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Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons.

Fang X, Ma H, Lu D, Yu H, Lai W, Ruan S - Proteome Sci (2011)

Bottom Line: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes.Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Plant Molecular Biology and Proteomics, Institute of Biology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China. hzhsma@163.com.

ABSTRACT

Background: Strawberries (Fragaria ananassa) reproduce asexually through stolons, which have strong tendencies to form adventitious roots at their second node. Understanding how the development of the proximal (I-1) and distal (I-2) internodes of stolons differ should facilitate nursery cultivation of strawberries.

Results: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes. Proteins extracted from the internodes were separated by two-dimensional gel electrophoresis, and 164 I-1 protein spots and 200 I-2 protein spots were examined further. Using mass spectrometry and database searches, 38 I-1 and 52 I-2 proteins were identified and categorized (8 and 10 groups, respectively) according to their cellular compartmentalization and functionality. Many of the identified proteins are enzymes necessary for carbohydrate metabolism and photosynthesis. Furthermore, identification of proteins that interact revealed that many of the I-2 proteins form a dynamic network during development. Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.

Conclusions: Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

No MeSH data available.


Related in: MedlinePlus

Model for adventitious root and clonal plant formation in I-2 that incorporates four regulated pathways. Five identified I-2 proteins were integrated into the model, and the possible PPIs are shown (dashed lines) based on the PPI network in Figure 8. (a) Anaphase-promoting complex (APC/C) is a ubiquitin ligase that plays a key role in the cell cycle. (b) Eukaryotic translation initiation factor 5A (EIF-5A) may interact with PUB34 to regulate cell division. (c) ACS7, when interacting with ubiquitin ligase, plays a central role in ethylene biosynthesis. (d) Important regulatory effects on plant growth and development have been reported for trehalose (Tre) and trehalose 6-phosphate (T6P). (CDC20: cell-division cycle protein 20; G6P: glucose 6-phosphate; TPP: trehalose-6-phosphate phosphatase
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Figure 9: Model for adventitious root and clonal plant formation in I-2 that incorporates four regulated pathways. Five identified I-2 proteins were integrated into the model, and the possible PPIs are shown (dashed lines) based on the PPI network in Figure 8. (a) Anaphase-promoting complex (APC/C) is a ubiquitin ligase that plays a key role in the cell cycle. (b) Eukaryotic translation initiation factor 5A (EIF-5A) may interact with PUB34 to regulate cell division. (c) ACS7, when interacting with ubiquitin ligase, plays a central role in ethylene biosynthesis. (d) Important regulatory effects on plant growth and development have been reported for trehalose (Tre) and trehalose 6-phosphate (T6P). (CDC20: cell-division cycle protein 20; G6P: glucose 6-phosphate; TPP: trehalose-6-phosphate phosphatase

Mentions: Adventitious roots develop from the second node at the end of I-2 before clonal plant formation. We developed a model for adventitious root formation in I-2 based on published data [64-69] and our findings; the model includes four regulated pathways (Figure 9). Regulated protein degradation has repeatedly been identified as a key component of cell-cycle regulation. Securin inhibits a protease called separase, which cleaves cohesins allowing anaphase onset. Activated APCcdc20 targets securin for degradation, which initiates the metaphase-to-anaphase transition [68]. In addition, biochemical and molecular studies have shown that EIF-5A is crucial for plant growth and development as it regulates cell division and cell growth [69]. Continuous cell division, elongation, and differentiation can cause the formation of root primordia, so the APC complex-related and EIF-5A-related biological processes may be two important pathways that regulate the formation of adventitious roots. Moreover, ACS7 catalyzes the conversion of AdoMet into 1-aminocyclopropane-1-carboxylate, a direct precursor of ethylene, whereas ACS7 is ubiquitinated. Ubiquitination probably leads to its subsequent degradation, thus controlling ethylene production. Ethylene can regulate root initiation and emergence. Conversely, as an important catalytic enzyme, TPS4 plays a central role in the complex signaling network that links sugars and hormones with its interacting partner PUB34. Together, the four pathways work synergistically to induce formation of adventitious roots.


Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons.

Fang X, Ma H, Lu D, Yu H, Lai W, Ruan S - Proteome Sci (2011)

Model for adventitious root and clonal plant formation in I-2 that incorporates four regulated pathways. Five identified I-2 proteins were integrated into the model, and the possible PPIs are shown (dashed lines) based on the PPI network in Figure 8. (a) Anaphase-promoting complex (APC/C) is a ubiquitin ligase that plays a key role in the cell cycle. (b) Eukaryotic translation initiation factor 5A (EIF-5A) may interact with PUB34 to regulate cell division. (c) ACS7, when interacting with ubiquitin ligase, plays a central role in ethylene biosynthesis. (d) Important regulatory effects on plant growth and development have been reported for trehalose (Tre) and trehalose 6-phosphate (T6P). (CDC20: cell-division cycle protein 20; G6P: glucose 6-phosphate; TPP: trehalose-6-phosphate phosphatase
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3113925&req=5

Figure 9: Model for adventitious root and clonal plant formation in I-2 that incorporates four regulated pathways. Five identified I-2 proteins were integrated into the model, and the possible PPIs are shown (dashed lines) based on the PPI network in Figure 8. (a) Anaphase-promoting complex (APC/C) is a ubiquitin ligase that plays a key role in the cell cycle. (b) Eukaryotic translation initiation factor 5A (EIF-5A) may interact with PUB34 to regulate cell division. (c) ACS7, when interacting with ubiquitin ligase, plays a central role in ethylene biosynthesis. (d) Important regulatory effects on plant growth and development have been reported for trehalose (Tre) and trehalose 6-phosphate (T6P). (CDC20: cell-division cycle protein 20; G6P: glucose 6-phosphate; TPP: trehalose-6-phosphate phosphatase
Mentions: Adventitious roots develop from the second node at the end of I-2 before clonal plant formation. We developed a model for adventitious root formation in I-2 based on published data [64-69] and our findings; the model includes four regulated pathways (Figure 9). Regulated protein degradation has repeatedly been identified as a key component of cell-cycle regulation. Securin inhibits a protease called separase, which cleaves cohesins allowing anaphase onset. Activated APCcdc20 targets securin for degradation, which initiates the metaphase-to-anaphase transition [68]. In addition, biochemical and molecular studies have shown that EIF-5A is crucial for plant growth and development as it regulates cell division and cell growth [69]. Continuous cell division, elongation, and differentiation can cause the formation of root primordia, so the APC complex-related and EIF-5A-related biological processes may be two important pathways that regulate the formation of adventitious roots. Moreover, ACS7 catalyzes the conversion of AdoMet into 1-aminocyclopropane-1-carboxylate, a direct precursor of ethylene, whereas ACS7 is ubiquitinated. Ubiquitination probably leads to its subsequent degradation, thus controlling ethylene production. Ethylene can regulate root initiation and emergence. Conversely, as an important catalytic enzyme, TPS4 plays a central role in the complex signaling network that links sugars and hormones with its interacting partner PUB34. Together, the four pathways work synergistically to induce formation of adventitious roots.

Bottom Line: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes.Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Plant Molecular Biology and Proteomics, Institute of Biology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China. hzhsma@163.com.

ABSTRACT

Background: Strawberries (Fragaria ananassa) reproduce asexually through stolons, which have strong tendencies to form adventitious roots at their second node. Understanding how the development of the proximal (I-1) and distal (I-2) internodes of stolons differ should facilitate nursery cultivation of strawberries.

Results: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes. Proteins extracted from the internodes were separated by two-dimensional gel electrophoresis, and 164 I-1 protein spots and 200 I-2 protein spots were examined further. Using mass spectrometry and database searches, 38 I-1 and 52 I-2 proteins were identified and categorized (8 and 10 groups, respectively) according to their cellular compartmentalization and functionality. Many of the identified proteins are enzymes necessary for carbohydrate metabolism and photosynthesis. Furthermore, identification of proteins that interact revealed that many of the I-2 proteins form a dynamic network during development. Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.

Conclusions: Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

No MeSH data available.


Related in: MedlinePlus