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Effect of a dual drug-coated stent with abciximab and alpha-lipoic Acid in a porcine coronary restenosis model.

Lim KS, Hong YJ, Hachinohe D, Ahmed K, Jeong MH, Kim JH, Sim DS, Lee MG, Park KH, Kim JH, Ahn Y, Cho JG, Park JC, Song SJ, Jung KW, Cho DL, Kang JC - Korean Circ J (2011)

Bottom Line: There was no significant difference in the injury score between the two groups.In the neointima, the lymphohistiocyte count was significantly lower in dual-coat stent group compared with the control stent group (120±85 cells vs. 159±80 cells, p=0.048).Although the dual-coated stent with abciximab and ALA showed no significant difference in inhibition of neointimal hyperplasia when compared with the bare metal stent, it was associated with a reduced inflammatory reaction when compared with the control stent in a porcine coronary restenosis model.

View Article: PubMed Central - PubMed

Affiliation: The Heart Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare, Gwangju, Korea.

ABSTRACT

Background and objectives: The aim of this study was to examine the anti-proliferative and anti-inflammatory effects of a stent coated with abciximab and alpha-lipoic acid (ALA) in a porcine coronary overstretch restenosis model.

Materials and methods: A total of 10 pigs were randomized into two groups (10 pigs, 10 coronaries in each group) in which the coronary arteries were stented with a dual-coated stent and a bare metal stent (control) by randomization. Stents were deployed with oversizing (stent/artery ratio 1.3 : 1) in the porcine coronary arteries, and histopathology was assessed 28 days after stenting.

Results: There was no significant difference in the injury score between the two groups. In the neointima, the lymphohistiocyte count was significantly lower in dual-coat stent group compared with the control stent group (120±85 cells vs. 159±80 cells, p=0.048). There was no significant difference in the fibrin score between the two groups (0.16±0.34 in the dual-coated stent group vs. 0.25±0.48 in the control stent group, p=0.446). The neointima area was not significantly different between both groups (1.55±0.8 mm(2) in dual-coated stent group vs. 1.40±0.86 mm(2) in the control stent group, p=0.447).

Conclusion: Although the dual-coated stent with abciximab and ALA showed no significant difference in inhibition of neointimal hyperplasia when compared with the bare metal stent, it was associated with a reduced inflammatory reaction when compared with the control stent in a porcine coronary restenosis model.

No MeSH data available.


Related in: MedlinePlus

Dual-coated stented porcine coronary arteries (A: H&E staining: ×20, B: methyl methacrylate staining: ×20) and control stented porcine coronary arteries (C: H&E staining: ×20, C: methyl methacrylate staining: ×20).
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Figure 2: Dual-coated stented porcine coronary arteries (A: H&E staining: ×20, B: methyl methacrylate staining: ×20) and control stented porcine coronary arteries (C: H&E staining: ×20, C: methyl methacrylate staining: ×20).

Mentions: Histopathologic evaluation of each artery was performed by an experienced cardiovascular pathologist. The specimens were embedded in methylmethacrylate and sections were cut with a low-speed diamond wafer mounted to a Buehler Isomet saw (Buehler Ltd., Lake Bluff, IL, USA), leaving the stent wires intact in the cross sections to minimize potential artifacts caused by removal of the stent wires. Sections of 50 to 100 µm thickness were obtained at about 1 mm apart and stained with hematoxylin-eosin for histological analysis (Fig. 2). Measurements of the histopathologic sections were performed using a calibrated microscope, digital video imaging system, and microcomputer program (Visus 2000 Visual Image Analysis System, IMT Tech, CA, USA). Borders were manually traced for the lumen area, the area circumscribed by the internal elastic lamina, and the innermost border of the external elastic lamina (external elastic lamina area). Morphometric analysis of the neointimal area for a given vessel was calculated as the measured internal elastic lamina area minus the lumen area. Measurements were made on five cross-sections from the proximal and distal ends and the three midpoints of each stented segment. Histopathologic stenosis was calculated as 100×{1-(lesion lumen area/lesion internal elastic lamina area)}.


Effect of a dual drug-coated stent with abciximab and alpha-lipoic Acid in a porcine coronary restenosis model.

Lim KS, Hong YJ, Hachinohe D, Ahmed K, Jeong MH, Kim JH, Sim DS, Lee MG, Park KH, Kim JH, Ahn Y, Cho JG, Park JC, Song SJ, Jung KW, Cho DL, Kang JC - Korean Circ J (2011)

Dual-coated stented porcine coronary arteries (A: H&E staining: ×20, B: methyl methacrylate staining: ×20) and control stented porcine coronary arteries (C: H&E staining: ×20, C: methyl methacrylate staining: ×20).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3116101&req=5

Figure 2: Dual-coated stented porcine coronary arteries (A: H&E staining: ×20, B: methyl methacrylate staining: ×20) and control stented porcine coronary arteries (C: H&E staining: ×20, C: methyl methacrylate staining: ×20).
Mentions: Histopathologic evaluation of each artery was performed by an experienced cardiovascular pathologist. The specimens were embedded in methylmethacrylate and sections were cut with a low-speed diamond wafer mounted to a Buehler Isomet saw (Buehler Ltd., Lake Bluff, IL, USA), leaving the stent wires intact in the cross sections to minimize potential artifacts caused by removal of the stent wires. Sections of 50 to 100 µm thickness were obtained at about 1 mm apart and stained with hematoxylin-eosin for histological analysis (Fig. 2). Measurements of the histopathologic sections were performed using a calibrated microscope, digital video imaging system, and microcomputer program (Visus 2000 Visual Image Analysis System, IMT Tech, CA, USA). Borders were manually traced for the lumen area, the area circumscribed by the internal elastic lamina, and the innermost border of the external elastic lamina (external elastic lamina area). Morphometric analysis of the neointimal area for a given vessel was calculated as the measured internal elastic lamina area minus the lumen area. Measurements were made on five cross-sections from the proximal and distal ends and the three midpoints of each stented segment. Histopathologic stenosis was calculated as 100×{1-(lesion lumen area/lesion internal elastic lamina area)}.

Bottom Line: There was no significant difference in the injury score between the two groups.In the neointima, the lymphohistiocyte count was significantly lower in dual-coat stent group compared with the control stent group (120±85 cells vs. 159±80 cells, p=0.048).Although the dual-coated stent with abciximab and ALA showed no significant difference in inhibition of neointimal hyperplasia when compared with the bare metal stent, it was associated with a reduced inflammatory reaction when compared with the control stent in a porcine coronary restenosis model.

View Article: PubMed Central - PubMed

Affiliation: The Heart Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare, Gwangju, Korea.

ABSTRACT

Background and objectives: The aim of this study was to examine the anti-proliferative and anti-inflammatory effects of a stent coated with abciximab and alpha-lipoic acid (ALA) in a porcine coronary overstretch restenosis model.

Materials and methods: A total of 10 pigs were randomized into two groups (10 pigs, 10 coronaries in each group) in which the coronary arteries were stented with a dual-coated stent and a bare metal stent (control) by randomization. Stents were deployed with oversizing (stent/artery ratio 1.3 : 1) in the porcine coronary arteries, and histopathology was assessed 28 days after stenting.

Results: There was no significant difference in the injury score between the two groups. In the neointima, the lymphohistiocyte count was significantly lower in dual-coat stent group compared with the control stent group (120±85 cells vs. 159±80 cells, p=0.048). There was no significant difference in the fibrin score between the two groups (0.16±0.34 in the dual-coated stent group vs. 0.25±0.48 in the control stent group, p=0.446). The neointima area was not significantly different between both groups (1.55±0.8 mm(2) in dual-coated stent group vs. 1.40±0.86 mm(2) in the control stent group, p=0.447).

Conclusion: Although the dual-coated stent with abciximab and ALA showed no significant difference in inhibition of neointimal hyperplasia when compared with the bare metal stent, it was associated with a reduced inflammatory reaction when compared with the control stent in a porcine coronary restenosis model.

No MeSH data available.


Related in: MedlinePlus