Combined characterization of microRNA and mRNA profiles delineates early differentiation pathways of CD133+ and CD34+ hematopoietic stem and progenitor cells.
Bottom Line: Using quantitative whole genome miRNA microarray and sequencing-based profiling, we found that between 109 (CD133(+) ) and 216 (CD34(-) CD133(-) ) miRNAs were expressed.In conclusion, the miRNAs expressed differentially between the CD133(+) and CD34(+) CD133(-) cells are involved in inhibition of differentiation, prevention of apoptosis, and cytoskeletal remodeling.These results are highly relevant for stem cell-based therapies with CD133(+) cells and delineate for the first time how the stem cell character of CD133(+) cells is defined by the expression of specific miRNAs.
Affiliation: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.Show MeSH
Related in: MedlinePlus
Mentions: Next, we performed a cluster analysis of the miRNAs expressed in CD133+ and CD34+CD133− cells. The four subpopulations clustered separately as visualized in Figure 2 and revealed distinct miRNA signatures for the different cell populations. As expected, the similarity between CD133+ and CD34+CD133− cells was higher than between CD133+ and CD34−CD133− cells which mainly consist of granulocytes (Neg −R) or RBCs (Neg +R).
Affiliation: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.