified lipase was found to be reactive at a temperature range of 55-80 °C and at a pH of 6-10. The L2 lipase had a melting temperature (Tm) of 59.04 °C when analyzed by circular dichroism (CD) spectroscopy studies. The optimum activity was found to be at 70 °C and pH 9. Lipase L2 was strongly inhibited by ethylenediaminetetraacetic acid (EDTA) (100%), whereas phenylmethylsulfonyl fluoride (PMSF), pepstatin-A, 2-mercaptoethanol and dithiothreitol (DTT) inhibited the enzyme by over 40%. The CD spectra of secondary structure analysis showed that the L2 lipase structure contained 38.6% α-helices, 2.2% ß-strands, 23.6% turns and 35.6% random conformations.

Show MeSH