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Combined characterization of microRNA and mRNA profiles delineates early differentiation pathways of CD133+ and CD34+ hematopoietic stem and progenitor cells.

Bissels U, Wild S, Tomiuk S, Hafner M, Scheel H, Mihailovic A, Choi YH, Tuschl T, Bosio A - Stem Cells (2011)

Bottom Line: Using quantitative whole genome miRNA microarray and sequencing-based profiling, we found that between 109 (CD133(+) ) and 216 (CD34(-) CD133(-) ) miRNAs were expressed.In conclusion, the miRNAs expressed differentially between the CD133(+) and CD34(+) CD133(-) cells are involved in inhibition of differentiation, prevention of apoptosis, and cytoskeletal remodeling.These results are highly relevant for stem cell-based therapies with CD133(+) cells and delineate for the first time how the stem cell character of CD133(+) cells is defined by the expression of specific miRNAs.

View Article: PubMed Central - PubMed

Affiliation: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.

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Average linkage cluster of miRNAs expressed in CD133+, CD34+CD133−, and CD34−CD133− (Neg) cells. The cluster was obtained from array hybridizations of different bone marrow subpopulations (n = 5) and leukapheresis samples (mPB, n = 2) versus universal reference. Those miRNAs were included where the net signal intensity of the sample miRNA was onefold over background in at least four of five donors in the CD133+ and/or CD34+CD133− cell population and where the miRNA was present in the UR. Neg +R: CD34−CD133− with RBCs; Neg −R: CD34−CD133− after RBC lysis. Log 2-transformed expression ratios are indicated from −3.0 (green) to 3.0 (red).
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fig02: Average linkage cluster of miRNAs expressed in CD133+, CD34+CD133−, and CD34−CD133− (Neg) cells. The cluster was obtained from array hybridizations of different bone marrow subpopulations (n = 5) and leukapheresis samples (mPB, n = 2) versus universal reference. Those miRNAs were included where the net signal intensity of the sample miRNA was onefold over background in at least four of five donors in the CD133+ and/or CD34+CD133− cell population and where the miRNA was present in the UR. Neg +R: CD34−CD133− with RBCs; Neg −R: CD34−CD133− after RBC lysis. Log 2-transformed expression ratios are indicated from −3.0 (green) to 3.0 (red).

Mentions: Next, we performed a cluster analysis of the miRNAs expressed in CD133+ and CD34+CD133− cells. The four subpopulations clustered separately as visualized in Figure 2 and revealed distinct miRNA signatures for the different cell populations. As expected, the similarity between CD133+ and CD34+CD133− cells was higher than between CD133+ and CD34−CD133− cells which mainly consist of granulocytes (Neg −R) or RBCs (Neg +R).


Combined characterization of microRNA and mRNA profiles delineates early differentiation pathways of CD133+ and CD34+ hematopoietic stem and progenitor cells.

Bissels U, Wild S, Tomiuk S, Hafner M, Scheel H, Mihailovic A, Choi YH, Tuschl T, Bosio A - Stem Cells (2011)

Average linkage cluster of miRNAs expressed in CD133+, CD34+CD133−, and CD34−CD133− (Neg) cells. The cluster was obtained from array hybridizations of different bone marrow subpopulations (n = 5) and leukapheresis samples (mPB, n = 2) versus universal reference. Those miRNAs were included where the net signal intensity of the sample miRNA was onefold over background in at least four of five donors in the CD133+ and/or CD34+CD133− cell population and where the miRNA was present in the UR. Neg +R: CD34−CD133− with RBCs; Neg −R: CD34−CD133− after RBC lysis. Log 2-transformed expression ratios are indicated from −3.0 (green) to 3.0 (red).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3116150&req=5

fig02: Average linkage cluster of miRNAs expressed in CD133+, CD34+CD133−, and CD34−CD133− (Neg) cells. The cluster was obtained from array hybridizations of different bone marrow subpopulations (n = 5) and leukapheresis samples (mPB, n = 2) versus universal reference. Those miRNAs were included where the net signal intensity of the sample miRNA was onefold over background in at least four of five donors in the CD133+ and/or CD34+CD133− cell population and where the miRNA was present in the UR. Neg +R: CD34−CD133− with RBCs; Neg −R: CD34−CD133− after RBC lysis. Log 2-transformed expression ratios are indicated from −3.0 (green) to 3.0 (red).
Mentions: Next, we performed a cluster analysis of the miRNAs expressed in CD133+ and CD34+CD133− cells. The four subpopulations clustered separately as visualized in Figure 2 and revealed distinct miRNA signatures for the different cell populations. As expected, the similarity between CD133+ and CD34+CD133− cells was higher than between CD133+ and CD34−CD133− cells which mainly consist of granulocytes (Neg −R) or RBCs (Neg +R).

Bottom Line: Using quantitative whole genome miRNA microarray and sequencing-based profiling, we found that between 109 (CD133(+) ) and 216 (CD34(-) CD133(-) ) miRNAs were expressed.In conclusion, the miRNAs expressed differentially between the CD133(+) and CD34(+) CD133(-) cells are involved in inhibition of differentiation, prevention of apoptosis, and cytoskeletal remodeling.These results are highly relevant for stem cell-based therapies with CD133(+) cells and delineate for the first time how the stem cell character of CD133(+) cells is defined by the expression of specific miRNAs.

View Article: PubMed Central - PubMed

Affiliation: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.

Show MeSH
Related in: MedlinePlus