Limits...
A metabolic biofuel cell: conversion of human leukocyte metabolic activity to electrical currents.

Justin GA, Zhang Y, Cui XT, Bradberry CW, Sun M, Sclabassi RJ - J Biol Eng (2011)

Bottom Line: SCE for the PMA (phorbol ester) activated primary cells, with a notable absence of a reduction peak was observed.HPLC confirmed the release of serotonin (5-HT) from the PMA activated primary cells.It is believed that serotonin, among other biochemical species released by the activated cells, contributes to the observed BFC currents.

View Article: PubMed Central - HTML - PubMed

Affiliation: Computational Diagnostics, Inc, Pittsburgh, PA, USA. bobs@cdi.com.

ABSTRACT
An investigation of the electrochemical activity of human white blood cells (WBC) for biofuel cell (BFC) applications is described. WBCs isolated from whole human blood were suspended in PBS and introduced into the anode compartment of a proton exchange membrane (PEM) fuel cell. The cathode compartment contained a 50 mM potassium ferricyanide solution. Average current densities between 0.9 and 1.6 μA cm-2 and open circuit potentials (Voc) between 83 and 102 mV were obtained, which were both higher than control values. Cyclic voltammetry was used to investigate the electrochemical activity of the activated WBCs in an attempt to elucidate the mechanism of electron transfer between the cells and electrode. Voltammograms were obtained for the WBCs, including peripheral blood mononuclear cells (PBMCs - a lymphocyte-monocyte mixture isolated on a Ficoll gradient), a B lymphoblastoid cell line (BLCL), and two leukemia cell lines, namely K562 and Jurkat. An oxidation peak at about 363 mV vs. SCE for the PMA (phorbol ester) activated primary cells, with a notable absence of a reduction peak was observed. Oxidation peaks were not observed for the BLCL, K562 or Jurkat cell lines. HPLC confirmed the release of serotonin (5-HT) from the PMA activated primary cells. It is believed that serotonin, among other biochemical species released by the activated cells, contributes to the observed BFC currents.

No MeSH data available.


Related in: MedlinePlus

Comparison of currents obtained from WBCs and PBMCs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3113927&req=5

Figure 4: Comparison of currents obtained from WBCs and PBMCs.

Mentions: The current output from PBMCs (isolated on a Ficoll-Paque ™ gradient) within the same experimental setup was also measured. The average current from the PBMC was slightly lower than that recorded for the general WBC population - which contains several other cell types, including neutrophils in addition to the PBMCs (Figure 4). A PEMFC containing PBS only (without activating agents) at the anode did not produce any observable currents or open circuit potentials (not shown). Introducing samples of the PBS supernatant, used in the initial washing of both the WBC and PBMC samples subsequent to isolation, into the anode compartment of the PEMFC produced very small to no currents (Figure 5). This further supports the theory that the cells, and not residual components from the cell isolation procedure, are primarily responsible for the observed currents. Frequent measurements of pH made during the course of all experiments also did not reveal any significant changes across all anode solution samples (pH maintained at 7.4 regardless of cell density, cell activation or due to the presence of the activating agents).


A metabolic biofuel cell: conversion of human leukocyte metabolic activity to electrical currents.

Justin GA, Zhang Y, Cui XT, Bradberry CW, Sun M, Sclabassi RJ - J Biol Eng (2011)

Comparison of currents obtained from WBCs and PBMCs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3113927&req=5

Figure 4: Comparison of currents obtained from WBCs and PBMCs.
Mentions: The current output from PBMCs (isolated on a Ficoll-Paque ™ gradient) within the same experimental setup was also measured. The average current from the PBMC was slightly lower than that recorded for the general WBC population - which contains several other cell types, including neutrophils in addition to the PBMCs (Figure 4). A PEMFC containing PBS only (without activating agents) at the anode did not produce any observable currents or open circuit potentials (not shown). Introducing samples of the PBS supernatant, used in the initial washing of both the WBC and PBMC samples subsequent to isolation, into the anode compartment of the PEMFC produced very small to no currents (Figure 5). This further supports the theory that the cells, and not residual components from the cell isolation procedure, are primarily responsible for the observed currents. Frequent measurements of pH made during the course of all experiments also did not reveal any significant changes across all anode solution samples (pH maintained at 7.4 regardless of cell density, cell activation or due to the presence of the activating agents).

Bottom Line: SCE for the PMA (phorbol ester) activated primary cells, with a notable absence of a reduction peak was observed.HPLC confirmed the release of serotonin (5-HT) from the PMA activated primary cells.It is believed that serotonin, among other biochemical species released by the activated cells, contributes to the observed BFC currents.

View Article: PubMed Central - HTML - PubMed

Affiliation: Computational Diagnostics, Inc, Pittsburgh, PA, USA. bobs@cdi.com.

ABSTRACT
An investigation of the electrochemical activity of human white blood cells (WBC) for biofuel cell (BFC) applications is described. WBCs isolated from whole human blood were suspended in PBS and introduced into the anode compartment of a proton exchange membrane (PEM) fuel cell. The cathode compartment contained a 50 mM potassium ferricyanide solution. Average current densities between 0.9 and 1.6 μA cm-2 and open circuit potentials (Voc) between 83 and 102 mV were obtained, which were both higher than control values. Cyclic voltammetry was used to investigate the electrochemical activity of the activated WBCs in an attempt to elucidate the mechanism of electron transfer between the cells and electrode. Voltammograms were obtained for the WBCs, including peripheral blood mononuclear cells (PBMCs - a lymphocyte-monocyte mixture isolated on a Ficoll gradient), a B lymphoblastoid cell line (BLCL), and two leukemia cell lines, namely K562 and Jurkat. An oxidation peak at about 363 mV vs. SCE for the PMA (phorbol ester) activated primary cells, with a notable absence of a reduction peak was observed. Oxidation peaks were not observed for the BLCL, K562 or Jurkat cell lines. HPLC confirmed the release of serotonin (5-HT) from the PMA activated primary cells. It is believed that serotonin, among other biochemical species released by the activated cells, contributes to the observed BFC currents.

No MeSH data available.


Related in: MedlinePlus