Limits...
Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons.

Fang X, Ma H, Lu D, Yu H, Lai W, Ruan S - Proteome Sci (2011)

Bottom Line: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes.Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Plant Molecular Biology and Proteomics, Institute of Biology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China. hzhsma@163.com.

ABSTRACT

Background: Strawberries (Fragaria ananassa) reproduce asexually through stolons, which have strong tendencies to form adventitious roots at their second node. Understanding how the development of the proximal (I-1) and distal (I-2) internodes of stolons differ should facilitate nursery cultivation of strawberries.

Results: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes. Proteins extracted from the internodes were separated by two-dimensional gel electrophoresis, and 164 I-1 protein spots and 200 I-2 protein spots were examined further. Using mass spectrometry and database searches, 38 I-1 and 52 I-2 proteins were identified and categorized (8 and 10 groups, respectively) according to their cellular compartmentalization and functionality. Many of the identified proteins are enzymes necessary for carbohydrate metabolism and photosynthesis. Furthermore, identification of proteins that interact revealed that many of the I-2 proteins form a dynamic network during development. Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.

Conclusions: Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

No MeSH data available.


Related in: MedlinePlus

Venn diagram for the identified I-1 and I-2 proteins. The numbers and percentages of unique proteins (excluding isoforms) found for either or both internodes are given. Spot numbers for I-1 proteins are given first followed by spot numbers for the corresponding I-2 proteins in parentheses.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3113925&req=5

Figure 3: Venn diagram for the identified I-1 and I-2 proteins. The numbers and percentages of unique proteins (excluding isoforms) found for either or both internodes are given. Spot numbers for I-1 proteins are given first followed by spot numbers for the corresponding I-2 proteins in parentheses.

Mentions: For protein identification, the peptide mass fingerprinting data were used in conjunction with a search of the complete Swissprot Viridiplantae taxonomic database as only 1017 F. ananassa protein sequences were available therein. Respectively, 164 and 200 spots from the I-1 and I-2 gels were selected for MS, and 38 and 52 proteins were identified, i.e., ~25% of the total in each case (Tables 1 and 2). No unambiguous matches were made for the other 274 proteins, probably because the proteins were not included in the database or because a protein spot contained more than one protein. For the 90 identified proteins, 5 (6.5%) were common to both proteomes, which indicated that the two internodes had some proteins (probably housekeeping proteins) in common. Certain proteins (35% of the gene products) were specific to I-1, whereas 58.5% were specific to I-2 (Figure 3). More than 50% of the proteins could be correlated with annotated proteins from at least one dicotyledon species (Figure 4). Of the F. ananassa proteins identified in our study, 13 I-1 and 25 I-2 proteins were matched to Arabidopsis thaliana proteins, and others were matched to Oryza sativa proteins (two from I-1, six from I-2) and Zea mays proteins (three from I-1, two from I-2). Only five I-l proteins and three I-2 proteins could be matched to those found in the F. ananassa database, possibly because the number of annotated proteins contained in the database is relatively small compared with that for the A. thaliana database.


Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons.

Fang X, Ma H, Lu D, Yu H, Lai W, Ruan S - Proteome Sci (2011)

Venn diagram for the identified I-1 and I-2 proteins. The numbers and percentages of unique proteins (excluding isoforms) found for either or both internodes are given. Spot numbers for I-1 proteins are given first followed by spot numbers for the corresponding I-2 proteins in parentheses.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3113925&req=5

Figure 3: Venn diagram for the identified I-1 and I-2 proteins. The numbers and percentages of unique proteins (excluding isoforms) found for either or both internodes are given. Spot numbers for I-1 proteins are given first followed by spot numbers for the corresponding I-2 proteins in parentheses.
Mentions: For protein identification, the peptide mass fingerprinting data were used in conjunction with a search of the complete Swissprot Viridiplantae taxonomic database as only 1017 F. ananassa protein sequences were available therein. Respectively, 164 and 200 spots from the I-1 and I-2 gels were selected for MS, and 38 and 52 proteins were identified, i.e., ~25% of the total in each case (Tables 1 and 2). No unambiguous matches were made for the other 274 proteins, probably because the proteins were not included in the database or because a protein spot contained more than one protein. For the 90 identified proteins, 5 (6.5%) were common to both proteomes, which indicated that the two internodes had some proteins (probably housekeeping proteins) in common. Certain proteins (35% of the gene products) were specific to I-1, whereas 58.5% were specific to I-2 (Figure 3). More than 50% of the proteins could be correlated with annotated proteins from at least one dicotyledon species (Figure 4). Of the F. ananassa proteins identified in our study, 13 I-1 and 25 I-2 proteins were matched to Arabidopsis thaliana proteins, and others were matched to Oryza sativa proteins (two from I-1, six from I-2) and Zea mays proteins (three from I-1, two from I-2). Only five I-l proteins and three I-2 proteins could be matched to those found in the F. ananassa database, possibly because the number of annotated proteins contained in the database is relatively small compared with that for the A. thaliana database.

Bottom Line: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes.Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Plant Molecular Biology and Proteomics, Institute of Biology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China. hzhsma@163.com.

ABSTRACT

Background: Strawberries (Fragaria ananassa) reproduce asexually through stolons, which have strong tendencies to form adventitious roots at their second node. Understanding how the development of the proximal (I-1) and distal (I-2) internodes of stolons differ should facilitate nursery cultivation of strawberries.

Results: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes. Proteins extracted from the internodes were separated by two-dimensional gel electrophoresis, and 164 I-1 protein spots and 200 I-2 protein spots were examined further. Using mass spectrometry and database searches, 38 I-1 and 52 I-2 proteins were identified and categorized (8 and 10 groups, respectively) according to their cellular compartmentalization and functionality. Many of the identified proteins are enzymes necessary for carbohydrate metabolism and photosynthesis. Furthermore, identification of proteins that interact revealed that many of the I-2 proteins form a dynamic network during development. Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.

Conclusions: Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

No MeSH data available.


Related in: MedlinePlus