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Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons.

Fang X, Ma H, Lu D, Yu H, Lai W, Ruan S - Proteome Sci (2011)

Bottom Line: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes.Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Plant Molecular Biology and Proteomics, Institute of Biology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China. hzhsma@163.com.

ABSTRACT

Background: Strawberries (Fragaria ananassa) reproduce asexually through stolons, which have strong tendencies to form adventitious roots at their second node. Understanding how the development of the proximal (I-1) and distal (I-2) internodes of stolons differ should facilitate nursery cultivation of strawberries.

Results: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes. Proteins extracted from the internodes were separated by two-dimensional gel electrophoresis, and 164 I-1 protein spots and 200 I-2 protein spots were examined further. Using mass spectrometry and database searches, 38 I-1 and 52 I-2 proteins were identified and categorized (8 and 10 groups, respectively) according to their cellular compartmentalization and functionality. Many of the identified proteins are enzymes necessary for carbohydrate metabolism and photosynthesis. Furthermore, identification of proteins that interact revealed that many of the I-2 proteins form a dynamic network during development. Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.

Conclusions: Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

No MeSH data available.


Related in: MedlinePlus

Possible protein-protein interaction network among I-2 proteins derived using the Cytoprophet module of Cytoscape. Cytoprophet draws a network of potential interactions with probability scores and GO distances as edge attributes. Proteins are marked with UniProt ID names.
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Figure 8: Possible protein-protein interaction network among I-2 proteins derived using the Cytoprophet module of Cytoscape. Cytoprophet draws a network of potential interactions with probability scores and GO distances as edge attributes. Proteins are marked with UniProt ID names.

Mentions: Although we could not use Cytoprophet to delineate a PPI network for I-1 because of limited information, a possible PPI network was found for I-2 (Figure 8). U-box domain-containing protein 34 (PUB34) (Q8S8S7_ARATH) is the central core protein of the signaling network, as it interacts with many other proteins, e.g., alpha, alpha-trehalose-phosphate synthase (TPS4) (Q9T079_ARATH), APC2 (Q8H1U5_ARATH), 1-aminocyclopropane-1-carboxylate synthase 7 (ACS7) (1A17_ ARATH), and EIF5A. APC2 is an E3 ubiquitin ligase that is a component of the SCF family ubiquitin ligases, which catalyze the attachment of ubiquitin to the lysine side chains of securin and mitotic cyclins [64-66]. EIF5A interacts with BRUSHY1 (BRU1_ARATH), which is required for the proper arrangement of cells in the root and shoot apical meristems. Ubiquitin-mediated protein degradation probably affects meristem structural formation by modulating the concentration of cell-cycle regulators and transcription factors [67]. Therefore, the ubiquitin system may be vital during the morphogenesis of clonal plants.


Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons.

Fang X, Ma H, Lu D, Yu H, Lai W, Ruan S - Proteome Sci (2011)

Possible protein-protein interaction network among I-2 proteins derived using the Cytoprophet module of Cytoscape. Cytoprophet draws a network of potential interactions with probability scores and GO distances as edge attributes. Proteins are marked with UniProt ID names.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3113925&req=5

Figure 8: Possible protein-protein interaction network among I-2 proteins derived using the Cytoprophet module of Cytoscape. Cytoprophet draws a network of potential interactions with probability scores and GO distances as edge attributes. Proteins are marked with UniProt ID names.
Mentions: Although we could not use Cytoprophet to delineate a PPI network for I-1 because of limited information, a possible PPI network was found for I-2 (Figure 8). U-box domain-containing protein 34 (PUB34) (Q8S8S7_ARATH) is the central core protein of the signaling network, as it interacts with many other proteins, e.g., alpha, alpha-trehalose-phosphate synthase (TPS4) (Q9T079_ARATH), APC2 (Q8H1U5_ARATH), 1-aminocyclopropane-1-carboxylate synthase 7 (ACS7) (1A17_ ARATH), and EIF5A. APC2 is an E3 ubiquitin ligase that is a component of the SCF family ubiquitin ligases, which catalyze the attachment of ubiquitin to the lysine side chains of securin and mitotic cyclins [64-66]. EIF5A interacts with BRUSHY1 (BRU1_ARATH), which is required for the proper arrangement of cells in the root and shoot apical meristems. Ubiquitin-mediated protein degradation probably affects meristem structural formation by modulating the concentration of cell-cycle regulators and transcription factors [67]. Therefore, the ubiquitin system may be vital during the morphogenesis of clonal plants.

Bottom Line: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes.Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Plant Molecular Biology and Proteomics, Institute of Biology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China. hzhsma@163.com.

ABSTRACT

Background: Strawberries (Fragaria ananassa) reproduce asexually through stolons, which have strong tendencies to form adventitious roots at their second node. Understanding how the development of the proximal (I-1) and distal (I-2) internodes of stolons differ should facilitate nursery cultivation of strawberries.

Results: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes. Proteins extracted from the internodes were separated by two-dimensional gel electrophoresis, and 164 I-1 protein spots and 200 I-2 protein spots were examined further. Using mass spectrometry and database searches, 38 I-1 and 52 I-2 proteins were identified and categorized (8 and 10 groups, respectively) according to their cellular compartmentalization and functionality. Many of the identified proteins are enzymes necessary for carbohydrate metabolism and photosynthesis. Furthermore, identification of proteins that interact revealed that many of the I-2 proteins form a dynamic network during development. Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.

Conclusions: Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

No MeSH data available.


Related in: MedlinePlus