Limits...
Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons.

Fang X, Ma H, Lu D, Yu H, Lai W, Ruan S - Proteome Sci (2011)

Bottom Line: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes.Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Plant Molecular Biology and Proteomics, Institute of Biology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China. hzhsma@163.com.

ABSTRACT

Background: Strawberries (Fragaria ananassa) reproduce asexually through stolons, which have strong tendencies to form adventitious roots at their second node. Understanding how the development of the proximal (I-1) and distal (I-2) internodes of stolons differ should facilitate nursery cultivation of strawberries.

Results: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes. Proteins extracted from the internodes were separated by two-dimensional gel electrophoresis, and 164 I-1 protein spots and 200 I-2 protein spots were examined further. Using mass spectrometry and database searches, 38 I-1 and 52 I-2 proteins were identified and categorized (8 and 10 groups, respectively) according to their cellular compartmentalization and functionality. Many of the identified proteins are enzymes necessary for carbohydrate metabolism and photosynthesis. Furthermore, identification of proteins that interact revealed that many of the I-2 proteins form a dynamic network during development. Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.

Conclusions: Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

No MeSH data available.


Related in: MedlinePlus

The strawberry stolon. As shown, a stolon shoots away from the base of a strawberry plant. A clone is formed at a variable distance away from the parent at the second node concomitant with adventitious root formation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3113925&req=5

Figure 1: The strawberry stolon. As shown, a stolon shoots away from the base of a strawberry plant. A clone is formed at a variable distance away from the parent at the second node concomitant with adventitious root formation.

Mentions: Several stoloniferous species produce long, sympodial stolons with rooted rosettes (ramets) at their nodes [1,2]. For the garden strawberry (Fragaria ananassa), the mother plant forms plantlets on stolons during spring growth (Figure 1). The first stolon originates from an auxiliary leaf bud produced in the central crown and commonly contains only two nodes. The regions along the stolon and between the plant and the first node and the first and second nodes are the I-1 (proximal) and I-2 (distal) internodes, respectively. Although stolon growth requires internode elongation, the fates of the two nodes are dissimilar. First, I-1 elongates and terminates at the first node, which is nonproductive, then I-2 elongates and terminates at the second node, which forms the main crown of the clonal plant.


Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons.

Fang X, Ma H, Lu D, Yu H, Lai W, Ruan S - Proteome Sci (2011)

The strawberry stolon. As shown, a stolon shoots away from the base of a strawberry plant. A clone is formed at a variable distance away from the parent at the second node concomitant with adventitious root formation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3113925&req=5

Figure 1: The strawberry stolon. As shown, a stolon shoots away from the base of a strawberry plant. A clone is formed at a variable distance away from the parent at the second node concomitant with adventitious root formation.
Mentions: Several stoloniferous species produce long, sympodial stolons with rooted rosettes (ramets) at their nodes [1,2]. For the garden strawberry (Fragaria ananassa), the mother plant forms plantlets on stolons during spring growth (Figure 1). The first stolon originates from an auxiliary leaf bud produced in the central crown and commonly contains only two nodes. The regions along the stolon and between the plant and the first node and the first and second nodes are the I-1 (proximal) and I-2 (distal) internodes, respectively. Although stolon growth requires internode elongation, the fates of the two nodes are dissimilar. First, I-1 elongates and terminates at the first node, which is nonproductive, then I-2 elongates and terminates at the second node, which forms the main crown of the clonal plant.

Bottom Line: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes.Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Plant Molecular Biology and Proteomics, Institute of Biology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China. hzhsma@163.com.

ABSTRACT

Background: Strawberries (Fragaria ananassa) reproduce asexually through stolons, which have strong tendencies to form adventitious roots at their second node. Understanding how the development of the proximal (I-1) and distal (I-2) internodes of stolons differ should facilitate nursery cultivation of strawberries.

Results: Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes. Proteins extracted from the internodes were separated by two-dimensional gel electrophoresis, and 164 I-1 protein spots and 200 I-2 protein spots were examined further. Using mass spectrometry and database searches, 38 I-1 and 52 I-2 proteins were identified and categorized (8 and 10 groups, respectively) according to their cellular compartmentalization and functionality. Many of the identified proteins are enzymes necessary for carbohydrate metabolism and photosynthesis. Furthermore, identification of proteins that interact revealed that many of the I-2 proteins form a dynamic network during development. Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node.

Conclusions: Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.

No MeSH data available.


Related in: MedlinePlus