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Microbial communities and chemosynthesis in yellowstone lake sublacustrine hydrothermal vent waters.

Yang T, Lyons S, Aguilar C, Cuhel R, Teske A - Front Microbiol (2011)

Bottom Line: They harbor distinct chemosynthetic bacterial communities, depending on temperature (16-110°C) and electron donor supply (H(2)S <1 to >100 μM; NH(3) <0.5 to >10 μM).Vents at lower temperatures yielded mostly phylotypes related to the mesophilic gammaproteobacterial sulfur oxidizer Thiovirga.In contrast, cool vent water with low chemosynthetic activity yielded predominantly phylotypes related to freshwater Actinobacterial clusters with a cosmopolitan distribution.

View Article: PubMed Central - PubMed

Affiliation: Department of Marine Sciences, University of North Carolina at Chapel Hill Chapel Hill, NC, USA.

ABSTRACT
Five sublacustrine thermal spring locations from 1 to 109 m water depth in Yellowstone Lake were surveyed by 16S ribosomal RNA gene sequencing in relation to their chemical composition and dark CO(2) fixation rates. They harbor distinct chemosynthetic bacterial communities, depending on temperature (16-110°C) and electron donor supply (H(2)S <1 to >100 μM; NH(3) <0.5 to >10 μM). Members of the Aquificales, most closely affiliated with the genus Sulfurihydrogenibium, are the most frequently recovered bacterial 16S rRNA gene phylotypes in the hottest samples; the detection of these thermophilic sulfur-oxidizing autotrophs coincided with maximal dark CO(2) fixation rates reaching near 9 μM C h(-1) at temperatures of 50-60°C. Vents at lower temperatures yielded mostly phylotypes related to the mesophilic gammaproteobacterial sulfur oxidizer Thiovirga. In contrast, cool vent water with low chemosynthetic activity yielded predominantly phylotypes related to freshwater Actinobacterial clusters with a cosmopolitan distribution.

No MeSH data available.


Photosynthesis-irradiance relationships at 15°C for surface waters overlying hydrothermal vent fields in Mary Bay (●), Stevenson Island (■), and West Thumb (▲).
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Figure 2: Photosynthesis-irradiance relationships at 15°C for surface waters overlying hydrothermal vent fields in Mary Bay (●), Stevenson Island (■), and West Thumb (▲).

Mentions: Photosynthesis-irradiance curves for surface samples from the three domains of Mary Bay, Stevenson Island, and West Thumb demonstrated light-saturable photosynthetic CO2 fixation (Figure 2). Light stimulated photosynthetic rates to 200 μmol PAR photons m−2 s−1, about 10% of full sunlight, above which rates remained constant at 0.14 ± 0.01 μM C h−1. Because this discussion concerns volumetric carbon fixation, the data were not normalized for varying algal biomass as chlorophyll a and are presented in the same units as chemosynthesis for direct comparison.


Microbial communities and chemosynthesis in yellowstone lake sublacustrine hydrothermal vent waters.

Yang T, Lyons S, Aguilar C, Cuhel R, Teske A - Front Microbiol (2011)

Photosynthesis-irradiance relationships at 15°C for surface waters overlying hydrothermal vent fields in Mary Bay (●), Stevenson Island (■), and West Thumb (▲).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3116135&req=5

Figure 2: Photosynthesis-irradiance relationships at 15°C for surface waters overlying hydrothermal vent fields in Mary Bay (●), Stevenson Island (■), and West Thumb (▲).
Mentions: Photosynthesis-irradiance curves for surface samples from the three domains of Mary Bay, Stevenson Island, and West Thumb demonstrated light-saturable photosynthetic CO2 fixation (Figure 2). Light stimulated photosynthetic rates to 200 μmol PAR photons m−2 s−1, about 10% of full sunlight, above which rates remained constant at 0.14 ± 0.01 μM C h−1. Because this discussion concerns volumetric carbon fixation, the data were not normalized for varying algal biomass as chlorophyll a and are presented in the same units as chemosynthesis for direct comparison.

Bottom Line: They harbor distinct chemosynthetic bacterial communities, depending on temperature (16-110°C) and electron donor supply (H(2)S <1 to >100 μM; NH(3) <0.5 to >10 μM).Vents at lower temperatures yielded mostly phylotypes related to the mesophilic gammaproteobacterial sulfur oxidizer Thiovirga.In contrast, cool vent water with low chemosynthetic activity yielded predominantly phylotypes related to freshwater Actinobacterial clusters with a cosmopolitan distribution.

View Article: PubMed Central - PubMed

Affiliation: Department of Marine Sciences, University of North Carolina at Chapel Hill Chapel Hill, NC, USA.

ABSTRACT
Five sublacustrine thermal spring locations from 1 to 109 m water depth in Yellowstone Lake were surveyed by 16S ribosomal RNA gene sequencing in relation to their chemical composition and dark CO(2) fixation rates. They harbor distinct chemosynthetic bacterial communities, depending on temperature (16-110°C) and electron donor supply (H(2)S <1 to >100 μM; NH(3) <0.5 to >10 μM). Members of the Aquificales, most closely affiliated with the genus Sulfurihydrogenibium, are the most frequently recovered bacterial 16S rRNA gene phylotypes in the hottest samples; the detection of these thermophilic sulfur-oxidizing autotrophs coincided with maximal dark CO(2) fixation rates reaching near 9 μM C h(-1) at temperatures of 50-60°C. Vents at lower temperatures yielded mostly phylotypes related to the mesophilic gammaproteobacterial sulfur oxidizer Thiovirga. In contrast, cool vent water with low chemosynthetic activity yielded predominantly phylotypes related to freshwater Actinobacterial clusters with a cosmopolitan distribution.

No MeSH data available.