Combined characterization of microRNA and mRNA profiles delineates early differentiation pathways of CD133+ and CD34+ hematopoietic stem and progenitor cells.
Bottom Line: Using quantitative whole genome miRNA microarray and sequencing-based profiling, we found that between 109 (CD133(+) ) and 216 (CD34(-) CD133(-) ) miRNAs were expressed.In conclusion, the miRNAs expressed differentially between the CD133(+) and CD34(+) CD133(-) cells are involved in inhibition of differentiation, prevention of apoptosis, and cytoskeletal remodeling.These results are highly relevant for stem cell-based therapies with CD133(+) cells and delineate for the first time how the stem cell character of CD133(+) cells is defined by the expression of specific miRNAs.
Affiliation: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.Show MeSH
Mentions: Ninety-five miRNAs were sequenced with over 100 counts compared with 135 miRNAs detected onefold over background on all arrays. The overlap between the two groups was 81 miRNAs detected with both methods (Fig. 4A).
Affiliation: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.